An Investigation on the Molecular Characteristics and Intracellular Growth Ability among Environmental and Clinical Isolates of Legionella pneumophila in Sichuan Province, China.

Objective: To investigate the molecular characteristics and intracellular growth ability of Legionella pneumophila (L. pneumophila) strains from 1989 to 2016 in Sichuan Province, China.

Methods: Seventy-nine isolates of L.

[Preparation of recombinant Mycobacterium tuberculosis ESAT6-PPE68 fusion protein].

Objective: To construct esat6-ppe68 fusion gene and its prokaryotic expression vector for expression in E. coli.

Methods: With GeneSOEing method, a fusion gene was constructed by splicing esat6 gene and ppe68 gene and cloned into pGEX-4T-1 plasmid to construct the recombinant prokaryotic expression plasmid pGesat6-ppe68.