Pyrotinib plus capecitabine for patients with HER2-positive metastatic breast cancer and brain metastases (PERMEATE trial): overall survival results from a multicenter, single-arm, two-cohort, phase 2 trial.

Background: The phase 2 PERMEATE study has shown the antitumor activity and safety of pyrotinib plus capecitabine in patients with human epidermal growth factor receptor 2 (HER2)-positive metastatic breast cancer and brain metastases. In this report, survival results were updated with extended follow-up.

Methods: Between January 29, 2019 and July 10, 2020, adult patients with HER2-positive metastatic breast cancer who had radiotherapy-naïve brain metastases (cohort A, n = 59) or progressive disease after radiotherapy (cohort B, n = 19) were enrolled and received pyrotinib (400 mg once daily) and capecitabine (1000 mg/m twice daily on days 1-14 of each 21-day cycle) until disease progression or unacceptable toxicity.

Dalpiciclib and pyrotinib in women with HER2-positive advanced breast cancer: a single-arm phase II trial.

CDK4/6 inhibitors have shown a synergistic effect with anti-HER2 therapy in hormone receptor (HR)-positive and HER2-positive breast cancer (BC). In this phase 2 study (NCT04293276), we aim to evaluate a dual-oral regimen of CDK4/6 inhibitor dalpiciclib combined with HER2 tyrosine kinase inhibitor pyrotinib as front-line treatment in women with HER2-positive advanced BC (n = 41) including those with HR-negative disease. The primary endpoint is the objective response rate, and secondary endpoints include progression-free survival (PFS), overall survival (OS), and safety.

Temporal patterns of algae in different urban lakes and their correlations with environmental variables in Xi'an, China.

Urban lakes were critical in aquatic ecology environments, but how environmental factors affected the distribution and change characteristics of algal communities in urban lakes of Xi'an city was not clearly. Here, we investigated the algal community structure of six urban lakes in Xi'an and evaluated the effects of water quality parameters on algae. The results indicated that the significant differences on physicochemical parameters existed in different urban lakes.

Promoted aerobic denitrification through denitrifying fungal communities: Co-occurrence patterns and treatment of low C/N micro-polluted water.

Despite the growing interest in using mixed-culture aerobic denitrifying fungal flora (mixed-CADFF) for water remediation, there is limited research on their nitrogen removal performance in low C/N polluted water bodies. To address this knowledge gap, we isolated three mixed-CADFFs from overlying water in urban lakes to evaluate their removal performance. The total nitrogen (TN) removal efficiencies were 93.

Effects of copper sulfate algaecide on the cell growth, physiological characteristics, the metabolic activity of Microcystis aeruginosa and raw water application.

Harmful cyanobacteria blooms (HCBs) occurred frequently and become a serious scientific challenge. Copper sulfate (CuSO) is a broad-spectrum chemical algaecide to control algae blooms. Herein, the Microcystis aeruginosa was exposed to different CuSO (0.

Clinicopathological features and prognosis of breast cancer combined with symptomatic bone marrow metastases: A 10-year, single-center, real-world study of 67 cases.

Purpose: Bone marrow metastasis (BMM) is uncommon in breast cancer (BC), and early diagnosis is challenging. BMM lacks definitive treatment options and poses a great threat to the survival of patients. Herein, we investigated the clinical features, prognosis, and factors affecting the prognosis of BC patients with symptomatic BMM to help improve the understanding of this disease and provide effective diagnostic and treatment strategies.

Aerobic Denitrification Enhanced by Immobilized Slow-Released Iron/Activated Carbon Aquagel Treatment of Low C/N Micropolluted Water: Denitrification Performance, Denitrifying Bacterial Community Co-occurrence, and Implications.

The key limiting factors in the treatment of low C/N micropolluted water bodies are deficient essential electron donors for nitrogen removal processes. An iron/activated carbon aquagel (IACA) was synthesized as a slowly released inorganic electron donor to enhance aerobic denitrification performance in low C/N micropolluted water treatment. The denitrification efficiency in IACA reactors was enhanced by more than 56.

