Publications by authors named "Lin-Kai Huang"

Chinese chives is a popular herb vegetable and medicine in Asian countries. Southwest China is one of the centers of origin, and the mountainous areas in this region are rich in wild germplasm. In this study, we collected four samples of germplasm from different altitudes: a land race of cultivated Chinese chives (), wide-leaf chives and extra-wide-leaf chives (), and ovoid-leaf chives ().

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Gattinoni's equation, [Formula: see text], now commonly used to calculate the mechanical power (MP) of ventilation. However, it calculates only inspiratory MP. In addition, the inclusion of PEEP in Gattinoni's equation raises debate because PEEP does not produce net displacement or contribute to MP.

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This study investigated the usefulness of ventilator parameters in the prediction of development and outcome of acute respiratory distress syndrome (ARDS) in postoperative patients with esophageal or lung cancer on admission to the surgical intensive care unit (SICU). A total of 32 post-operative patients with lung or esophageal cancer from SICU in a tertiary medical center were retrospectively analyzed. The study patients were divided into an ARDS group ( = 21) and a non-ARDS group ( = 11).

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Cryptococcal meningitis (CM) is the most fatal adult meningitis in patients with human immunodeficiency virus (HIV). There is no conclusive evidence for the superiority of 1-week amphotericin B deoxycholate (AmphB) + flucytosine (5-FC) regimen over other antifungals in the management of HIV patients with CM (HIV-CM patients). We aimed to evaluate the differences in efficacy and tolerability of different antifungal agents in HIV-CM patients by conducting a current network meta-analysis NMA.

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Diabetes mellitus (DM) and tuberculosis (TB) are major public health and economic burdens. DM increases Mycobacterium tuberculosis (M.tb) infection rates and treatment durations.

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  • The study investigates the genetic diversity of Nevski Hand.-Mazz, a crucial perennial grass species, to enhance the conservation and utilization of genetic resources in western China.
  • Researchers utilized AFLP markers, resulting in 554 polymorphic fragments; they found that genetic relationships among 27 accessions were not strictly aligned with geographic locations, indicating complex population structures.
  • The findings highlighted significant genetic differentiation between geographic groups, with ecological factors, specifically average annual precipitation, impacting genetic diversity primarily in certain regions.
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The interaction of chitosan and polyamines (PAs) could be involved mitigating drought stress in white clover (Trifolium repens L.). This research aimed to determine the effect of chitosan and PAs, and co-application of chitosan and PAs on improving drought tolerance associated with growth, phytohormones, polyamines and antioxidant metabolism.

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Introduction: The optimal dose of inhaled metered-dose bronchodilators for intubated patients with chronic obstructive pulmonary disease (COPD) is unknown. In this study, we proposed a bronchodilator dosing schedule based on an individual's airway resistance (R) and tested its efficacy in reducing R.

Methods: A total of 51 newly admitted patients with invasively ventilated COPD were randomly assigned to receive personalized or fixed bronchodilator dosing.

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In order to investigate the physiological effects of exogenous γ-aminobutyric acid (GABA) on drought tolerance in white clover (Trifolium repens), GABA shunt, polyamines (PAs), and proline (Pro) metabolism were examined after plants pretreated with or without GABA (8 mM) and then exposed to water or 15% PEG-induced drought stress in growth chamber. In this study, exogenous application of GABA effectively alleviated drought-induced damage in leaves, as reflected by significantly higher relative water content, lower electrolyte leakage, lipid peroxidation, and leaf wilt. Exogenous GABA further promoted drought-induced increases in GABA transaminase and alpha ketone glutarate dehydrogenase activities, but inhibited glutamate decarboxylase activity under both control and drought conditions, resulting in an increase in endogenous glutamate (Glu) and GABA content.

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Drought is a major environmental stress that limits growth and development of cool-season annual grasses. Drought transcriptional profiles of resistant and susceptible lines were studied to understand the molecular mechanisms of drought tolerance in annual ryegrass (Lolium multiflorum L.).

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  • Hemarthria R. Br. is a key genus of perennial forage grasses, essential for animal husbandry in subtropical and tropical regions, but lacks comprehensive genomic data.
  • Using Illumina sequencing, researchers analyzed two important Hemarthria species, H. compressa "Yaan" and H. altissima "1110," generating over 24 million high-quality reads and identifying many unigenes and genetic markers.
  • The study identified significant SNPs and SSRs, developed PCR primers, and confirmed their effectiveness, thereby enhancing genomic knowledge of Hemarthria and enabling future genetic research.
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Simple sequence repeat (SSR) markers are widely applied in studies of plant molecular genetics due to their abundance in the genome, codominant nature, and high repeatability. However, microsatellites are not always available for the species to be studied and their isolation could be time- and cost-consuming. To investigate transferability in cross-species applications, 102 primer pairs previously developed in ryegrass and tall fescue were amplified across three allogamous ryegrass species including Lolium rigidum, Lolium perenne and Lolium multiflorum.

