Publications by authors named "Liliia Ibneeva"
Article Synopsis
- A specific group of dormant hematopoietic stem cells (dHSCs) acts as a reserve pool to protect against exhaustion, but too much dormancy can hinder responses to blood system stress.
- Researchers discovered that CD38 serves as a key marker for identifying dHSCs and found that the cADPR signaling pathway, regulated by CD38, plays a crucial role in maintaining dHSC dormancy by influencing a protective cell cycle inhibitor.
- Understanding this CD38/cADPR/Ca2+/c-Fos/p57Kip2 pathway could lead to new methods to enhance stem cell transplant effectiveness and promote blood recovery post-injury or illness.
Objective: To develop a simple robust methodology of screening multiple CHO cell clones secreting recombinant proteins to assess their specific productivity.
Results: We developed a dual assay based on immunoassay measurements of a recombinant protein expression combined with staining of viable cells with resazurin. Following this approach, colonies can be simultaneously assessed for cell growth rate and for production of a recombinant protein.