Targeted sequencing reveals the mutational landscape responsible for sorafenib therapy in advanced hepatocellular carcinoma.

: The existence of primary and acquired drug resistance is the main obstacle for the effect of multi-kinase inhibitor sorafenib and regorafenib in advanced hepatocellular carcinoma (HCC). However, plenty of patients did not significantly benefit from sorafenib treatment and little is known about the mechanism of drug resistance. : Laser capture microdissection was used to acquire matched normal liver and tumor tissues on formalin-fixed paraffin-embedded specimens collected before sorafenib therapy from the first surgery of 119 HCC patients.

A case of group infections with Paraginimus species in Henan, Central China.

In July of 2012, mass infections with Paragonimus species were detected in the Henan province sickening 11 of 51 people. In May 2011, these individuals had participated in an excursion during which freshwater crabs were caught and served after being toasted. Before the group infections with Paraginimus species was confirmed, 5 of the 11 patients had been misdiagnosed as tuberculosis (TB) and treated with an anti-TB drug regimen for six months.

Circular RNAs negatively regulate cancer stem cells by physically binding FMRP against CCAR1 complex in hepatocellular carcinoma.

Circular RNA (circRNA) possesses great pre-clinical diagnostic and therapeutic potentials in multiple cancers. It has been reported playing roles in multiple malignant behaviors including proliferation, migration, metastasis and chemoresistance. However, the underlying correlation between circRNAs and cancer stem cells (CSCs) has not been reported yet.

[Investigation on human intestinal parasitic diseases in ecological regions of Qinba Mountains in Henan Province in 2015].

Objective: To understand and analyze the epidemic status of human intestinal parasitic diseases in the ecological region of the Qinba Mountains in Henan Province, so as to provide the evidence for the control of the diseases in this region.

Methods: According to the methods of , 26 survey spots were selected in 8 counties (cities) in ecological regions of Qinba Mountains in Henan Province in 2015. After collecting the human fecal samples, the eggs of intestinal helminthes were detected with the modified Kato-Katz thick smear method, the species of of the hookworm egg-positive samples were identified with the tube fecal culture method, the eggs of were detected by using the adhesive tape method in children aged 3 to 6 years, and the cyst and trophozoite of intestinal protozoa were examined by iodine staining.

[Survey and analysis of epidemic status of principal human parasitosis in ecological region of Huaiyang hills of Henan Province in 2015].

Objective: To understand the epidemic status of principal human parasitosis in the ecological region of Huaiyang hills of Henan Province.

Methods: According to the scheme of The 3rd National Survey of Principal Human Parasites made by National Institute of Parasitic Diseases, the survey was performed based on the ecological regions. The stratified cluster sampling was made combined with the economic and geographical conditions.

A neglected risk for sparganosis: eating live tadpoles in central China.

A 29-year-old farmer from central China was sent into the Emergency Department of the Affiliated Hospital of Zhengzhou University. He had a 15-day history of persistent high fever, abdominal distention and pain. The patient was clinically diagnosed as appendicitis and peritonitis, and treated with antibiotics in a local hospital, did not improve.

Choline Kinase α Mediates Interactions Between the Epidermal Growth Factor Receptor and Mechanistic Target of Rapamycin Complex 2 in Hepatocellular Carcinoma Cells to Promote Drug Resistance and Xenograft Tumor Progression.

Background & Aims: Choline kinase α (CHKA) catalyzes conversion of choline to phosphocholine and can contribute to carcinogenesis. Little is known about the role of CHKA in the pathogenesis of hepatocellular carcinoma (HCC).

Methods: We performed whole-exome and transcriptome sequence analyses of 9 paired HCC and non-tumor-adjacent tissues.

[The Levels of IL-4, IL-9, and IgE in Patients Infected with Intestinal Helminths and their Clinical Values].

Objective: To investigate the serum levels of interleukin-4 (IL-4), interleukin-9 (IL-9), and immunoglobulin E (IgE) in the patients infected with intestinal helminths, and study their relationship to the clinical symptoms or species of the helminths.

Methods: This study was carried out in the Department of Paediatrics, Henan Provincial People's Hospital from January 2010 to July 2014. The blood samples were collected from 55 infected patients.

[Investigation on prevalence of Spirometra mansoni sparganum infection in frogs from Henan Province].

Objective: To understand the prevalence of Spirometra mansoni sparganum infection in frogs from Henan Province and analyze the risk of people suffering from sparganum infection.

Methods: The spargana were examined in the muscle of frogs collected from 12 sites of 5 regions in Henan Province, and the amount of spargana and their location were recorded.

Results: The natural infection rate of Spirometra mansoni spargana in frogs was 26.

Serodiagnosis of experimental sparganum infections of mice and human sparganosis by ELISA using ES antigens of Spirometra mansoni spargana.

We conducted a study of serodiagnosis of experimental sparganum infections of mice and human sparganosis by enzyme-linked immunosorbent assay (ELISA) using excretory-secretory (ES) antigens of Spirometra mansoni spargana and compared the sensitivity and specificity of crude and ES antigens for detecting the specific anti-sparganum IgG antibodies. By crude antigen ELISA and ES antigen ELISA, anti-sparganum IgG was detected in all of 30 serum samples of the infected mice; no cross-reactions were observed in serum samples of the mice infected with Trichinella spiralis, Schistosoma japanicum, Toxoplasma gondii, and normal mice. Anti-sparganum IgG was detected by ES antigen ELISA in sera of mice infected with one, two, four, six, and eight spargana at 3 weeks post-infection (wpi), with a detection rate of 100%, and lasted to 18 wpi when the experiment was ended.

[Epidemiological investigation on sparganosis mansoni and animal experiments].

The investigation was made in Luohe City of Henan Province from April to November, 2008. Cyclops were collected and plerocercoids of Spirometra mansoni were examined by microscope. Skin, muscle and internal organs of frogs and tadpoles were checked to detect spargana by naked eye and/or anatomical microscope.

[Study on the methods for detection of Toxoplasma antigen].

A sandwich ELISA was established with monoclonal antibody as detecting antibody and rabbit anti-Toxoplasma polyclonal antibody as capturing antibody. ABC (avidin-biotin-peroxidase complex)-ELISA and immuno-PCR were also used to detect different concentrations of Toxoplasma antigen. The lowest concentration of antigen to be detected by sandwich ELISA, ABC-ELISA and immuno-PCR was 0.