A three-lncRNA expression signature predicts survival in head and neck squamous cell carcinoma (HNSCC).

Increasing evidence has shown that long non-coding RNAs (lncRNAs) have important biological functions and can be used as a prognostic biomarker in human cancers. However, investigation of the prognostic value of lncRNAs in head and neck squamous cell carcinoma (HNSCC) is in infancy. In the present study, we analyzed the lncRNA expression data in a large number of HNSCC patients (=425) derived from The Cancer Genome Atlas (TCGA) to identify an lncRNA expression signature for improving the prognosis of HNSCC.

Comprehensive analysis of the whole coding and non-coding RNA transcriptome expression profiles and construction of the circRNA-lncRNA co-regulated ceRNA network in laryngeal squamous cell carcinoma.

Recently, accumulating evidence has demonstrated that non-coding RNAs (ncRNAs) play a vital role in oncogenicity. Nevertheless, the regulatory mechanisms and functions remain poorly understood, especially for lncRNAs and circRNAs. In this study, we simultaneously detected, for the first time, the expression profiles of the whole transcriptome, including miRNA, circRNA and lncRNA + mRNA, in five pairs of laryngeal squamous cell carcinoma (LSCC) and matched non-carcinoma tissues by microarrays.

MicroRNA-101 is a potential prognostic indicator of laryngeal squamous cell carcinoma and modulates CDK8.

Background: Various microRNAs (miRNAs) negatively modulate genes that are involved in cellular proliferation, differentiation, invasion, and apoptosis. In many types of cancer, the expression profiles of these miRNAs are altered. Recently, miR-101 was identified as a tumour suppressor and was found to be expressed at low levels in various types of tumours, including prostate, breast, endometrium, and bladder cancers.

MiR-19a is correlated with prognosis and apoptosis of laryngeal squamous cell carcinoma by regulating TIMP-2 expression.

MiRNAs are small, noncoding RNA molecules that act as posttranscriptional regulators of gene expression and function as important regulators in cancer-related processes. The miR-19a is overexpressed in various cancers and has been causally related to cellular proliferation and growth. To determine whether miR-19a plays a role in laryngeal squamous cell carcinoma (LSCC), we used quantitative real time PCR to detect miR-19a expression in LSCC tissues.

Down-regulation of miR-129-5p inhibits growth and induces apoptosis in laryngeal squamous cell carcinoma by targeting APC.

miRNAs regulate gene expression and are key mediators of tumourigenesis. miR-129 has diverse effects in tumours, but its role in laryngeal squamous cell carcinoma (LSCC) remains unknown. This article focuses on the role of miR-129-5p in LSCC.

[Role of siRNA mediated matrix metalloproteinase-2 gene silencing in the inhibition of invasion and growth of laryngeal cancer cells].

Objective: To explore the inhibitory effect of matrix metalloproteinase-2 (MMP-2) gene silencing in vitro and in vivo on the invasion and growth of laryngeal cancer cells.

Methods: siRNA recombinant lentivirus targeting MMP-2 gene was transfected into Hep-2 cells, and MMP-2 protein expression was analyzed consequently by using western-blot. Invasive properties of transfectants were evaluated by Boyden assay.

[The experimental investigation of the invasion and growth of laryngeal cancer by matrix metalloproteinase-2 and matrix metalloproteinase-9 gene silence together].

Objective: To investigate the inhibitive role of the invasion and growth of laryngeal cancer by lentivirus mediated Matrix Metalloproteinase-2 (MMP-2) and Matrix Metalloproteinase-2 (MMP-9) gene silence.

Methods: RNA interference (RNAi) technic was used. Recombinant lentivirus of small interference RNA targeting MMP-2 and MMP-9 gene were transduced into Hep-2 cells, and Reverse transcriptase polymerase chain reaction (RT-PCR) was used to test MMP-2 and MMP-9 expression in Hep-2 cells.