Bioluminescent Pseudomonas aeruginosa and Escherichia coli for whole-cell screening of antibacterial and adjuvant compounds.

Continued efforts to discover new antibacterial molecules are critical to achieve a robust pre-clinical pipeline for new antibiotics. Screening of compound or natural product extract libraries remains a widespread approach and can benefit from the development of whole cell assays that are robust, simple and versatile, and allow for high throughput testing of antibacterial activity. In this study, we created and validated two bioluminescent reporter strains for high-throughput screening, one in Pseudomonas aeruginosa, and another in a hyperporinated and efflux-deficient Escherichia coli.

Study on the Establishment Process of Muscle Synergy Based on Cosine Similarity.

Muscle synergy is an important method for motor intention recognition in rehabilitation exoskeleton control. The use of the non-negative matrix factorization (NMF) to extract muscle synergy patterns often results in long calculation time due to the amount of data, which makes the effectiveness of synergy extraction low. In this paper, synergy matrices of the complete single-cycle signal while stretching and its segmented ones were extracted respectively.