Silver Nanoparticle-Decorated Cellulose Nanocrystal Reinforced Ionic Polymer Hydrogel With High Conductivity and Environmental Tolerance for Multifunctional Sensing and Emergency Alarm System.

Conductive hydrogels hold great promise for flexible electronics. However, the simultaneous achievement of satisfactory mechanical strength, outstanding environmental tolerance, high sensitivity, and multiple sensing applications in a single conductive hydrogel remains a significant challenge. Herein, ionic polymer-based hydrogels with a double conductive network consisting of [2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (DMC), 2-hydroxyethyl acrylate (HEA) and silver nanoparticle decorated cellulose nanocrystal (CNC@Ag) are prepared by a facile one-pot method.

AO-DETR: Anti-Overlapping DETR for X-Ray Prohibited Items Detection.

Prohibited item detection in X-ray images is one of the most essential and highly effective methods widely employed in various security inspection scenarios. Considering the significant overlapping phenomenon in X-ray prohibited item images, we propose an anti-overlapping detection transformer (AO-DETR) based on one of the state-of-the-art (SOTA) general object detectors, DETR with improved denoising anchor boxes (DINO). Specifically, to address the feature coupling issue caused by overlapping phenomena, we introduce the category-specific one-to-one assignment (CSA) strategy to constrain category-specific object queries in predicting prohibited items of fixed categories, which can enhance their ability to extract features specific to prohibited items of a particular category from the overlapping foreground-background features.

Physicochemical Properties and Metal Ion-Binding Capacity of Thermal-Induced Antarctic Krill Protein Aggregates under Different pH Conditions.

Protein properties can be modified by thermal treatment at different pH values, resulting in the formation of protein aggregates with diverse morphologies and functionalities. This study investigated the morphology of aggregates of Antarctic krill protein (AKPS) formed by thermal treatment (90 °C, 15 min) at pH 2, 3, 4, and 6-12, characterized the different morphologies of AKPS, and determined the metal ion (Ca, Fe, and Zn)-binding capacities. Results showed that heat treatment with different pH values generated various AKPS with distinct morphology and metal ion-binding abilities.

CircYTHDF1/miR-19b-3p/YTHDF1 axis contributes to pregnancy-induced hypertension development by enhancing vascular endothelial cell injury.

Objective: The biological role of circ_0004858 (circYTHDF1) in pregnancy-induced hypertension (PIH) and the underlying mechanisms were unknown, and which were explored in this study.

Methods: ELISA was employed to detect the level of inflammatory cytokines and biochemical parameters; flow cytometry was employed to detect cell apoptosis; western blot and qRT-PCR were employed to examine expression level.

Results: The level of IL-1β, TNF-α, IL-6, TGF-β1, ET-1, and Ang-II were significantly elevated in the peripheral blood of PIH patients.

C-Reactive Protein Level as a Novel Serum Biomarker in Sarcopenia.

Background: The role of C-reactive protein (CRP), an inflammatory marker, in the development of sarcopenia remains uncertain.

Methods: This cross-sectional research involved the enrollment of 207 patients, classified into two groups: 74 patients with sarcopenia and 133 patients without sarcopenia. Clinical data of the participants, including hand grip strength, walking speed, appendicular lean mass (ALM), and calf circumference, were collected and recorded.

Analysis of Baseline Molecular Factors Associated With the Risk of Central Nervous System Progression Among Alectinib-Treated Patients With ALK-Positive NSCLC.

Introduction: Despite receiving alectinib therapy, patients with ALK-positive NSCLC remain at risk of central nervous system (CNS) progression. Our retrospective study aimed to identify baseline clinical and molecular factors associated with the risk of CNS progression in this patient subset.

Methods: We analyzed the clinical, molecular, and imaging data of 318 patients with ALK-positive advanced NSCLC who received alectinib as first-line (1L-alectinib) or second-line (2L-alectinib) therapy at baseline (1L, n = 183; 2L, n = 135) and at disease progression (1L, n = 80; 2L, n = 76).

A flexible plasmonic substrate for sensitive surface-enhanced Raman scattering-based detection of fentanyl.

In this work, we demonstrate a straightforward and versatile approach for fabricating flexible SERS substrates for highly sensitive fentanyl detection. Our design strategy integrates the synthesis of a yolk-shell structured plasmonic nanomaterial with a flexible cellulose substrate. The resulting SERS platform demonstrates excellent sensing capabilities, achieving a fentanyl detection limit as low as 4.

Video Head Impulse Test Coherence Predicts Vertigo Recovery in Sudden Sensorineural Hearing Loss With Vertigo.

Objectives: Labyrinthitis significantly reduces quality of life due to prolonged vestibular symptoms in patients experiencing sudden sensorineural hearing loss with vertigo (SSNHLV). This study employed a novel coherence analysis in the video head impulse test (vHIT) to explore vertigo outcomes in SSNHLV patients.

Methods: A retrospective review was conducted on 48 SSNHLV patients who completed high-dose steroid treatment between December 2016 and April 2023.

Separate anterior paraventricular thalamus projections differentially regulate sensory and affective aspects of pain.

The experience of pain is complex, involving both sensory and affective components, yet the underlying neural mechanisms remain elusive. Here, we show that formalin-induced pain behaviors coincide with increased responses in glutamatergic neurons within the anterior paraventricular nucleus of the thalamus (PVA). Furthermore, we describe non-overlapping subpopulations of PVAVgluT2 neurons involved in sensory and affective pain processing, whose activity varies across different pain states.

Site-Specific Histidine Aza-Michael Addition in Proteins Enabled by a Ferritin-Based Metalloenzyme.

