Nanolevel of detection of ascorbic acid using horse-radish peroxidase inhibition assay.

Ascorbic acid plays a significant role in regulation of various bodily functions with high concentrations in immune cells and being involved in connective tissue maintenance. Commonly it is detected through various colorimetric methods. In this study, we propose a one-step simple method based on the inhibitory activity of ascorbic acid on horseradish peroxidase and hydrogen peroxide.

Isolation of anticancer bioactive secondary metabolites from the sponge-derived endophytic fungi . and computational docking approach.

Introduction: Fungus-derived secondary metabolites are fascinating with biomedical potential and chemical diversity. Mining endophytic fungi for drug candidates is an ongoing process in the field of drug discovery and medicinal chemistry. Endophytic fungal symbionts from terrestrial plants, marine flora, and fauna tend to produce interesting types of secondary metabolites with biomedical importance of anticancer, antiviral, and anti-tuberculosis properties.

New Alkaloids and Polyketides from the Marine Sponge-Derived Fungus sp. SCSIO41015.

The sponge-derived fungus sp. SCSIO41015 cultured on solid rice medium yielded twenty-one compounds (-), including two new alkaloids ( and ) and one new pyrone derivative (). Their structures were elucidated by analysis of 1D/2D NMR data and HR-ESI-MS.

Cytotoxicity of polyketides and steroids isolated from the sponge-associated fungus SCSIO 41017.

Two new polyketides, xerucitrinin A () and coniochaetone M (), and one new steroid, 16-methylpregna-17,19-dihydroxy-(9,11)-epoxy-4-ene-3,18-dione-20-acetoxy (), together with eleven known analogues were isolated from fungus SCSIO 41017 associated with the sponge sp. Their structures and absolute configurations were elucidated by NMR spectra, MS, CD, optical rotation, X-ray crystallography, and compared with literature data. Biological evaluation results revealed that exhibited significant cytotoxic activity against MCF-7 cell line with IC values of 1.

Versispiroketal A, an unusual tetracyclic bridged spiroketal from the sponge-associated fungus Aspergillus versicolor SCSIO 41013.

Versispiroketal A (1), an unprecedented 6/5/5/6 tetracyclic polyketide featuring a rarely encountered bridge-fused spiroketal skeleton, was isolated from the sponge-associated fungus Aspergillus versicolor SCSIO 41013. The structure and absolute configuration of 1 were unequivocally determined by comprehensive spectroscopic analysis, single-crystal X-ray diffraction analysis and quantum chemical ECD calculations. Compound 1 showed weak cytotoxicity against four cancer cell lines.

Bioactive Novel Indole Alkaloids and Steroids from Deep Sea-Derived Fungus SCSIO 41012.

Two new alkaloids, fumigatosides E () and F (), and a new natural product, 3, 7-diketo-cephalosporin P₁ () along with five known compounds (⁻, , ) were isolated from deep-sea derived fungal SCSIO 41012. Their structures were determined by extensive spectroscopic data analysis, including 1D, 2D nuclear magnetic resonance (NMR) and mass spectrometry (MS), and comparison between the calculated and experimental electronic circular dichroism (ECD) spectra. In addition, all compounds were tested for antibacterial and antifungal inhibitory activities.

Peptides from the Soft Coral-associated Fungus Simplicillium sp. SCSIO41209.

Five new peptides, Sinulariapeptides A-E together with seven known peptides (6-12) were isolated from the soft coral associated fungus Simplicillium sp. SCSIO 41209. The structures of the new compounds and their absolute configurations were established on the basis of spectroscopic analysis including NMR, MS and ECD.

Three new highly oxygenated sterols and one new dihydroisocoumarin from the marine sponge-derived fungus Cladosporium sp. SCSIO41007.

Three new highly oxygenated sterols (1-3) and a new dihydroisocoumarin (7) together with six known compounds were isolated from the extracts of the culture of a sponge-derived fungus Cladosporium sp. SCSIO41007. The structures of all new compounds (1-3, 7) were determined by the extensive spectroscopic analysis including NMR, MS, IR, and UV.

Isobenzofuranones and Isochromenones from the Deep-Sea Derived Fungus Leptosphaeria sp. SCSIO 41005.

Four new isobenzofuranones, leptosphaerins J-M (-), including an unusual naturally-occurring centrosymmetric dimer skeleton (), and two new isochromenones, clearanols I-J (-), were obtained from a culture of a deep-sea sediment-derived fungus sp. SCSIO 41005, together with four known isobenzofuranones (-) and six known isochromenones (-). These structures were elucidated by extensive spectroscopic analyses, and absolute configurations were assigned on the basis of electronic circular dichroism and optical rotations data comparison.