GdF3 as a promising phosphopeptide affinity probe and dephospho-labelling medium: experiments and theoretical explanation.

Bone-like GdF3 was synthesized and applied for phosphopeptide enrichment for the first time. As a new kind of efficient phosphopeptide affinity probe, GdF3 exhibits high efficiency in the mediation of the dephosphorylation reaction. In addition, DFT calculations were introduced to theoretically explain the unique property of GdF3 compared to GdPO4, which is promising and can be potentially significant in protein phosphorylation research.

Guanidyl-functionalized graphene as a bifunctional adsorbent for selective enrichment of phosphopeptides.

Guanidyl-functionalized graphene (GFG) was designed and synthesized. By simple modulation of the loading buffer this novel bifunctional adsorbent provides two enriching functions, one for global phosphopeptides, and the other for multiphosphopeptides with consecutive phosphorylated residues. GFG provides a promising perspective for consecutive phosphorylated peptide enrichment in phosphor-proteomic research and related biological process studies.

SnO(2)-ZnSn(OH)(6): a novel binary affinity probe for global phosphopeptide detection.

ZnSn(OH)(6) and binary-component SnO(2)-ZnSn(OH)(6) were introduced as affinity probes for phosphopeptide enrichment for the first time. Two strategies, either ZnSn(OH)(6) and SnO(2) serial enrichment or binary-component SnO(2)-ZnSn(OH)(6) enrichment in a single run, were proposed to enhance multi-phosphopeptide enrichment and to significantly improve global phosphopeptide detection.