Revealing the elevated sulfite levels in acute kidney injury using a promising ratiometric fluorescent probe.

The development of novel biomarkers for the early diagnosis of acute kidney injury (AKI) is critical for enabling timely protective interventions and predicting potential drug toxicity. Recent studies have highlighted metabolic abnormalities in sulfur-containing compounds during AKI progression. A ratiometric fluorescent probe, BP-BT-OH, has been designed for sulfite detection, featuring a covalent bond between benzothiazole and benzopyran-oxonium.

Targeting endothelial cells: A novel strategy for pulmonary fibrosis treatment.

Endothelial cells (ECs) are a monolayer of flat cells lining the inner surfaces of blood and lymphatic vessels. They play a key role in many physiological and pathological processes. Specifically, they maintain vascular permeability and structural stability and participate in immune responses, inflammation, coagulation, and other vital functions.

Ultra-high dose rate radiotherapy overcomes radioresistance in head and neck squamous cell carcinoma.

Radiotherapy (RT) resistance in head and neck squamous cell carcinoma (HNSCC) significantly hampers local control and patient prognosis. This study investigated the efficacy and molecular mechanisms of high-energy X-ray-based ultra-high dose rate radiotherapy (UHDR-RT) in overcoming RT resistance. The established RT-resistant HNSCC cell lines and animal models were subjected to UHDR-RT or conventional RT (Conv-RT) via a high-power rhodotron accelerator.

Ultrasensitive Determination of Amino Acids in Single Cells by Chemical Isotope Labeling with Liquid Chromatography Mass Spectrometry Analysis.

Amino acids play multiple critical roles in the regulation of various metabolic pathways and physiological processes in living organisms. Mass spectrometry (MS) has become the most pioneering platform for amino acid analysis. However, the simultaneous and sensitive determination of amino acids is still challenging because of their structural similarity and broad ranges of concentrations.

Multifaceted roles of ninjurin1 in immunity, cell death, and disease.

Ninjurin1 (NINJ1) is initially identified as a nerve injury-induced adhesion molecule that facilitates axon growth. It is initially characterized to promote nerve regeneration and mediate the transendothelial transport of monocytes/macrophages associated with neuroinflammation. Recent evidence indicates that NINJ1 mediates plasma membrane rupture (PMR) in lytic cell death.

Validation and development of a refined M1 category for nasopharyngeal carcinoma based on the version-nine of AJCC/UICC TNM staging system in the immunotherapy era: A multicenter cohort study.

Objective: To evaluate the applicability of the M1 category of the version-nine of AJCC/UICC TNM staging system (TNM-9) for M1 nasopharyngeal carcinoma (M1-NPC) in immunotherapy era and propose potential refinements.

Methods: M1-NPC patients who underwent palliative chemotherapy and immune checkpoint inhibitors (ICIs) between January 2019 and June 2023 across five institutions were included and re-staged according to TNM-9. Overall survival (OS) and Progression-free survival (PFS) were analyzed.

Application of graphene oxide in Agrobacterium-mediated genetic transformation and construction of a novel DNA delivery system for watermelon.

Graphene oxide (GO) is widely used in biotechnology. The purpose of this study was to improve the efficiency of genetic transformation by constructing a delivery system based on GO. First, GO was applied in the traditional genetic transformation scheme for watermelons.

Bioinformatics-based Prognosis Effect of Ferroptosis-related Genes on Gastric Cancer.

This study investigated the relationship between ferroptosis related genes and the prognosis of gastric cancer patients. Gene expression and corresponding clinical data were obtained from the TCGA database. Differentially expressed genes in gastric cancer were identified using R software, and prognostic-related genes were screened using univariate Cox regression analysis.

Molecular Subtyping and Therapeutic Targeting of IFNG-Driven Immunogenic Cell Death in Lung Adenocarcinoma.

Background: Immunogenic cell death (ICD) can be triggered by various therapies to induce anti-tumor immune responses, significantly enhancing treatment effectiveness, and is widely utilized in tumor immunotherapy.

Methods: LUAD data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) validated ICD-related molecular subtypes via consensus clustering. Clinical features, ICD genes, driver genes, mutations, tumor microenvironment, immune checkpoints, and drug sensitivity were compared.

Novel methods for genetic transformation of watermelon () without tissue culture via .

Unlabelled: Factors that restrict the development of genetic transformation include the long cycle, extensive requirements for experimental conditions, and low survival and transformation rates. Especially for plants that obtain offspring through sexual reproduction. This study established the genetic transformation methods that are particularly suitable for it.

N-Alkylation of 1,2,3-Triazoles with Ethers under Thermodynamical Control.

Herein, a relay strategy incorporating oxidative cross dehydrogenative coupling (CDC) and N- to N-isomerization is disclosed for the formal N-selective alkylation of triazoles with ethers under thermodynamic control conditions. By taking advantage of the different thermodynamical stabilities of N- and N-alkylated triazoles (3.65 kcal/mol), the initially formed N-isomers can be converted into their more stable N-isomers when reaching thermodynamic equilibrium.

A Local Adversarial Attack with a Maximum Aggregated Region Sparseness Strategy for 3D Objects.

