Publications by authors named "Liming Hou"

The volume-regulated anion channel (VRAC) plays a critical role in cell volume regulation and other fundamental physiological processes. However, the mechanism of how VRAC is activated and modulated has not been completely clarified. Caveolin-1 (Cav-1), as an important ion channel binding protein, forms complexes with channel proteins and exchangers to regulate channel activity and function.

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Unlabelled: Although metagenomic investigations into microbial fiber-degrading capabilities are currently prevalent, there is a notable gap in research concerning the regulatory mechanisms underpinning host-microbiota interactions that confer tolerance to high-fiber diets in pigs. In this study, 28 Meishan (MS) and 28 Large White (LW) pigs were subjected to feeding experiments involving various fiber levels. Subsequently, multi-omics was employed to investigate the influence of host-microbiota interactions on the fiber degradation of pigs.

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The bone marrow (BM) niche is critical in regulating hematopoiesis, and sexual dimorphism and its underlying mechanism in the BM niche and its impact on hematopoiesis are not well understood. We show that male mice exhibited a higher abundance of leptin-receptor-expressing mesenchymal stromal cells (LepR-MSCs) compared with female mice. Sex-mismatched coculture and BM transplantation showed that the male BM niche provided superior support for in vitro colony formation and in vivo hematopoietic engraftment.

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Atherosclerosis (AS) is a chronic disease initiated by vascular endothelial dysfunction, with low shear stress (SS) being a critical inducing factor in this dysfunction. Apoptosis, a form of programmed cell death, is closely associated with AS progression. However, the impact of low SS on endothelial apoptosis and its specific molecular mechanisms remains unclear.

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  • The study looks at how four important knee ligaments (ACL, PCL, MCL, LCL) react to sudden forces while the person is standing still.
  • Researchers created a 3D computer model of the knee from MRI scans and tested how different loads affect the ligaments in various parts of the knee.
  • Results showed that the PCL experienced the most stress under both front and back loads, while the ACL and LCL had the highest stress during side impacts.
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BACKGROUNDFrailty significantly affects morbidity and mortality rates in the older population (age >65 years). Age-related degenerative diseases are influenced by the intestinal microbiota. However, limited research exists on alterations in the intestinal microbiota in frail older individuals, and the effectiveness of prebiotic intervention for treating frailty remains uncertain.

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  • Older individuals with glucose metabolic disorders, including prediabetes and diabetes, have a higher risk of developing heart failure compared to those with normal glucose levels.
  • The study assessed over 13,000 participants, measuring various adiposity metrics like BMI, waist-to-hip ratio, and visceral fat, finding that central obesity significantly impacts heart failure risk.
  • Results revealed that waist-to-hip ratio particularly indicated elevated heart failure risk in participants with glucose issues, with a notable population attributable risk percentage linked to central obesity across different glucose categories.
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In this study, we used 16S high-throughput sequencing to investigate the effects of uranium mining on the rhizospheric bacterial communities and functions of three local plant species, namely, Artemisia frigida, Acorus tatarionwii Schott., and Salix oritrepha Schneid. The results showed that uranium mining significantly reduced the diversity of rhizospheric bacteria in the three local plant species, including the Shannon index and Simpson index (P < 0.

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Quinoa is a nutrient-dense pseudocereal that has garnered global attention for its potential to bolster food security and nutrition. Despite its celebrated status, the detailed nutritional profiles of various quinoa varieties remain poorly understood, which poses a significant barrier to the strategic cultivation and utilization of quinoa's genetic diversity to combat malnutrition. The impetus for this research lies in the urgent need to identify superior quinoa strains that can be tailored to meet specific nutritional requirements and adapt to diverse agro-ecological zones.

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  • A low level of drip loss (DL) is crucial for high-quality meat, but the specific genes involved in DL for pork are largely unknown.
  • Researchers analyzed twelve pigs with differing DL levels using transcriptome sequencing and various analytical methods to identify important genes affecting DL.
  • They identified nine key genes linked to important signaling pathways and functions, providing insights into improving meat quality through molecular breeding efforts.
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  • Muscle satellite cells (MuSCs) are essential for muscle growth and repair, and primary pig MuSCs (pMuSCs) serve as a key model for research in pig muscle development.
  • Long-term culture of pMuSCs loses their stemness, which hampers research; to solve this, scientists created immortalized pMuSCs (SV40 T-pMuSCs) using a lentiviral vector that allows them to be cultured for over 40 generations.
  • SV40 T-pMuSCs show similar growth to primary cells initially but have slightly reduced differentiation potential, which can be improved by silencing SV40 T-antigen expression, making them a valuable resource for studying pig skeletal muscle.
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  • The study focused on discovering genetic markers and candidate genes linked to digestive traits in pigs by analyzing 331 pigs using genetic data from either 80K or 50K Chip technology.
  • Two different approaches revealed multiple single nucleotide polymorphisms (SNPs) associated with fiber digestibility, identifying three quantitative trait locus (QTL) regions for both apparent neutral detergent and acid detergent fiber digestibility.
  • Additionally, two genes, MST1 and LATS1, were found near significant SNPs, linked to intestinal health, laying a foundation for future research on pig digestive traits.
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Intramuscular fat content (IFC) and meat color are vital indicators of pork quality. A significant positive correlation between IFC and redness of meat color (CIE value) indicates that these two traits are likely to be regulated by shared molecular pathways.To identify candidate genes, hub genes, and signaling pathways that regulate these two traits, we measured the IFC and CIE value in 147 hybrid pigs, and selected individuls with extreme phenotypes for transcriptome analysis.

