Tylvalosin exhibits anti-inflammatory property and attenuates acute lung injury in different models possibly through suppression of NF-κB activation.

Tylvalosin, a new broad-spectrum, third-generation macrolides, may exert a variety of pharmacological activities. Here, we report on its anti-oxidative and anti-inflammatory activity in RAW 264.7 macrophages and mouse treated with lipopolysaccharide (LPS) as well as piglet challenged with porcine reproductive and respiratory syndrome virus (PRRSV).

Preparation and evaluation of dl-praeruptorin A microemulsion based hydrogel for dermal delivery.

The purpose of the present investigation was to develop and optimize the microemulsion (ME) as a transdermal system for Pd-Ia, a poor water soluble and low bioavailable drug. The pseudo-ternary phase diagrams were constructed for various ME formulations including oleic acid as the oil phase, Cremophor RH40 as the surfactant, ethanol as the cosurfactant, and water. The maximum cumulative amount permeated through rat abdominal skins per unit area in 32 h (Q32), and the maximum flux were evaluated using the Franz diffusion cell in order to optimize the ME formulation.

Remarkably enhanced photocatalytic activity of ordered mesoporous carbon/g-C₃N₄ composite photocatalysts under visible light.

Ordered mesoporous carbon/g-C3N4 (OMC/g-C3N4) composites with efficient photocatalytic activity under visible light irradiation were prepared by a facile heating method. The as-prepared OMC/g-C3N4 composites were thoroughly characterized by X-ray diffraction, Fourier transform infrared spectroscopy, elemental analyses, transmission electron microscopy with energy dispersion X-ray spectroscopy, N2 adsorption-desorption analysis, UV-vis diffuse reflectance spectroscopy and photoluminescence spectroscopy. The photocatalytic activities were evaluated by degrading Rhodamine B dye, and OMC/g-C3N4 composites exhibited much higher photocatalytic activities than pristine g-C3N4.

A simple and reliable sensor for accurate measurement of angular speed for low speed rotating machinery.

This paper presents the theory and implementation of a novel sensor system for measuring the angular speed (AS) of a shaft rotating at a very low speed range, nearly zero speed. The sensor system consists mainly of an eccentric sleeve rotating with the shaft on which the angular speed to be measured, and an eddy current displacement sensor to obtain the profile of the sleeve for AS calculation. When the shaft rotates at constant speed the profile will be a pure sinusoidal trace.

Overexpression of TRIM24 is associated with the onset and progress of human hepatocellular carcinoma.

The survival and colonization of tumor cells at new locations involve a variety of complex genetic, epigenetic, and microenvironmental factors. TRIM24 was originally named transcription intermediary factor 1-alpha (TIF1α), which was associated with cellular proliferation and was an oncogene in tumor development. Here we provide the first evidence of the expression profile and clinicopathological significance of TRIM24 in patients with hepatocellular carcinoma (HCC).

Chromosome-8-coded proteome of Chinese Chromosome Proteome Data set (CCPD) 2.0 with partial immunohistochemical verifications.

We upgraded the preliminary CCPD 1.0 to CCPD 2.0 using the latest deep-profiling proteome (CCPD 2013) of three hepatocellular carcinoma (HCC) cell lines, namely, Hep3B, MHCC97H, and HCCLM3 (ProteomeXchange identifiers: PXD000529, PXD000533, and PXD000535).

Omics evidence: single nucleotide variants transmissions on chromosome 20 in liver cancer cell lines.

Cancer genomics unveils many cancer-related mutations, including some chromosome 20 (Chr.20) genes. The mutated messages have been found in the corresponding mRNAs; however, whether they could be translated to proteins still requires more evidence.

Systematic analysis of missing proteins provides clues to help define all of the protein-coding genes on human chromosome 1.