Nitrogen reduction by aerobic denitrifying fungi isolated from reservoirs using biodegradation materials for electron donor: Capability and adaptability in the lower C/N raw water treatment.

Biological denitrification was considered an efficient and environmentally friendly way to remove the nitrogen in the water body. However, biological denitrification showed poor nitrogen removal performance due to the lack of electron donors in the low C/N water. In this study, three novel aerobic denitrifying fungi (Trichoderma sp.

Novel insights into aerobic denitrifying bacterial communities augmented denitrification capacity and mechanisms in lake waters.

Scanty attention has been paid to augmenting the denitrification performance of polluted lake water by adding mix-cultured aerobic denitrifying bacterial communities (Mix-CADBCs). In this study, to solve the serious problem of nitrogen pollution in lake water bodies, aerobic denitrifying bacteria were added to lake water to enhance the nitrogen and carbon removal ability. Three Mix-CADBCs were isolated from lake water and they could remove >94 % of total nitrogen and dissolved organic carbon, respectively.

Aerobic denitrifying using actinobacterial consortium: Novel denitrifying microbe and its application.

The aerobic denitrifying capacity of actinomycete strain has been investigated recently, while little is known about nitrogen and carbon substrate removal by mix-cultured aerobic denitrifying actinobacteria (Mix-CADA) community. Hence, three Mix-CADA consortiums, named Y23, X21, and Y27, were isolated from urban lakes to investigate their aerobic denitrification capacity, and their removal efficiency for nitrate and dissolved organic carbon were >97 % and 90 %, respectively. Illumina Miseq sequencing revealed that Streptomyces was the most dominant genus in the Mix-CADA consortium.

Characterization of non-taste & odor produced aerobic denitrification actinomycetes strains Streptomyces spp. isolated from reservoir ecosystem: Denitrification performance and carbon source metabolism.

The aerobic denitrification performance of actinomycetes was investigated. Two strains of actinomycetes were isolated and identified as Streptomyces sp. LJH-12-1 and Streptomyces diastatochromogenes LJH-12-2.

Diagnostic diversity and heterogeneity of tumors: a real-world study of metastasis re-biopsy in advanced breast cancer.

Background: Re-biopsy of metastasis in advanced breast cancer (ABC) has become an international convention to assist the diagnosis and evaluation of tumor heterogeneity. This study aimed to detect diagnostic diversity and inconsistencies among estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) expression levels between primary and metastatic lesions.

Methods: We conducted a retrospective analysis of 1670 cases of ABC patients who had undergone at least one lesion re-biopsy from January 2010 to December 2018.

Surgical Efficacy and Prognosis of 54 Cases of Spinal Metastases from Breast Cancer.

Objective: To analyze the efficacy and complications of spinal metastasis surgery for breast cancer; to understand the survival and the influencing factors; and to verify the predictive ability of the currently used spinal metastasis cancer survival prediction scoring system on 1 year postoperative survival.

Methods: A retrospective study was conducted of 54 patients with spinal metastases from breast cancer who underwent open surgery after multidisciplinary consultation in our hospital from January 2017 to October 2020. Patient demographic-related variables, breast cancer-related variables, spinal disorder-related variables, and treatment-related variables were collected.

Mix-cultured aerobic denitrifying bacteria augmented carbon and nitrogen removal for micro-polluted water: Metabolic activity, coexistence and interactions, and immobilized bacteria for reservoir raw water treatment.

Less attention has been paid on the oligotrophic water body nitrogen treatment with mix-cultured aerobic denitrifying bacteria (Mix-CADB). In this study, three Mix-CADB communities were screened from the sediments of reservoirs. The nitrate and dissolved organic carbon (DOC) removal efficiencies of Mix-CADB communities were higher than 92 % and 91 %, respectively.

