One-Pot Synthesis of Hydroxylated Alkaloids from Sugars via a Pictet-Spengler-Type Reaction.

An efficient and convenient strategy has been successfully developed for the preparation of novel hydroxylated alkaloid derivatives (also called fused multicyclic iminosugars) from -toluenesulfonylated sugars through a Pictet-Spengler-type mechanism. This method is highly stereoselective, does not require metal catalysts, and capable of conducting gram level reactions (with a 53% yield). Some of such iminosugars had an intermediate antiproliferative effect on HCT116 tumor cells.

Variation of microbial necromass carbon and its potential relationship with humification during composting of chicken manure with and without biochar addition.

Microbial necromass carbon (MNC) is an important stable organic C component. However, the variation of MNC and its potential relationship with humus components in composting remains uncertain. During a 45-day chicken manure composting study with and without biochar, MNC ranged from 24.

Comparison and Analysis of Computational Methods for Identifying N6-Methyladenosine Sites in Saccharomyces cerevisiae.

Background: N-methyladenosine (mA) plays critical roles in a broad range of biological processes. Knowledge about the precise location of mA site in the transcriptome is vital for deciphering its biological functions. Although experimental techniques have made substantial contributions to identify mA, they are still labor intensive and time consuming.

Recent Advances on Antioxidant Identification Based on Machine Learning Methods.

Antioxidants are molecules that can prevent damages to cells caused by free radicals. Recent studies also demonstrated that antioxidants play roles in preventing diseases. However, the number of known molecules with antioxidant activity is very small.

Sequence based prediction of pattern recognition receptors by using feature selection technique.

Pattern recognition receptors (PRRs) play crucial roles in the innate immune system, and are able to identify pathogen-associated molecular patterns and damage-associated molecular patterns. Accurate identification of PRRs is essential for understanding their functions. In the present work, a random forest based method was proposed to identify PRRs, in which the sequences were formulated by using the optimal features.

Modified cellulose by polyethyleneimine and ethylenediamine with induced Cu(II) and Pb(II) adsorption potentialities.

In order to synthesize adsorbent that can effectively absorb heavy metals in a simple and economical way, this study described the fabrication of adsorbents based on cellulose acetate (CA). CA modified first by polyethyleneimine grafting (CAP) and then by ethylenediamine (CAPE) for enhancing Cu(II) and Pb(II) removal from water. The physico-chemical properties of the modified celluloses were analyzed.

R132H mutation in IDH1 gene reduces proliferation, cell survival and invasion of human glioma by downregulating Wnt/β-catenin signaling.

Mutations in the isocitrate dehydrogenase 1 (IDH1) gene commonly occur in gliomas. Remarkably, the R132H mutation in IDH1 (IDH1-R132H) is associated with better prognosis and increased survival than patients lacking this mutation. The molecular mechanism underlying this phenomenon is largely unknown.

Diagnostic Value of Different Systematic Prostate Biopsy Methods in the Detection of Prostate Cancer with Ultrasonographic Hypoechoic Lesions--A Comparative Study.

Objective: To assess if a less extended biopsy in the transperineal approach is sufficient for detection of prostate cancer (PC) in patients with hypoechoic lesions.

Methods: This was a prospective study of 167 consecutive patients with prostate hypoechoic lesion and who underwent transperineal ultrasound (TPUS)-guided 12-core and hypoechoic lesion core biopsy between January 2012 and February 2013.

Results: PC was detected in 64.

A new 5-grading score in the diagnosis of prostate cancer with real-time elastography.

Objective: To evaluate the clinical significance of transrectal real-time elastography (TRTE) in diagnosis of prostate cancer (PCA).

Methods: 195 patients with an elevated PSA level were enrolled in the study. A novel 5-grading score of prostate outer gland was applied by TRTE imaging.