Our first proteomic exploration of human chromosome 1 began in 2012 (CCPD 1.0), and the genome-wide characterization of the human proteome through public resources revealed that 32-39% of proteins on chromosome 1 remain unidentified. To characterize all of the missing proteins, we applied an OMICS-integrated analysis of three human liver cell lines (Hep3B, MHCC97H, and HCCLM3) using mRNA and ribosome nascent-chain complex-bound mRNA deep sequencing and proteome profiling, contributing mass spectrometric evidence of 60 additional chromosome 1 gene products.

Systematic analyses of the transcriptome, translatome, and proteome provide a global view and potential strategy for the C-HPP.

To estimate the potential of the state-of-the-art proteomics technologies on full coverage of the encoding gene products, the Chinese Human Chromosome Proteome Consortium (CCPC) applied a multiomics strategy to systematically analyze the transciptome, translatome, and proteome of the same cultured hepatoma cells with varied metastatic potential qualitatively and quantitatively. The results provide a global view of gene expression profiles. The 9064 identified high confident proteins covered 50.

[Relationship between the expressions of indoleamine 2, 3-dioxygenase in hepatocellular carcinoma and clinicopathological parameters].

Objective: To explore the expressions of indoleamine 2, 3-dioxygenase (IDO) in hepatocellular carcinoma and analyze its relationship with clinicopathological parameters.

Methods: Quantitative real-time polymerase chain reaction (PCR), fluorogenic quantitative PCR, immunohistochemical and immunofluorescence were used to detect the expression of indoleamine 2, 3-dioxygenase in hepatocellular carcinoma.

Results: The IDO mRNA expression in cancerous tissues increased markedly than that in the corresponding non-cancerous tissues (2(-ΔΔCT) = 1.

Contextualized trajectory parsing with spatiotemporal graph.

This work investigates how to automatically parse object trajectories in surveillance videos, which aims at jointly solving three subproblems: 1) spatial segmentation, 2) temporal tracking, and 3) object categorization. We present a novel representation spatiotemporal graph (ST-Graph) in which: 1) Graph nodes express the motion primitives, each representing a short sequence of small-size patches over consecutive images, and 2) every two neighbor nodes are linked with either a positive edge or a negative edge to describe their collaborative or exclusive relationship of belonging to the same object trajectory. Phrasing the trajectory parsing as a graph multicoloring problem, we propose a unified probabilistic formulation to integrate various types of context knowledge as informative priors.

Quantitative evaluation of the mitochondrial proteomes of Drosophila melanogaster adapted to extreme oxygen conditions.

Mitochondria are the primary organelles that consume oxygen and provide energy for cellular activities. To investigate the mitochondrial mechanisms underlying adaptation to extreme oxygen conditions, we generated Drosophila strains that could survive in low- or high-oxygen environments (LOF or HOF, respectively), examined their mitochondria at the ultrastructural level via transmission electron microscopy, studied the activity of their respiratory chain complexes, and quantitatively analyzed the protein abundance responses of the mitochondrial proteomes using Isobaric tag for relative and absolute quantitation (iTRAQ). A total of 718 proteins were identified with high confidence, and 55 and 75 mitochondrial proteins displayed significant differences in abundance in LOF and HOF, respectively, compared with the control flies.

[Surgical techniques and perioperative management for improving the success rate of orthotopic liver transplantation in rats].

Objective: To investigate the surgical techniques and appropriate perioperative management for ensuring successful orthotopic liver transplantation (ROLT) in rats.

Methods: Based on the double-cuff technique of Kamada, we modified the surgical techniques of separation, perfusion and cold preservation of the donor liver, shearing and anastomosis of the suprahepatic vena cava with optimized postoperative infusion protocols and animal care.

Results: Two hundred and seventy rats underwent ROLT and a learning curve of the success rate was built to reflect the improvement of techniques.

Stress responsive proteins are actively regulated during rice (Oryza sativa) embryogenesis as indicated by quantitative proteomics analysis.

