Biomineralization of Human Genomic DNA into ZIF-8, a Zeolite-Like Metal-Organic Framework.

Unlabelled: was to assess the capabilities of human genomic DNA biomineralization into ZIF-8 metal-organic framework (MOF) preserving DNA sequence integrity after the encapsulation cycle and composite dissolving. The study is an initial stage of the project aimed at developing an abiotic vector to be used when working with native nucleic acids of an arbitrary size based on DNA@ZIF-8 composite.

Materials And Methods: We studied human genomic DNA isolated from lymphocytes of peripheral blood of healthy volunteers using Proba-NK kit (DNA-Technology LLC, Russia).

Non-viral systems for intracellular delivery of genome editing tools.

A hallmark of the last decades is an extensive development of genome editing systems and technologies propelling genetic engineering to the next level. Specific and efficient delivery of genome editing tools to target cells is one of the key elements of such technologies. Conventional vectors are not always suitable for this purpose due to a limited cargo volume, risks related to cancer and immune reactions, toxicity, a need for high-purity viral material and quality control, as well as a possibility of integration of the virus into the host genome leading to overexpression of the vector components and safety problems.

[Geriatric status of patients over 70 years of age with myocardial infarction and the relationship with the severity of myocardial stress, fibrosis and angiogenesis.].

A prospective study of 92 patients over 70 years old with myocardial infarction was carried out. All patients were diagnosed and determined the severity of geriatric syndromes, including fragility, using specialized scales on the 5th day of hospitalization. The comorbidity was assessed with the calculation of the Charlson Comorbidity Index, levels of NT-proBNP, ST-2, FGF-21, VEGF in blood serum by enzyme immunoassay.

[Experience with 13-valent conjugated pneumococcal vaccine in HIV-infected patients].

Aim: Evaluate the effect of PCV13 vaccination on the composition of the microflora of the upper respiratory tract and the immune system in HIV-infected patients.

Materials And Methods: 100 patients with HIV-infection were included in the study. The patients underwent immunological examination and the collection of biomaterial from the posterior pharyngeal wall for microbiological examination.

[The range and potential contribution of irreversibly adsorbed proteins on the surface of artificial implants].

Protein adsorption is the first stage of the interaction between prosthetic materials with tissues of the body. They undergo conformational changes depending on the chemical composition and the nanotopography surface. Adsorbed proteins induce adhesion and alter the functional state of migrating cells.

[Atrial Fibrillation After Coronary Artery Bypass Grafting].

Purpose: to identify the role of factors of inflammation and myocardial ischemia in the development of atrial fibrillation (AF) in the early postoperative period after coronary artery bypass grafting (CABG).

Methods: We enrolled in this study 96 patients with ischemic heart disease who had undergone CABG. Patients were divided into 2 groups: (1) without postoperative AF (n=67, 80.

[Assessment of the relationship of inflammation markers, adipokines and parameters of lipid metabolism in patient].

Objective - to assess the changes and the relationship of inflammation markers, adipokine levels and lipid metabolism in overweight and obesity, with signs of the metabolic syndrome. The study involved 62 women aged from 25 to 50 years. Group 1 included women (n=20) with a BMI<27.

[Prevention of inflammatory complications after osteoplastic operations in oral cavity].

It was established that every patient carried out through the oral osteoplastic operation needs immunoprophylaxis to avoid postoperative complications. It doesn't depend on the initial level of the immunity. Asa casual prophylaxis Licopid is recommented.

[Peripheral blood responses in elderly patients with some prevalent diseases].

The aim of the research was to study blood responses to inflammatory processes in patients of different ages. The subjects of the study were 31 healthy persons and 198 patients, 116 of whom had pneumonia and 82 had chronic pyelonephritis. The patients were divided into three age groups: 16 to 39 years, 40 to 59 years, and older than 60 years.