Lipid nanoparticles as adjuvant of norovirus VLP vaccine augment cellular and humoral immune responses in a TLR9- and type I IFN-dependent pathway.

Norovirus (NoV) virus-like particles (VLPs) adjuvanted with aluminum hydroxide (Alum) are common vaccine candidates in clinical studies. Alum adjuvants usually inefficiently assist recombinant proteins to induce cellular immune responses. Thus, novel adjuvants are required to develop NoV vaccines that could induce both efficient humoral and robust cellular immune responses.

ETV6-NTRK2 Fusion in a Patient With Metastatic Pulmonary Atypical Carcinoid Successfully Treated With Entrectinib: A Case Report and Review of the Literature.

Pulmonary atypical carcinoid (AC) is an extremely rare neuroendocrine tumor. The neurotrophic tropomyosin receptor kinase (NTRK) fusions are reported in only 0.5% of nonsmall cell lung cancer, and are more rare in AC with only one previously reported case.

Using Variational Quantum Algorithm to Solve the LWE Problem.

The variational quantum algorithm (VQA) is a hybrid classical-quantum algorithm. It can actually run in an intermediate-scale quantum device where the number of available qubits is too limited to perform quantum error correction, so it is one of the most promising quantum algorithms in the noisy intermediate-scale quantum era. In this paper, two ideas for solving the learning with errors problem (LWE) using VQA are proposed.

Longitudinal Immune Profiling Highlights CD4+ T Cell Exhaustion Correlated with Liver Fibrosis in Schistosoma japonicum Infection.

Schistosomiasis remains an important public health concern. The eggs deposited in livers invoke a Th2-dominant response, which mediates the fibrotic granulomatous response. However, the mechanisms involved in this immunopathological process are still not perfectly clear.

Atmospheric aerosol particle size distribution from Lidar data based on the lognormal distribution mode.

Analysis of the atmospheric Aerosol Particle Size Distribution (APSD) retrieved from Light Detection and Ranging (Lidar) data is one of the popular fields in atmospheric remote sensing. An APSD retrieval method based on the lognormal particle size distribution, the Mie theory, and the Second Simulation of the Satellite Signal in the Solar Spectrum (6S) were studied. According to the 6S, this method divides the main body of the aerosols into four basic components and calculates the APSD from Lidar data and the optical and microphysical characteristics of these components.

6-Valent Virus-Like Particle-Based Vaccine Induced Potent and Sustained Immunity Against Noroviruses in Mice.

Norovirus is a major cause of acute gastroenteritis worldwide, and no vaccine is currently available. The genetic and antigenic diversity of Norovirus presents challenges for providing broad immune protection, which calls for a multivalent vaccine application. In this study, we investigated the possibility of developing a virus-like particle (VLP)-based 6-valent Norovirus vaccine candidate (Hexa-VLPs) that covers GI.

Genetic profiles of non-syndromic severe-profound hearing loss in Chinese Hans by whole-exome sequencing.

Hereditary hearing loss is highly heterogeneous. Despite over 120 non-syndromic deafness genes have been identified, there are still some of novel genes and variants being explored. In the study, we investigated 105 Chinese Han children with non-syndromic, prelingual, severe-profound hearing loss by whole-exome sequencing on DNA samples.

Distribution and transport characteristics of fine particulate matter in beijing with mobile lidar measurements from 2015 to 2018.

Accurately quantifying the concentration and transport flux of atmospheric fine particulate matter (PM) is vital when attempting to thoroughly identify the pollution formation mechanism. In this study, the mobile lidar measurements in Beijing on heavily polluted days in December from 2015 to 2018 are presented. The lidar was mounted on a vehicle, which could perform measurements along designated routes.

Vertical profile of aerosols in the Himalayas revealed by lidar: New insights into their seasonal/diurnal patterns, sources, and transport.

Atmospheric aerosols play a crucial role in climate change, especially in the Himalayas and Tibetan Plateau. Here, we present the seasonal and diurnal characteristics of aerosol vertical profiles measured using a Mie lidar, along with surface black carbon (BC) measurements, at Mt. Qomolangma (QOMS), in the central Himalayas, in 2018-2019.

Multi-Center in-Depth Screening of Neonatal Deafness Genes: Zhejiang, China.

Purpose: The conventional genetic screening for deafness involves 9-20 variants from four genes. This study expands screening to analyze the mutation types and frequency of hereditary deafness genes in Zhejiang, China, and explore the significance of in-depth deafness genetic screening in newborns.

Methods: This was a multi-centre study conducted in 5,120 newborns from 12 major hospitals in the East-West (including mountains and islands) of Zhejiang Province.