Embryogenesis is the initial step in a plant's life, and the molecular changes that occur during embryonic development are largely unknown. To explore the relevant molecular events, we used the isobaric tags for relative and absolute quantification (iTRAQ) coupled with the shotgun proteomics technique (iTRAQ/Shotgun) to study the proteomic changes of rice embryos during embryogenesis. For the first time, a total of 2 165 unique proteins were identified in rice embryos, and the abundances of 867 proteins were actively changed based on the statistical evaluation of the quantitative MS/MS signals.

Dissipation and residues of clethodim and its oxidation metabolites in a rape-field ecosystem using QuEChERS and liquid chromatography/tandem mass spectrometry.

A rapid, sensitive and selective method using Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) procedure for simultaneous determination of clethodim and its oxidation metabolites (clethodim sulfoxide and clethodim sulphone) in soil, rape plant and rape seed was developed using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). The limits of detection (LODs) of the proposed method ranged from 0.002mg/kg to 0.

BMP-6 inhibits cell proliferation by targeting microRNA-192 in breast cancer.

Although bone morphogenetic protein-6 (BMP-6) has been identified as a tumor suppressor associated with breast cancer differentiation and metastasis, the potential roles of BMP-6 in regulating cell cycle progression have not been fully examined. In the present study, we provide the novel finding that induction of BMP-6 in MDA-MB-231 breast cancer cells significantly inhibits cell proliferation by decreasing the number of cells in S phase of the cell cycle, resulting in inhibition of tumorigenesis in a nude mouse xenograft model. Further investigation indicated that BMP-6 up-regulates the expression of microRNA-192 (miR-192) in MDA-MB-231 cells.

[Relation of B7-H3 molecule expression in multiple myeloma with poor prognosis and bone destruction].

This study was purpose to investigate the B7-H3 expression in multiple myeloma cell lines and CD138 cells of patients with multiple myeloma, and explore its clinical significance. Three myeloma cell lines (RPMI8226, U266 and H929) were used. Forty-five patients with multiple myeloma were enrolled in the study.

[Expression of Nusap1 in the surgical margins of hepatocellular carcinoma and its association with early recurrence].

Objective: To detect the expression of Nusap1 of surgical margins in hepatocellular carcinoma (HCC) and investigate its association with early tumor recurrence.

Methods: The expression of Nusap1 in the surgical margins of HCC, which were histopathologically negative for tumor cells, was examined using immunohistochemistry in 61 HCC cases.

Results: Fifteen of 21 (71.

High-performance flexible electrochromic device based on facile semiconductor-to-metal transition realized by WO3·2H2O ultrathin nanosheets.

Ultrathin nanosheets are considered as one kind of the most promising candidates for the fabrication of flexible electrochromic devices (ECDs) due to their permeable channels, high specific surface areas, and good contact with the substrate. Herein, we first report the synthesis of large-area nanosheets of tungsten oxide dihydrate (WO3·2H2O) with a thickness of only about 1.4 nm, showing much higher Li(+) diffusion coefficients than those of the bulk counterpart.

Quantitative proteomics reveals the temperature-dependent proteins encoded by a series of cluster genes in thermoanaerobacter tengcongensis.

Comprehensive and quantitative information of the thermophile proteome is an important source for understanding of the survival mechanism under high growth temperature. Thermoanaerobacter tengcongensis (T. tengcongensis), a typical anaerobic thermophilic eubacterium, was selected to quantitatively evaluate its protein abundance changes in response to four different temperatures.

Quantitative analysis of the human AKR family members in cancer cell lines using the mTRAQ/MRM approach.

Members of human aldo-keto reductase (AKR) superfamily have been reported to be involved in cancer progression, whereas the final conclusion is not generally accepted. Herein, we propose a quantitative method to measure human AKR proteins in cells using mTRAQ-based multiple reaction monitoring (MRM). AKR peptides with multiple transitions were carefully selected upon tryptic digestion of the recombinant AKR proteins, while AKR proteins were identified by SDS-PAGE fractionation coupled with LC-MS/MS.