Epsins 1 and 2 promote NEMO linear ubiquitination via LUBAC to drive breast cancer development.

Estrogen receptor-negative (ER-negative) breast cancer is thought to be more malignant and devastating than ER-positive breast cancer. ER-negative breast cancer exhibits elevated NF-κB activity, but how this abnormally high NF-κB activity is maintained is poorly understood. The importance of linear ubiquitination, which is generated by the linear ubiquitin chain assembly complex (LUBAC), is increasingly appreciated in NF-κB signaling, which regulates cell activation and death.

Incidence and risk factors associated with postoperative stroke in the elderly patients undergoing hip fracture surgery.

Objectives: Stroke is one of the rare but devastating complications after hip fracture in the elderly. By far, there is still scarce data on postoperative stroke in elderly patients with hip fractures.

Methods: This was a retrospective study of prospectively collected data.

Association between maternal smoking during pregnancy and offspring overweight in U.S.-born children.

Background: The criticism of the literature on smoking during pregnancy and offspring overweight was the confounding from postnatal factors. Interaction between gestational age and prenatal maternal smoking has not yet assessed.

Methods: We used the Third National Health and Nutrition Examination Survey (1988-1994), linked with natality files of US-born singletons aged 2 to 6 years.

Clinical factors affecting prognosis of limb osteosarcoma in China: a multicenter retrospective analysis.

Objective: This study was performed to explore the relationship between various clinical factors and the prognosis of limb osteosarcoma.

Methods: We retrospectively analyzed the clinical data of 336 patients with limb osteosarcoma treated from June 2000 to August 2016 at 7 Chinese cancer centers. Data on the patients' clinical condition, treatment method, complications, recurrences, metastasis, and prognosis were collected and analyzed.

Laparoscopic versus open hepatectomy for elderly patients with hepatocellular carcinoma.

Purpose: The number of surgical operations for elderly patients with hepatocellular carcinoma increases as the population ages. The aim of this study was to evaluate surgical and survival outcomes in elderly patients who underwent laparoscopic or open hepatectomy for hepatocellular carcinoma.

Methods: We analyzed the data of 169 patients aged 70 or over who underwent hepatectomy for hepatocellular carcinom between January 2013 and December 2018.

Initial data release and announcement of the 10,000 Fish Genomes Project (Fish10K).

Background: With more than 30,000 species, fish-including bony, jawless, and cartilaginous fish-are the largest vertebrate group, and include some of the earliest vertebrates. Despite their critical roles in many ecosystems and human society, fish genomics lags behind work on birds and mammals. This severely limits our understanding of evolution and hinders progress on the conservation and sustainable utilization of fish.

Molecular Dynamics Simulations of Mite Aquaporin DerfAQP1 from the Dust Mite (Acariformes: Pyroglyphidae).

Aquaporins are a large family of transmembrane channel proteins that facilitate the passive but highly selective transport of water and other small solutes across biological membranes. House dust mite () is the major source of household immunogens, and we have recently reported six cDNA sequence encoding aquaporins from this mite species. To better understand the structure and role of mite aquaporin, we constructed a tertiary structure for DerfAQP1 by homology modeling from the X-ray structure of malaria aquaporin PfAQP (Protein Data Bank code No.

Epsin-mediated degradation of IP3R1 fuels atherosclerosis.

The epsin family of endocytic adapter proteins are widely expressed, and interact with both proteins and lipids to regulate a variety of cell functions. However, the role of epsins in atherosclerosis is poorly understood. Here, we show that deletion of endothelial epsin proteins reduces inflammation and attenuates atherosclerosis using both cell culture and mouse models of this disease.

Identification of continuous immunoglobulin G epitopes of Dermatophagoides farinae allergens by peptide microarray immunoassay.

Dermatophagoides farinae, as a common house dust mite species, is one of the main sources of allergens in the world. At present, Dermatophagoides farinae is found to contain more than 30 groups of allergens. These allergens are used for allergen-specific immunotherapy (AIT) of allergic diseases.

[Effect of lentivirus-mediated shRNA targeting down-regulation of PPARα on perfluorododecanoic acid induced oxidative damage in rat hepatocytes BRL 3A].

Objective: To investigate the role of peroxisome proliferator-activated receptor alpha(PPARα) in perfluorododecanoic acid(PFDoA)-induced liver oxidative damage in rats by observing lentivirus-mediated shRNA targeting and down-regulating PPARα expression in rat hepatocytes BRL 3 A.

Methods: A PPARα lentivirus-compatible shRNA interference vector Lenti-iPα and a negative control vector Lenti-NC were constructed, and co-transfected with lentivirus packaging helper plasmids into 293 FT cells for lentivirus packaging. The lentivirus stock solution was collected, concentrated and the virus titer was determined.

The Combination of IgA and IgG autoantibodies against Transcriptional Intermediary Factor-1γ contributes to the early diagnosis of Lung Cancer.

The aberrant expression of tumor-associated antigens (TAAs) is responsible for the release of large amounts of autoantibodies in sera, and serum autoantibody detection has been demonstrated to contribute to the early diagnosis of malignancies. Recent studies showed the closely correlation of transcriptional intermediary factor-1γ (TIF1γ) with some malignancies. In our pilot study, we found aberrantly high expression of TIF1γ protein existed in cancer tissues other than matched paracancerous tissues of patients with lung cancer (LC) at early stage by immunohistochemistry (IHC) staining.

