Sodium Deoxycholate-Propidium Monoazide Droplet Digital PCR for Rapid and Quantitative Detection of Viable HN001 in Compound Probiotic Products.

As a famous probiotic, HN001 is widely added to probiotic products. Different strains have different probiotic effects, and the active HN001 strain is the key to exerting probiotic effects, so it is of great practical significance for realising the detection of HN001 at the strain level in probiotic products. In this study, strain-specific primer pairs and probes were designed.

Metagenomic profiles of the antimicrobial resistance in traditional Chinese fermented meat products: Core resistome and co-occurrence patterns.

Antimicrobial resistance (AMR) poses a significant challenge to global health, and the presence of antibiotic resistance genes (ARGs) in food poses a potential threat to public health. Traditional Chinese fermented meat products (FMPs) are highly favored because of their unique flavors and cultural value. However, microbial safety and the potential distribution and composition of AMR in these products remain unclear.

Metagenomics reveals differences in the composition of bacterial antimicrobial resistance and antibiotic resistance genes in pasteurized yogurt and probiotic bacteria yogurt from China.

Antimicrobial resistance has become a global public health concern, and antibiotic resistance genes (ARG) in food are a research focus. In China, probiotics and pasteurized yogurts are the 2 main types of commercially available yogurt, but the distribution and differences of antibiotic-resistant bacteria and gene types in these products are not well known. This study used a shotgun metagenomic approach to analyze 22 different types of yogurt collected from 9 main yogurt-producing areas in China; each type of yogurt included 8 different batches of samples.

Single-molecule real-time sequencing reveals differences in bacterial diversity in raw milk in different regions and seasons in China.

The quality of raw milk is a key factor influencing the whole dairy processing chain. The richness and diversity of bacteria in raw milk affect its quality and safety. However, traditional microbial detection methods mainly depend on the known microbe culture and are often time consuming.

Application of metabolomics analysis to aid in understanding the pathogenicity of different lineages and different serotypes of Listeria monocytogenes.

Listeria monocytogenes is a foodborne pathogen with high mortality in young children, elderly persons, pregnant women, and immune-compromised individuals. Most human listeriosis cases are associated with four serotypes (1/2a, 1/2b, 1/2c and 4b) within lineages I and II. The intracellular metabolic changes in L.

Insight into Associated with Infant Foods in Beijing.

This study was undertaken to investigate the prevalence, antimicrobial resistance, and virulence gene profiles of in different brands of infant formula in Beijing supermarkets. Eighty-eight isolates were recovered in sixty-eight infant formulas of five domestic brands and fourteen imported brands. The prevalence rate in domestic and imported samples were 70.

Enhanced response of sensor on serotonin using nickel-reduced graphene oxide by atomic layer deposition.

Atomic layer deposition (ALD) is a promising method for preparing nanomaterials. The thickness and uniformity of nanomaterials can be precisely controlled. Hence, the uniform Ni nanoparticles (Ni NPs) deposited on reduced graphene oxide (rGO) by ALD and got the optimal combination interface.

Response of Formed-Biofilm of Enterobacter cloacae, Klebsiella oxytoca, and Citrobacter freundii to Chlorite-Based Disinfectants.

Unlabelled: Bacterial biofilms formed on equipment surfaces are potential sources of cross-contamination and can be responsible for the spread of bacteria involved in food spoilage, such as some Enterobacteriaceae family members. In this study, the effect of chlorite-based disinfectants, including sodium hypochlorite (SH), chlorine dioxide (CD), strongly acidic electrolyzed water (StAEW), and neutral electrolyzed water (NEW), on inactivation of mono-biofilms of Enterobacter cloacae, Klebsiella oxytoca, and Citrobacter freundii was evaluated separately. All the strains were enumerated by the viable plate-count method after disinfection for 30 min.

Preliminary Transcriptome Analysis of Mature Biofilm and Planktonic Cells of Enteritidis Exposure to Acid Stress.

has emerged as a well-recognized food-borne pathogen, with many strains able to form biofilms and thus cause cross-contamination in food processing environments where acid-based disinfectants are widely encountered. In the present study, RNA sequencing was employed to establish complete transcriptome profiles of Enteritidis in the forms of planktonic and biofilm-associated cells cultured in Tryptic Soytone Broth (TSB) and acidic TSB (aTSB). The gene expression patterns of .

Visual and light scattering spectrometric method for the detection of melamine using uracil 5'-triphosphate sodium modified gold nanoparticles.

A highly selective method was presented for colorimetric determination of melamine using uracil 5'-triphosphate sodium modified gold nanoparticles (UTP-Au NPs) in this paper. Specific hydrogen-bonding interaction between uracil base (U) and melamine resulted in the aggregation of AuNPs, displaying variations of localized surface plasmon resonance (LSPR) features such as color change from red to blue and enhanced localized surface plasmon resonance light scattering (LSPR-LS) signals. Accordingly, the concentration of melamine could be quantified based on naked eye or a spectrometric method.

Ratiometric fluorescence recognition for pyrophosphate on the basis of terpyridine derivative.

Diphosphate (pyrophosphate, PPi) is vital for organisms, and therefore its detection is of special importance. In this paper, one cadmium complex of terpyridine (tpy) derivative, 4'-(aminomethylphenyl)-2,2':6',2"-terpyridine (aptpy), has been reported for the ratiometric fluorescence recognition of PPi. When added with cadmium, the emission of aptpy at 358 nm was greatly enhanced and red shifted to 397 nm due to the complexation-induced ICT process, which then blue shifted to 349 nm upon the further addition of PPi.

[Study on panchromatic band broadening of new high-resolution satellite sensor].

For developing a remote sensor, the selection of operating waveband is one of the most important factors for detecting and identifying target. In the present paper, the changes of atmospheric effects and imagery quality are simulated due to the increase in the response wave range of optical remote sensor from 0.50-0.