[Feasibility Analysis of Establishing Combat Readiness Blood Bank Based on Low Titer Group O Whole Blood and Group A Plasma].

Objective: To explore the feasibility of establishing combat readiness blood bank with low titer group O whole blood and group A plasma.

Methods: The Galileo automatic blood analyzer was used to detect the titers of IgM anti-A and anti-B antibodies in the samples of group O blood donors and IgM anti-B titer in the samples of group A blood donors. Group O blood donors with antibody titers below 128 were selected and included in the mobile blood bank for combat readiness, group A plasma with anti-B titer lower than 128 and group O whole blood with antibody titers below 128 were included in the combat readiness entity blood bank.

Application of anti-D immunoglobulin in D-negative pregnant women in China.

This article summarizes the current situation of anti-D immunoglobulin (anti-D-Ig) use in RhD-negative pregnant women at home and abroad. The article describes the concept, research and development history, and domestic and foreign applications of anti-D-Ig and points out that anti-D-Ig has not been widely used in China, mainly due to reasons such as unavailability in the domestic market and non-standard current application strategies. The article focuses on analyzing the genetic and immunological characteristics of RhD-negative populations in China.

The application of generalized S-transform in the denoising of surface plasmon resonance (SPR) spectrum.

In order to obtain accurate resonance peaks from surface plasmon resonance (SPR) spectral curves, a reasonable denoising method is of great significance for SPR sensing systems. Therefore, the generalized S-transform is combined with the Bald Eagle Search algorithm (BES) in this study, and a denoising method based on the generalized S-transform optimized by BES is proposed and applied to the denoising processing of the SPR spectrum. First, a fiber SPR sensing system is used to obtain the original noised spectrum; then, the generalized S-transform is performed to obtain the corresponding S-domain spectrum.

Application of magnetic resonance neuroimaging in determining the relationship between tumors and peripheral nerves.

Background: Magnetic resonance imaging (MRI) is commonly used to analyze the relationship between tumors and nerves before surgery. However, the application value of diffusion tensor imaging (DTI), diffusion weighted imaging (DWI), and post-processing techniques needs further elucidation.

Purpose: To assess the value of DTI, DWI, and various post-processing techniques in determining the relationship between tumors and nerves.

[Platelet Transfusion Strategies for MASPAT-Matched Platelet Transfusion Failed Patient with Allogeneic Hematopoietic Stem Cell Transplantation].

Objective: To investigate the causes of ineffectiveness of platelet transfusion with monoclonal antibody solid phase platelet antibody test (MASPAT) matching in patients with allogeneic hematopoietic stem cell transplantation and explore the strategies of platelet transfusion.

Methods: A case of donor-specific HLA antibodies (DSA) induced by transfusion which ultimately resulted in transplantation failure and ineffective platelet transfusion with MASPAT matching was selected, and the causes of ineffective platelet transfusion and platelet transfusion strategy were retrospectively analyzed.

Results: The 32-year-old female patient was diagnosed as acute myeloid leukemia (high risk) in another hospital with the main symptoms of fever and leukopenia, who should be admitted for hematopoietic stem cell transplantation after remission by chemotherapy.

[Correlation Analysis of Hemolytic Transfusion Reaction Induced by Low Titer Antibody].

Objective: To establish the diagnostic process of low titer blood group antibody in the occurrence of adverse reactions of hemolytic transfusion.

Methods: Acid elusion test, enzyme method and PEG method were used for antibody identification. Combined with the patient's clinical symptoms and relevant inspection indexes, the irregular antibodies leading to hemolysis were detected.

Influence of Polysaccharides From on Short-Chain Fatty Acid Production and Quorum Sensing in .

Polysaccharide is one of the main active ingredients of , which has been proven to regulate the balance of gut microbiota. For the first time, this study focused on the regulation of polysaccharides from (PS) on , a specific probiotic in the intestinal tract. PS effectively promoted the biomass, biofilm and acetic acid production in 2-84, and enhanced quorum sensing (QS) signaling.

A single-center investigational study of CD36 antigen deficiency and platelet alloantibody distribution in different populations in Northern China as well as platelet alloantibodies effect on pregnancy.

Background: Platelet antibodies can lead to clinical diseases such as platelet transfusion refractoriness (PTR), fetal/neonatal alloimmune thrombocytopenia (FNAIT), etc. This study is aimed at understanding CD36 expression, platelet alloantibody distribution in different populations in Northern China, and effects of platelet alloantibodies on pregnancy.

Study Design And Methods: Whole blood samples of 612 subjects including hematological patients, pregnant women, and blood donors were collected at a single center, then CD36 expressions were determined, followed by platelet antibody screening and characterization of platelet antibody specificity.

[Effect of 25 Gy (60)Co Irradiation on the Physico-chemical Property and Functions of the Platelets During Storage].

Objective: To evaluate the effects of the 25 Gy ⁶⁰Co irradiation on the physiological and biochemical properties and the functions of the platelets during storage.

Methods: A total of 15 bags of platelets were apheresis-collected from 15 healthy donors, and each bag of platelets were divided into 2 parts, then the platelets were divided into the control group (without 25 Gy ⁶⁰Co irradiation) and the irradiated group (with 25 Gy ⁶⁰Co irradiation) groups. The two groups of platelets were kept under the condition of (22 ± 2) °C and shaken.

Label-free fluorescence method for screening G-quadruplex ligands.

G-quadruplex ligands can interfere with the telomere structure, telomere elongation/replication, and proliferation of cancer cells. A key element in the development of potent G-quadruplex ligands is the screening of large chemical libraries of potential candidates. Here, we describe a simple fluorescence method for screening of G-quadruplex ligands.

One-pot fluorescence detection of multiple analytes in homogenous solution based on noncovalent assembly of single-walled carbon nanotubes and aptamers.

We have developed a new multicolor fluorescent sensing system to detect multiple analytes in one pot. This design is based on the noncovalent assembly of dye-labeled aptamer with single-walled carbon nanotubes (SWNTs) by π-stacking between the nucleotide bases and the SWNTs sidewalls. In the presence of the targets, the aptamer-target binding separates the assembly of dye-labeled aptamers and SWNTs, resulting in the restoration of fluorescence signal of the dye labeled with aptamers.

[Establishment of genotyping method for fetal ABO group from pregnant maternal peripheral blood].

This study was aimed to establish a genotyping method to detect ABO group gene of fetus from peripheral blood of pregnant women for prenatal diagnosis of hemolytic disease of newborn (HDN) resulting from ABO blood group incompatibility. 4 pairs of primers were designed according to ABO blood group gene DNA and mRNA sequences. 20 plasma DNA samples from healthy donors were extracted and amplified to explore the best conditions for plasma DNA extraction and PCR amplification.