The increasing reliance on deep neural network-based object detection models in various applications has raised significant security concerns due to their vulnerability to adversarial attacks. In physical 3D environments, existing adversarial attacks that target object detection (3D-AE) face significant challenges. These attacks often require large and dispersed modifications to objects, making them easily noticeable and reducing their effectiveness in real-world scenarios.

Tamsulosin ameliorates bone loss by inhibiting the release of Cl through wedging into an allosteric site of TMEM16A.

TMEM16A, a key calcium-activated chloride channel, is crucial for many physiological and pathological processes such as cancer, hypertension, and osteoporosis, etc. However, the regulatory mechanism of TMEM16A is poorly understood, limiting the discovery of effective modulators. Here, we unveil an allosteric gating mechanism by presenting a high-resolution cryo-EM structure of TMEM16A in complex with a channel inhibitor that we identified, Tamsulosin, which is resolved at 2.

Impact of menopausal status on cognitive function in female papillary thyroid carcinoma patients: a longitudinal propensity score matched study.

Purpose: Previous research has documented cognitive deficits in survivors of papillary thyroid carcinoma (PTC).Our longitudinal study with large sample size, aims to assess the impact of menopausal status on cognitive function, elucidate related factors of cognitive impairment, and chart the trajectory of cognitive changes over time in female PTC patients.

Methods: In this longitudinal study, we administered the Montreal Cognitive Assessment (MoCA) to 322 female PTC patients over 40 years old, before surgery and at 3 and 6 months after surgery.

Multimodal Contrastive Learning for Remote Sensing Image Feature Extraction Based on Relaxed Positive Samples.

Traditional multimodal contrastive learning brings text and its corresponding image closer together as a positive pair, where the text typically consists of fixed sentence structures or specific descriptive statements, and the image features are generally global features (with some fine-grained work using local features). Similar to unimodal self-supervised contrastive learning, this approach can be seen as enforcing a strict identity constraint in a multimodal context. However, due to the inherent complexity of remote sensing images, which cannot be easily described in a single sentence, and the fact that remote sensing images contain rich ancillary information beyond just object features, this strict identity constraint may be insufficient.

Trolox derivatives: Synthesis, structure-activity relationship and promote wound healing by regulating oxidative stress and inflammation.

To find new antioxidants, 13 Trolox amides (2a-2m) and 7 Trolox esters (3a-3g) were synthesized and evaluated for their anti-inflammatory and antioxidant activity. Compounds 2e, 2i, 3b and 3d showed potent anti-inflammatory and antioxidant activity, amongst them, 3d demonstrated the most photoprotective effects on UVB-irradiated human skin keratinocyte (HaCaT) cells (IC = 5.13 µM) through efficiently scavenging free radicals and dose-dependently reducing reactive oxygen species (ROS) and apoptosis generation, as well as effectively promoting wound healing.

Regulated cell death in acute myocardial infarction: Molecular mechanisms and therapeutic implications.

Acute myocardial infarction (AMI), primarily caused by coronary atherosclerosis, initiates a series of events that culminate in the obstruction of coronary arteries, resulting in severe myocardial ischemia and hypoxia. The subsequent myocardial ischemia/reperfusion (I/R) injury further aggravates cardiac damage, leading to a decline in heart function and the risk of life-threatening complications. The complex interplay of multiple regulated cell death (RCD) pathways plays a pivotal role in the pathogenesis of AMI.

Soft-lithographically defined template for arbitrarily patterned acoustic bioassembly.

Acoustic bioassembly is recently regarded as a highly efficient biofabrication tool to generate functional tissue mimics. Despite their capacity of directly patterning live cells with close intercellular proximity, most acoustic bioassembly techniques are currently limited to generate some specific simple types of periodic and symmetric patterns, which represents an urgent challenge to emulate geometrically complex cytoarchitecture in human tissue. To address this challenge, we herein demonstrate a soft-lithographically defined acoustic bioassembly (SLAB) technique that enables to assemble live cells into geometrically defined arbitrary multicellular structures.

Characteristics of blood lipid and metabolic indicators in subclinical hypothyroidism patients: a retrospective study.

Background: The characteristics of blood lipid and metabolic indicators were analyzed in patients with subclinical hypothyroidism (SCH), and the effect of type 2 diabetes mellitus (T2DM) on SCH patients was determined.

Methods: The physical examination data of 2,119 residents in a university community were retrospectively divided into 2 groups (SCH and non-SCH groups). Furthermore, the SCH group was divided into SCH-T2DM and SCH-non-T2DM subgroups, and the data between the groups were analyzed.

A bibliometric and visual analysis of Parkinson's disease sleep disorders: articles from 2008 to 2023.

Objective: Sleep disorder is a common non-motor symptom (NMS) of Parkinson's disease. However, the global research focus on Parkinson's sleep-related disorders (PDSDs) and future trends remains unclear. Currently, there is no bibliometric analysis of PDSDs.

Targeting the immune privilege of tumor-initiating cells to enhance cancer immunotherapy.

Tumor-initiating cells (TICs) possess the ability to evade anti-tumor immunity, potentially explaining many failures of cancer immunotherapy. Here, we identify CD49f as a prominent marker for discerning TICs in hepatocellular carcinoma (HCC), outperforming other commonly used TIC markers. CD49f-high TICs specifically recruit tumor-promoting neutrophils via the CXCL2-CXCR2 axis and create an immunosuppressive milieu in the tumor microenvironment (TME).