Investigation of atmospheric ozone during summer and autumn in Guangdong Province with a lidar network.

The overall ozone concentration in China has increased significantly in recent years, as has the atmospheric oxidization, and both of these changes are impacting human activities. Measurements based on differential absorption lidar (DIAL) were conducted from June to October 2019 in order to investigate the vertical distribution of ozone in Guangdong Province. The vertical and horizontal distributions of ozone in six different cities were systematically examined during the summer and autumn and analyzed the two different ozone characteristics (local pollution and regional transport).

Comprehensive study of regional haze in the North China Plain with synergistic measurement from multiple mobile vehicle-based lidars and a lidar network.

Recently, haze pollution has emerged as a regional characteristic that needs to be monitored and mitigated sensibly in China, particularly in the North China Plain (NCP). Clarifying the distribution and source characteristics of haze is necessary to better understand its formation mechanism on a regional scale. In this study, a comprehensive study of regional haze using synergistic measurement from multiple mobile vehicle-based lidars, a ground-based lidar network, and in suit instruments is presented.

Succession of fungal dynamics and their influence on physicochemical parameters during pig manure composting employing with pine leaf biochar.

The effects of pine leaf biochar (PLB) on fungal community during pig manure composting were investigated. Five different doses of PLB [0% (T1), 2.5% (T2), 5% (T3), 10% (T4) and 15% (T5)] were mixed with mixture of pig manure and sawdust (2:1) for 50 days of composting.

A Perfused Microfluidic System to Study the Differentiation of Neural Stem Cells in vitro.

Introduction: Due to the ability to mimic in vivo cellular microenvironments, the development of multicell culture systems has received increasing interest for use as research models and serving as platforms for drug evaluation.

Methods: In this study, we developed a perfused microfluidic system to resemble the in vivo intercellular environment and applied it to study the differentiation from neural stem cells into neurons.

Results: As determined by immunofluorescence chemistry and quantitative real-time PCR, the neural stem cells grown in this microfluidic system showed an elevated differentiation rate toward the formation of neurons as determined by the increased level of βIII-tubulin production, which is 4 times higher than that of culturing neural stem cells only.

Intrabullous Adhesion Pexia (IBAP) by Percutaneous Pulmonary Bulla Centesis: An Alternative for the Surgical Treatment of Giant Pulmonary Bulla (GPB).

Background And Objective: Most patients with giant pulmonary bulla (GPB) are treated by surgery; however, there is a subset for whom surgery is not a viable option, such as those with contraindications, or those unwilling to undergo operation. Therefore, an alternative minimally invasive method is desired for this subpopulation. The aim of this study was to explore an alternative procedure for treating GPB.

[Seizure outcome after surgery for medically intractable mesial temporal lobe epilepsy and its predictors].

Objective: To evaluate seizure outcome in patients receiving surgery for chronic medically intractable mesial temporal lobe epilepsy (MTLE) and analyze its possible predictors.

Methods: This retrospective study was conducted in patients with chronic medically intractable MTLE undergoing anterior temporal lobectomy (ATL) or selective amygdalohippocampectomy (SAH) in our department between September, 2011 and October, 2013. The patients were followed up for 3.

Secreted protein acidic and rich in cysteine enhances the chemosensitivity of pancreatic cancer cells to gemcitabine.

It has been previously shown that the simultaneous exposure of colon cancer cells MIP to irinotecan and secreted protein acidic and rich in cysteine (SPARC) enhances anticancer activity. However, whether there is same effect of SPARC in pancreatic cancer remains largely unknown. Therefore in this study, we aimed to investigate the role of SPARC played in the sensitivity of pancreatic cancer to gemcitabine.

Soluble expression of active recombinant human tissue plasminogen activator derivative (K2S) in Escherichia coli.

Context: The kringle 2 plus serine protease domains (K2S) of human tissue plasminogen activator (tPA) is an efficacious thrombolytic drug, which has been used to treat heart attacks and strokes by breaking up the clots that cause them. It has nine disulfide bridges, which are needed for proper folding and be the bottleneck in improving the production in the Escherichia coli system. So far, few reports have described the production of soluble active K2S from E.

High-level expression of the antimicrobial peptide plectasin in Escherichia coli.

Plectasin is a defensin-like antimicrobial peptide isolated from a fungus, the saprophytic ascomycete Pseudoplectania nigrella. Plectasin showed marked antibacterial activity in vitro against Gram-positive bacteria, especially Streptococcus pneumoniae, including strains resistant to conventional antibiotics. Plectasin could kill the sensitive strain as efficaciously as vancomycin and penicillin and without cytotoxic effects on mammalian cell viability.