Expression of bovine interleukin 15 in and study on its biological activity: a T-cell activator.

Interleukin 15 (IL-15) plays crucial role as stimulatory cytokine in generation and proliferation of CD8 T memory cells. The present study was initiated to analyze the role of yeast-expressed bovine IL-15 in inducing the CD8 T memory cells. The bIL-15 was cloned in pPICZαA expression vector.

Probing the Protein-Protein Interaction Network of Proteins Causing Maturity Onset Diabetes of the Young.

Protein-protein interactions (PPIs) play vital roles in various cellular pathways. Most of the proteins perform their responsibilities by interacting with an enormous number of proteins. Understanding these interactions of the proteins and their interacting partners has shed light toward the field of drug discovery.

Parasites and pathological conditions in the edible oyster, Crassostrea madrasensis (Preston), from the east and west coasts of India.

A detailed pathological survey was carried out on the commercially important edible oyster, Crassostrea madrasensis (Preston), from two distinct coastal/brackish water ecosystems of south India. Samples were collected twice a year during wet and dry seasons from 2009 to 2012. Bacterial colonies in the form of prokaryotic inclusions, protozoans (Perkinsus beihaiensis, Nematopsis sp.

Expression of biologically active bovine interleukin 7 and evaluating the activity in vitro.

Aim: Interleukin 7 (IL-7) is a ϒc family cytokine involved in the homeostatic proliferation and maintenance of immune cells. In the present study, we report the expression of bovine IL-7 (bIL-7) in Escherichia coli and evaluated for its biological activity.

Materials And Methods: The sequence coding for bIL-7 (mature protein) was amplified from primary bovine kidney cell culture and cloned into pET28-a vector and expressed in E.

Expression of bovine interleukin 15 and evaluation of its biological activity in vitro.

Background/aim: Recent studies have shown that interleukin-15 (IL-15)is a critical factor for the development and proliferation of CD8(+) memory T cells. The aim of present study is to study the role bovine IL-15 (bIL-15)in activation pathway of bovine CD8(+) T cells if any, which will be useful in designing the adjuvant to increase the duration of immunity of the vaccine preparations.

Materials And Methods: Coding region of bIL-15 (489) was amplified from cDNA of lipopolysaccharide-induced bovine peripheral blood mononuclear cells (PBMCs) using gene specific primers and cloned into pcDNA3.

First report of Perkinsus beihaiensis in Crassostrea madrasensis from the Indian subcontinent.

Protozoan parasites of the genus Perkinsus are considered important pathogens responsible for mass mortalities in many wild and farmed bivalve populations. The present study was initiated to screen populations of the Indian edible oyster Crassostrea madrasensis, a promising candidate for aquaculture along the Indian coasts, for the presence of Perkinsus spp. The study reports the presence of P.