[Effects of on Proliferation, Migration and Invasion of Acute Myeloid Leukemia Cells by Regulating /HOXA9 Axis].

Objective: To explore the effects and molecular mechanism of on the proliferation, migration and invasion of acute myeloid leukemia (AML) cells.

Methods: Bone marrow samples from 35 pediatric AML patients and 35 healthy controls in Henan Provincial Children's Hospital from April 2015 to April 2017 and human bone marrow stromal cell lines (HS-5) and AML cell lines (HL-60, THP-1, U-937 and Kasumi-1) were collected. The expressions of and homeobox A9 (HOXA9) in bone marrow samples and cells were detected by RT-qPCR and Western blot.

[Expression and Prognostic Impact of in Bone Marrow of Patients with Newly Diagnosed Acute Myeloid Leukemia].

Objective: To detect the expression level of gene in the bone marrow of newly diagnosed patients with acute myeloid leukemia (AML) and investigate its influence on the clinical characteristics and prognosis.

Methods: The expression level of gene in the bone marrow of 90 newly diagnosed patients with AML that accompanying clinical characteristics and survival status were detected by RT-qPCR, and compared with 18 allogeneic hematopoietic stem cell transplantation (allo-HSCT) donors. The Chi-square test, Kaplan-Meier survival analysis, and Cox proportional hazards regression model were used to analyze the correlation of expression level with clinical characteristics and prognosis.

[Effect of breastfeeding on immune function in infants with human cytomegalovirus infection].

Objectives: To study the effect of breastfeeding on immune function in infants with human cytomegalovirus (HCMV) infection.

Methods: A retrospective analysis was performed on the medical data of 135 infants with HCMV infection who were admitted to Children's Hospital Affiliated to Zhengzhou University from January 2021 to May 2022, and all these infants received breastfeeding. According to the results of breast milk HCMV-DNA testing, the infants were divided into two groups: breast milk HCMV positive (=78) and breast milk HCMV negative (=57).

[Clinicopathological Analysis of Children's Systemic EBV-Positive T-Cell Lymphoma].

Objective: To explore the clinical and pathologic features as well as prognosis of systemic EBV-positive T-cell lymphoma in children.

Methods: The clinical data including clinical manifestation, pathologic changes and treatment in 16 patients with children's systemic EBV-positive T-cell lymphoma were analyzed retrospectively, and follow-up of patients were carried out.

Results: The 16 cases included 12 males and 4  females with median age of 3.

Withaferin A activates stress signalling proteins in high risk acute lymphoblastic leukemia.

Withaferin A, the principal bio-active component isolated from the Withaniasomnifera, has shown promising anti-leukemic activity in addition to anti-invasive and anti-metastatic activity. The present study demonstrates the effect of withaferin A on the cell cycle status and the phosphorylation/activation of proteins involved in signal transduction in t(4;11) and non-t(4;11) acute lymphoblastic leukemia (ALL) cell lines after treatment with withaferin A. The cells after treatment with the vehicle or 25 μM withaferin A for 1, 2, 4 and 8 h were examined using flow cytometric analysis.

Conditioned medium from hypoxia-treated adipocytes renders muscle cells insulin resistant.

Adipose tissue hypoxia is an early phenotype in obesity, associated with macrophage infiltration and local inflammation. Here we test the hypothesis that adipocytes in culture respond to a hypoxic environment with the release of pro-inflammatory factors that stimulate macrophage migration and cause muscle insulin resistance. 3T3-L1 adipocytes cultured in a 1% O2 atmosphere responded with a classic hypoxia response by elevating protein expression of HIF-1α.

Synthesis and biological activity of novel tiliroside derivants.

A series of new tiliroside derivatives were synthesized and characterized by analytical (1)H NMR, (13)C NMR and mass spectrometry. All of the compounds were evaluated for anti-diabetic properties in vitro using HepG2 cells. Compounds 3c, 3d, and 3i-l caused significant enhancements in glucose consumption by insulin-resistant HepG2 cells compared with control cells and cells that were exposed to metformin (an anti-diabetic drug).

Tiliroside-derivatives enhance GLUT4 translocation via AMPK in muscle cells.

Tiliroside isolated from Chinese herb Potentilla chinensis showed therapeutic activities in diabetes. We synthesized 7 tiliroside-derivatives and examined their effects on surface GLUT4myc levels in muscle cells. Derivatives 2a and 3 increased surface GLUT4myc levels, and derivative 3 has the greatest potential.