Histidine modifications of proteins are broadly based on chemical methods triggering N-substitution reactions such as aza-Michael addition at histidine's moderately nucleophilic imidazole side chain. While recent studies have demonstrated chemoselective, histidine-specific modifications by further exploiting imidazole's electrophilic reactivity to overcome interference from the more nucleophilic lysine and cysteine, achieving site-specific histidine modifications remains a major challenge due to the absence of spatial control over chemical processes. Herein, through X-ray crystallography and cryo-electron microscopy structural studies, we describe the rational design of a nature-inspired, noncanonical amino-acid-incorporated, human ferritin-based metalloenzyme that is capable of introducing site-specific post-translational modifications (PTMs) to histidine in peptides and proteins.

Separation of Lung Cancer Cells From Mixed Cell Samples Using Aptamer-Modified Magnetic Beads and Permalloy Micromagnets.

This study involved the design and fabrication of a microfluidic chip integrated with permalloy micromagnets. The device was used with aptamer-modified magnetic beads (MBs) of various sizes to successfully separate lung cancer cells from a mixture of other cells. The overall separation efficiency was evaluated based on the ratios of cells in the different outlets and inlets of the chip.

Higher protein intake may benefit in patients with prolonged mechanical ventilation.

Background: Patients with prolonged mechanical ventilation (PMV) is usually associated with muscle wasting and diaphragm weakness, resulting in high medical costs and mortality. Adequate energy and protein intake were beneficial in sarcopenia patients. We aimed to investigate the impact of protein intake in weaning parameters in patients with PMV.

N-methyladenosine and its epitranscriptomic effects on hematopoietic stem cell regulation and leukemogenesis.

N6-methyladenosine (m6A) RNA modification orchestrates cellular epitranscriptome through tuning the homeostasis of transcript stability, translation efficiency, and the transcript affinity toward RNA-binding proteins (RBPs). An aberrant m6A deposition on RNA can lead toward oncogenic expression profile (mRNA), impaired mitochondrial metabolism (mtRNA), and translational suppression (rRNA) of tumor suppressor genes. In addition, non-coding RNAs (ncRNAs), such as X-inactive specific transcript (XIST), miRNAs, and α-ketoglutarate-centric metabolic transcripts are also regulated by the m6A epitranscriptome.

Structurally Diverse Duclauxins from a Coral-Derived sp. and Insight into Determining the Configuration at C-1 of Heptacyclic Duclauxins by H NMR.

A chemical investigation of the coral-derived fungus sp. TJ403-AL05 led to the isolation of 18 duclauxin analogues (-), 14 of which, taladuxins A-N (-), are new and consist of the first example of duclauxin fused with one 1,6-dioxaspiro[4.5]decan-2-one moiety (), as well as its biosynthetic product (), and 12 6/6/6/5/6/6/6 heptacyclic derivatives (-).

Network pharmacology and experimental verification unraveled the mechanism of Bailing Capsule against asthma.

Asthma is a serious public health challenge around the world. Recent studies into traditional Chinese medicine preparations for asthma have yielded promising findings regarding Bailing Capsule's potential in bronchial asthma prevention and treatment. This study aims to initially clarify the potential mechanism of Bailing Capsule in the treatment of asthma using network pharmacology and in vitro experimental approaches.

OsPIPK-FAB, A Negative Regulator in Rice Immunity Unveiled by OsMBL1 Inhibition.

Phosphatidylinositol signaling system plays a crucial role in plant physiology and development, phosphatidylinositol phosphate kinases (PIPKs) are one of the essential enzymes responsible for catalyzing the synthesis of phosphatidylinositol bisphosphate (PIP2) within this signaling pathway. However, its mechanism of signal transduction remains poorly exploited in plants. OsMBL1, a jacalin-related mannose-binding lectin in rice, plays a crucial role in plant defense mechanisms, acting as a key component of the pattern-triggered immunity (PTI) pathway.

Identification of powdery mildew resistance quantitative trait loci in melon and development of resistant near-isogenic lines through marker-assisted backcrossing.

Background: Melon (Cucumis melo L.), an important cucurbit crop, faces production limitations due to powdery mildew (PM). Developing resistant varieties offers a sustainable, genetics-based alternative to chemical treatments.

Ciliopathy organoid models: a comprehensive review.

Cilia are membrane-bound organelles found on the surface of most mammalian cell types and play numerous roles in human physiology and development, including osmo- and mechanosensation, as well as signal transduction. Ciliopathies are a large group of, usually rare, genetic disorders resulting from abnormal ciliary structure or ciliary dysfunction that have a high collective prevalence. Autosomal dominant or recessive polycystic kidney disease (ADPKD/ARPKD), Bardet-Biedl-Syndrome, and primary ciliary dyskinesia (PCD) are the most frequent etiologies.

Elucidating the intrinsic relationship between redox properties of CeO2 and CH4 oxidation activity: A theoretical perspective.

Methane (CH4) oxidation is an important reaction to reduce the greenhouse effect caused by incomplete combustion of CH4. Here, we explored the mechanism of CH4 oxidation catalyzed by CeO2 and Ni-doped CeO2, focusing on the redox properties of these catalyst surfaces, using density functional theory (DFT). We found that the barriers for CH4* activation and H2O* formation are correlated with the surface redox capacity, which is enhanced by Ni doping.

Features and Differences in Core Symptom Clusters in Home-Based Hospice Patients With Advanced Cancer: A Network Analysis.

Introduction: Patients with terminal-stage cancer frequently experience multiple symptoms simultaneously. Little is known about how core symptom clusters differ in advanced-cancer patients with different survival expectancies receiving hospice care. To identify the core symptom clusters of hospice-care cancer patients with different survival expectancies and compare the features of their symptom networks.