Publications by authors named "Lilian Maria de Castilho"
Highly Defined Induced Pluripotent Stem Cell Lines Mimic Donor Red Blood Cell Antigen Profiles for Therapeutic and Diagnostic Use.
Cell Reprogram
June 2024
Article Synopsis
- - Our team developed two induced pluripotent stem cell (iPSC) lines from blood donors to produce red blood cells (RBCs) tailored for transfusions, particularly for patients with sickle cell disease, as well as for creating RBC panel reagents.
- - The iPSCs were created by reprogramming enriched erythroblast populations from the donors' blood using nonintegrative plasmid vectors, and both iPSC lines retained the unique erythrocyte antigen profiles of their respective donors.
- - These iPSC lines, which showed self-renewal and potential for hematopoietic differentiation, could lead to more efficient methods for producing donor-independent RBCs, thus meeting specific transfusion needs in the future
Frequency and characterization of RHD variant alleles in a population of blood donors from southeastern Brazil: Comparison with other populations.
Transfus Apher Sci
August 2021
Background: The correct determination of D antigen could help to avoid alloimmunization in pregnant women and patients receiving blood transfusions. However, there are limitations in the identification of D variants as the partial and weak D phenotypes make the determination of D antigen a great challenge in the transfusion routine.'
Study Design And Methods: The molecular characterization of D variants was performed on blood donors from southeastern Brazil with atypical D typing.
Background: We evaluated different technological approaches and anti-D clones to propose the most appropriate serologic strategy in detecting the largest numbers of D variants in blood donors.
Methods: We selected 101 samples from Brazilian blood donors with different expressions of D in our donor routine. The tests were performed in immediate spin (IS) with eleven commercially available anti-D reagents in a tube and microplate.
Background: Dombrock blood group system genotyping has revealed various rearrangements of the Dombrock gene and identified new variant alleles in Brazil (i.e., DO*A-SH, DO*A-WL and DO*B-WL).
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