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  • The research explored how applying spermine (Spm) helps two types of white clover cope with drought stress, looking at carbohydrate metabolism and dehydrin gene expression.
  • Spm reduced the negative impacts of drought by increasing water-soluble carbohydrates and sugars in both clover types, with a notable rise in glucose in the drought-resistant 'Haifa' variety only.
  • The study found that Spm affects key enzyme activities related to sugar metabolism and promotes the accumulation of dehydrins, with differences in gene expression timing between the drought-susceptible 'Ladino' and the drought-resistant 'Haifa' cultivars.
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  • Start codon targeted polymorphism (SCoT) analysis identified 37 whipgrass clones, revealing significant genetic diversity and population structure among them.
  • The study generated 368 genetic fragments using 25 primer sets, with 77.21% of these fragments being polymorphic, indicating considerable variation among the clones.
  • Results showed no correlation between genetic relationships and geographic origins, highlighting that most genetic diversity resides within geographic groups rather than between them, supporting SCoT as a useful method for genetic characterization.
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  • This study investigated how exogenous spermidine (Spd) at 30 μM affects white clover seed germination under water stress induced by polyethylene glycol 6000.
  • Seeds treated with Spd showed improved germination rates, vigor, and overall growth metrics compared to control seeds treated with distilled water.
  • Enhanced starch metabolism and increased antioxidant enzyme activities in Spd-treated seeds contributed to better water stress tolerance and reduced damage from reactive oxygen species during germination.
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The present study was designed to examine the effects of heat acclimation on enzymatic activity, transcription levels, the photosynthesis processes associated with thermostability in orchardgrass (Dactylis glomerata L.).The stomatal conductance (Gs), net photosynthetic rate (Pn), and transpiration rates (Tr) of both heat-acclimated (HA) and non-acclimated (NA) plants were drastically reduced during heat treatment [using a 5-day heat stress treatment (38/30 °C ‒ day/night) followed by a 3-day recovery under control conditions (25/20 °C ‒ day/night), in order to consolidate the second cycle was permitted].

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  • * Key genetic metrics include an average of 70.59% polymorphic loci and a gene diversity (H) of 0.2589, indicating significant genetic variation among and within populations.
  • * Five distinct groups were identified through population structure and phylogenetic analysis, suggesting that human activity has influenced the genetic structure, which is important for breeding and conservation of M. sinensis for biofuel use.
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  • A study evaluated the impact of naphthalene acetic acid (NAA) on the rooting ability of Hemarthria compressa cuttings under controlled conditions.
  • Five NAA concentrations were tested from 0 to 400 mg/l, alongside three soaking durations (10, 20, and 30 minutes), with results indicating that 200 mg/l NAA soaked for 20 minutes yielded the best rooting outcomes.
  • Physiological changes measured included IAA-oxidase (IAAO), peroxidase (POD), and polyphenol oxidase (PPO) activity, showing that lower IAAO correlated with better rooting ability, suggesting potential markers for assessing root development in plant cuttings.
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Taking the drought-sensitive Dactylis glomerata line "01998" and drought-tolerant cultivar "Baoxing" as test materials, a pot experiment was conducted to study the effects of drought stress on the seedlings root growth, physiological characteristics, and leaf photosynthesis, aimed to approach the differences in the drought-tolerance mechanism of different D. glomerata lines (cultivars). Under drought stress, the root vitality and root number of "01998" and "Baoxing" presented a trend of increased first and decreased then.

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Background: The usual management of ventilator-associated pneumothorax (VPX) is tube thoracostomy. However, this recommendation is based on tradition rather than on solid evidence. Although it has been applied successfully to other types of pneumothoraces, observation has not been used in the management of VPX.

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Background: Even though there are continually upgraded recommendations for managing sepsis, such as "Surviving Sepsis Campaign: international guidelines for management of severe sepsis and septic shock", mortality is still high. Si-ni-tang, a remedy documented in Shanghan Lun, a medical collection from ancient China, is used for treating patients with sepsis and septic shock. Using a well-designed clinical trial, we are eager to survey the effectiveness of the concurrent use of this remedy in restoring these patients' hemodynamic status, or "Yang Qi".

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  • Soluble Fas ligand (sFasL) levels were found to be significantly higher in patients with tuberculous pleurisy compared to those without it, with a median concentration of 57.3 pg/mL versus 27.4 pg/mL.
  • When using a cutoff of 39.85 pg/mL, sFasL demonstrated a sensitivity of 95.7% and specificity of 80.4%, indicating its strong diagnostic potential.
  • The study concludes that measuring sFasL in pleural effusions can serve as an effective diagnostic marker for tuberculosis pleurisy.
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Toona sinensis extracts have been shown to exhibit anti-cancer effects in human ovarian cancer cell lines, human promyelocytic leukemia cells and human lung adenocarcinoma. Its safety has also been confirmed in animal studies. However, its anti-cancer properties in human lung large cell carcinoma have not been studied.

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Genetic variation and relationship of 53 accessions of D. glomerata collected from 5 continents were analyzed using simple sequence repeat (SSR) molecular markers with 15 SSR primer pairs. The following results were obtained.

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