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The introduction of single-cell RNA sequencing (scRNA-seq) technology has spurred additional advancements in analyzing the cellular composition of tissues. The (LD) in pigs serves as the primary skeletal muscle for studying meat quality in the pig industry. However, the single-cell profile of porcine LD is still in its infancy stage.

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  • Eurasian pigs have experienced genetic mixing over time, particularly with indigenous breeds from China like the Meishan and Erhualian pigs improving Western breeds such as the French Large White pigs in reproductive performance.
  • This research identified that Danish Large White pigs have also incorporated genetic material from Taihu Lake pigs, specifically showing shared haplotypes that are linked to neurodevelopmental pathways.
  • A specific 115 kb genomic region on chromosome 16 contains a key gene, NDUFS4, which may significantly enhance reproductive performance, indicating its potential as a target for breeding improvements in pigs.
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To evaluate the tolerance of a high-fiber diet in Erhualian pigs (Er-HL), the present investigation systematically investigated the ramifications of varying wheat bran fiber levels, specified as total dietary fiber (TDF) values of 14.07%, 16.32%, 17.

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The high-fertility Meishan pig is currently categorized into medium sized (MMS) and small sized (SMS) based on body size. To identify causal genes responsible for the variation in body size within the two categories, we sequenced individuals representing the entire consanguinity of the existing Meishan pig. This enabled us to conduct genome selective signal analysis.

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Background: The impacts and mechanisms of morning hypertension (MHT) on the risk of new-onset atrial fibrillation (AF) in the elderly have not been clarified. We aimed to investigate an association between MHT and new-onset AF and explore a mediating effect of subclinical inflammation on this association.

Methods: From 2008 to 2010, 1789 older adults aged ≥60 years were recruited in Shandong area, China.

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Zearalenone (ZEA) is a prevalent mycotoxin functions as an endocrine disrupter to the reproductive systems of farm animals, especially in pigs. To evaluate the effect and the underlying molecular changes that occurred when the porcine germline stem cells were exposed to ZEA, prospermatogonia (ProSGs) were enriched and treated with a gradient concentration (0-10 μM) of ZEA for 2-8 days. Our results showed that the ZEA treatment inhibited the proliferation of ProSGs in a dose-dependent manner with a critical concentration at 1 μM.

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Purpose: Fall is a common geriatric syndrome leading to various adverse outcomes in the elderly. Gait and balance disorders and decreased lower extremity muscle function are the major intrinsic risk factors of falls, and studies suggested that they were closely related to the underlying chronic conditions. This study aimed to explore the patterns of multimorbidity and determine the associations of these multimorbidity patterns with gait, balance and lower extremity muscle function.

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  • * There is a noted connection between an imbalance in gut flora and lung cancer, though the exact mechanisms remain unclear.
  • * The study compares Eastern and Western medicine approaches, highlighting how traditional Chinese medicine ingredients can potentially regulate gut flora to aid in preventing and treating NSCLC.
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The number of ribs (NR) and carcass length (CL) are important economic traits in pig breeding programs. Pigs with a higher NR and longer CL produce greater pork yields. In the present study, Suhuai pigs with NR and CL phenotypes were genotyped using the Neogen GGP Porcine 80 K SNP array to identify the QTL affecting NR and CL and dissect the candidate genes for the two traits.

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Meat color is an attractive trait that influences consumers' purchase decisions at the point of sale. To decipher the genetic basis of meat color traits, we performed a genome-wide association study based on low-coverage whole-genome sequencing. In total, 669 (Pietrain × Duroc) × (Landrace × Yorkshire) pigs were genotyped using low-coverage whole-genome sequencing.

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  • Integrating single-nucleotide polymorphisms (SNPs) from whole-genome sequencing (iWGS) into genomic prediction (GP) models can enhance prediction accuracy for traits like intramuscular fat content (IFC) in pigs.
  • The study analyzed 482 Suhuai pigs, identifying significant regions and functional genes related to IFC through genome-wide association studies (GWAS) and various GP models, including pedigree-based BLUP (PBLUP) and different GBLUP approaches.
  • Findings showed that incorporating iWGS SNPs improved prediction reliability, with the best results seen in the two-component GBLUP and BayesMix models, highlighting the potential for further gene identification related to IFC.
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Previous study found that appropriate high-fiber diet (containing 19.10% total dietary fiber [TDF], treatment II) did not reduce apparent fiber digestibility of Chinese Suhuai finishing pigs and increased the yield of short-chain fatty acids (SCFAs), but too high-fiber diet (containing 24.11% TDF, treatment IV) significantly reduced apparent fiber digestibility compared with normal diet (containing 16.

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