Publications by authors named "Liisa Kuhi"
Objectives: Detection of antinuclear antibodies (ANA) by indirect immunofluorescence assay using HEp-2 cells (HEp-2 IFA) is used to screen for various autoimmune diseases. HEp-2 IFA suffers from variability, which hampers harmonization.
Methods: A questionnaire was developed to collect information on HEp-2 IFA methodology, computer-assisted diagnosis (CAD) systems, training, inter-observer variability, quality assessment, reagent lot change control, and method verification.
Objectives: No reference data are available on repositories to measure precision of autoantibody assays. The scope of this study was to document inter- and intra-run variations of quantitative autoantibody assays based on a real-world large international data set.
Methods: Members of the European Autoimmunity Standardisation Initiative (EASI) group collected the data of intra- and inter-run variability obtained with assays quantifying 15 different autoantibodies in voluntary participating laboratories from their country.
Introduction: The first wave of COVID-19 pandemic has disrupted almost all areas of the health care services to some extent throughout the world. Although the negative impact of COVID-19 on patients with autoimmune diseases has also been recognized, available data in this regard are limited. In the current study of the European Autoimmunity Standardisation Initiative (EASI) we aimed to provide reliable data on the extent of the impact of COVID-19 pandemic on test requests for different autoantibodies in European countries.
View Article and Find Full Text PDFOne of the unmet needs to be addressed is prognostic biomarkers for early knee osteoarthritis (kOA). We aimed to study the association of urinary collagen type-II C-terminal cleavage neoepitope (uC2C) with the emergence and progression of kOA. The longitudinal data of 330 subjects (aged 32-60 years) from an Estonian population-based cohort were used.
View Article and Find Full Text PDFArticle Synopsis
- The International Consensus on Antinuclear Antibody Patterns (ICAP) aims to standardize the reporting of ANA IIF patterns, differentiating between competent and expert levels.
- A survey conducted with input from 438 lab professionals and 248 clinicians across 67 countries revealed that most nuclear patterns are widely reported, while cytoplasmic patterns show more variability in recognition.
- The study highlighted that nuclear patterns like centromere and homogeneous are seen as clinically significant, while patterns like DFS and certain cytoplasmic types lack consensus on their relevance.
Cartilage collagen neoepitope C2C in urine as an integrative diagnostic marker for early knee osteoarthritis.
Osteoarthr Cartil Open
December 2020
Objective: To investigate the suitability of urinary collagen type-II C-terminal cleavage neoepitope (uC2C) as a marker for early knee osteoarthritis (kOA).
Design: We examined 302 Estonian subjects (mean age, 49 years): 186 subjects with and 20 control subjects without knee symptoms, and 96 patients treated by arthroscopy. For the latter, cartilage lesions were characterized using Société Francaise d'Arthroscopie (SFA) scores.