Cooperation triggers nitrogen removal and algal inhibition by actinomycetes during landscape water treatment: Performance and metabolic activity.

The actinomycetes strain Streptomyces sp. XD-11-9-3 and Streptomyces sp. 5 were isolated and presented poor denitrification performance.

Nitrogen removal from low carbon/nitrogen polluted water is enhanced by a novel synthetic micro-ecosystem under aerobic conditions: Novel insight into abundance of denitrification genes and community interactions.

The main limiting factor in treatment of wastewater with a low carbon/nitrogen ratio is insufficient electron donors for aerobic denitrification. A novel synthetic micro-ecosystem (SM) with functional materials as the core structure was prepared to enhance nitrate removal during wastewater treatment. Nitrate removal in the reactors with SM increased by more than 40 % and reached 97.

Pyrotinib plus capecitabine for patients with human epidermal growth factor receptor 2-positive breast cancer and brain metastases (PERMEATE): a multicentre, single-arm, two-cohort, phase 2 trial.

Background: Patients with HER2-positive metastatic breast cancer have a high risk of developing brain metastases. Efficacious treatment options are scarce. We investigated the activity and safety of pyrotinib plus capecitabine in patients with HER2-positive metastatic breast cancer and brain metastases.

Serum exosomal miR-377-3p and miR-381-3p as diagnostic biomarkers in colorectal cancer.

This study aimed to identify specific and sensitive exosomal miRNAs in diagnosing patients with colorectal cancer (CRC). Serum exosomes were isolated from 175 CRC patients and 172 healthy donors by ultracentrifugation and identified by transmission electron microscopy, nanoparticle tracking analysis and western blotting. Exosomal miRNA expression was detected by quantitative PCR and the results analyzed by receiver operating characteristic analysis to illuminate the diagnostic accuracy.

Efficacy and safety of bevacizumab-containing therapy for refractory advanced breast cancer: a retrospective study.

Background: Advanced breast cancer (ABC) is difficult to treat due to the primary and acquired resistance, which is the main cause of rescue treatment failure. Thus, developing a new rescue treatment strategy for clinicians is a challenge. This study retrospectively collected the medical information of patients with refractory ABC who were treated with bevacizumab at our department to analyze the efficacy and safety of bevacizumab-containing therapy as a new treatment method for refractory ABC.

Clinical diagnosis and treatment of breast cancer with brain metastases and establishment of a prognostic model: a 10-year, single-center, real-world study of 559 cases.

Background: This study involved a retrospective analysis of 559 metastatic breast cancer (MBC) patients with brain metastasis (BM). We aimed to establish the effectiveness of different preferred treatment methods and factors affecting overall survival following BM diagnosis (BMOS) and explore the feasibility of systemic treatment for MBC patients with BM.

Methods: Univariate and multivariate analyses were used to assess the efficacy of different preferred treatments and other factors associated with BMOS, and a nomogram was then established based on the results of the univariate analysis.

Distress Thermometer in breast cancer: systematic review and meta-analysis.

Objective: This meta-analysis aimed to reach a summarised estimate of distress prevalence screened by Distress Thermometer (DT) among patients with breast cancer and compare different pooled prevalence estimated between different subgroups.

Methods: Two independent interviewers conducted a systematic search from PubMed, EMBASE, Ovid and Cochrane Library and checked related reviews and meta-analyses for eligible studies. The studies that identified distress of patients with breast cancer with DT were included.

TEP linc-GTF2H2-1, RP3-466P17.2, and lnc-ST8SIA4-12 as novel biomarkers for lung cancer diagnosis and progression prediction.

Purpose: Platelets contain a rich repertoire of RNA species, such as mRNAs and long non-coding RNAs. During the development of tumors, platelets are "educated" by cancer cells, altering their transcriptome and molecular content, thereby, tumor educated platelet (TEP) lncRNA profile has the potential to diagnose lung cancer. The current study was aimed to examine whether TEPs might be a potential biomarker for lung cancer.