Penehyclidine hydrochloride inhibits renal ischemia/reperfusion-induced acute lung injury by activating the Nrf2 pathway.

The nuclear factor (NF)-κB and NOD-like receptor protein 3 (NLRP3) pathways promote inflammatory signaling that injures the kidneys, whereas the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway promotes anti-inflammatory signaling that inhibits oxidative damage. Penehyclidine hydrochloride (PHC) inhibits NF-κB and activates Nrf2 signaling. We investigated whether PHC induces communication between the Nrf2 and NF-κB/NLRP3 pathways, thereby protecting against renal ischemia/reperfusion (rI/R)-induced lung inflammation.

Confucian values, trust, and family farm adoption of green control techniques.

Encouraging farmers to adopt green control techniques (GCTs) helps to reduce and control the use of chemical pesticides. However, there is a lack of attention regarding the promotion of culture as an informal institution. As an important part of Chinese culture, Confucian values affect the behavior and decision-making of Chinese people imperceptibly, and these values are more visceral for farmers.

LncRNA MEG3 suppressed the progression of ovarian cancer via sponging miR-30e-3p and regulating LAMA4 expression.

Background: Ovarian cancer (OC) is a common female reproductive malignancy with a high mortality rate. Although LAMA4 was observed to be downregulated in OC cells, its mechanism in regulating OC metastasis is still unknown. This study aimed to investigate the effect of LAMA4 and its mechanism on OC.

A novel mutation in TRIOBP gene leading to congenital deafness in a Chinese family.

Background: The autosomal recessive non-syndromic deafness DFNB28 is characterized by prelingual sensorineural hearing loss. The disease is related with mutations in TRIOBP (Trio- and F-actin-Binding Protein) gene, which has three transcripts referred to as TRIOBP-5, TRIOBP - 4 and TRIOBP-1. Among them, TRIOBP-5/- 4 are expressed in the inner ears and crucial for maintaining the structure and function of the stereocilia.

Correlation of microRNA-146a/b with disease risk, biochemical indices, inflammatory cytokines, overall disease severity, and prognosis of sepsis.

Background: Previous studies have indicated the association of microRNA-146a/b (miR-146a/miR-146b) with pro-inflammatory cytokines production, lipopolysaccharide-mediated injuries and organ dysfunction, however, the correlation of miR-146a/miR-146b with disease risk, disease severity, biochemical indices, inflammatory cytokines and mortality of sepsis has not been explored, which was investigated in the present study.

Methods: In total, 180 sepsis patients and 180 healthy controls were enrolled. The peripheral blood samples were collected from sepsis patients within 24 hour after admission and from healthy controls at enrolment.

Graphene ultraviolet ultrahigh-Q perfect absorption for nanoscale optical sensing.

We propose an ultraviolet perfect ultranarrow band absorber by coating a dielectric grating on the monolayer graphene-dielectric-metal structure. The absorber presents an ultranarrow Fano lineshape with quality (Q) factor of 70 and a nearly perfect absorption of over 99.9% in the ultraviolet region, which is ascribed to the near field coupling of the optical dissipation of graphene and guide mode resonance of the dielectric grating.

Cellular UAP56 interacts with the HBx protein of the hepatitis B virus and is involved in viral RNA nuclear export in hepatocytes.

UAP56 is an essential factor in eukaryotic pre-mRNA splicing and mRNA export. Many viruses require cellular RNA export factors to efficiently export viral RNA. However, the mechanisms behind hepatitis B virus (HBV) RNA splicing and nuclear export remain poorly understood.

Regression multiple imputation for missing data analysis.

Iterative multiple imputation is a popular technique for missing data analysis. It updates the parameter estimators iteratively using multiple imputation method. This technique is convenient and flexible.

Association between CYP2B6 polymorphism and acute leukemia in a Han population of Northwest China.

Background: The aim of this study was to investigate potential associations between CYP2B6 c.516G>T polymorphism and the occurrence and prognosis of acute leukemias (AL) in the Han population of Northwest China.

Methods: The CYP2B6 gene polymorphism was analyzed by PCR-RFLP and Sanger DNA sequencing in 126 patients with AL and 161 healthy controls.

The protective effect of doxofylline against lipopolysaccharides (LPS)-induced activation of NLRP3 inflammasome is mediated by SIRT1 in human pulmonary bronchial epithelial cells.

Lung diseases are common health problems in many countries. The dysfunction of bronchial epithelial cells is important for the development of lung diseases. Recent progress reveals that inflammasome is the fundamental mechanism of epithelial activation.

Load swing rejection for double-pendulum tower cranes using energy-shaping-based control with actuator output limitation.

For the controller design of tower cranes, the swing of load is always regarded the crane as a single-pendulum model, however in practical applications, such as when the hook mass can not be ignored, the tower crane dynamic system presents a double-pendulum nature. In this case, the dynamic characteristics analysis and controller design for such cranes becomes more complicated. In addition, many researches do not take into account the real situation of the actuator, that is, in actual tower cranes, the output torque/force can not be infinite due to the actual design constraints of the actuator.

MTR4 drives liver tumorigenesis by promoting cancer metabolic switch through alternative splicing.

The metabolic switch from oxidative phosphorylation to glycolysis is required for tumorigenesis in order to provide cancer cells with energy and substrates of biosynthesis. Therefore, it is important to elucidate mechanisms controlling the cancer metabolic switch. MTR4 is a RNA helicase associated with a nuclear exosome that plays key roles in RNA processing and surveillance.