Relationship Between the Triglyceride-Glucose Index and Type 2 Diabetic Macroangiopathy: A Single-Center Retrospective Analysis.

Purpose: The research explores the relationship between the triglyceride-glucose index (TyG index) and the macroangiopathy risk in single-center hospitalized type 2 diabetes mellitus (T2DM) patients and develops a risk prediction nomogram model.

Patients And Methods: A total of 858 patients with T2DM were studied retrospectively. Lasso regression was used to eliminate unimportant factors, and multivariate logistic regression analysis was used to investigate the association between the TyG index and macrovascular disease in T2DM.

σ-NagA-L1/L2 Regulatory Circuit Involved in -Mediated Increase in β-Lactam Susceptibility in Stenotrophomonas maltophilia.

OmpA, the most abundant porin in Stenotrophomonas maltophilia KJ, exists as a two-domain structure with an N-terminal domain of β-barrel structure embedded in the outer membrane and a C-terminal domain collocated in the periplasm. KJΔOmpA, an mutant of S. maltophilia KJ with a truncated OmpA devoid of 299 to 356 amino acids (aa), was able to stably embed in the outer membrane.

Effect of treatment zone decentration on axial length growth after orthokeratology.

Objective: To study the effect of treatment zone (TZ) decentration on axial length growth (ALG) in adolescents after wearing the orthokeratology lenses (OK lenses).

Materials And Methods: This retrospective clinical study selected 251 adolescents who were fitted OK lenses at the Clinical College of Ophthalmology, Tianjin Medical University (Tianjin, China) from January 2018-December 2018 and wore them continuously for >12 months. The age of the subjects was 8-15 years, spherical equivalent (SE): -1.

Sp1-Induced SETDB1 Overexpression Transcriptionally Inhibits HPGD in a β-Catenin-Dependent Manner and Promotes the Proliferation and Metastasis of Gastric Cancer.

Purpose: Gastric cancer (GC) has high morbidity and mortality, the cure rate of surgical treatment and drug chemotherapy is not ideal. Therefore, development of new treatment strategies is necessary. We aimed to identify the mechanism underlying Sp1 regulation of GC progression.

Vitamin D Receptor Activation Targets ROS-Mediated Crosstalk Between Autophagy and Apoptosis in Hepatocytes in Cholestasic Mice.

Background & Aims: Observational epidemiologic studies have associated vitamin D deficiency with cholestasis. We reported previously that activation of the vitamin D/vitamin D receptor (VDR) axis in cholangiocytes mitigates cholestatic liver injury by remodeling the damaged bile duct. However, the function of VDR in hepatocytes during cholestasis remains unclear.

Rapid and ultrasensitive detection of SARS-CoV-2 spike protein based on upconversion luminescence biosensor for COVID-19 point-of-care diagnostics.

Here, we firstly introduce a detection system consisting of upconversion nanoparticles (UCNPs) and Au nanorods (AuNRs) for an ultrasensitive, rapid, quantitative and on-site detection of SARS-CoV-2 spike (S) protein based on Förster resonance energy transfer (FRET) effect. Briefly, the UCNPs capture the S protein of lysed SARS-CoV-2 in the swabs and subsequently they are bound with the anti-S antibodies modified AuNRs, resulting in significant nonradiative transitions from UCNPs (donors) to AuNRs (acceptors) at 480 nm and 800 nm, respectively. Notably, the specific recognition and quantitation of S protein can be realized in minutes at 800 nm because of the low autofluorescence and high Yb-Tm energy transfer in upconversion process.

Cannabidiol attenuates methamphetamine-induced cardiac inflammatory response through the PKA/CREB pathway in rats.

Background: Methamphetamine (MA) abuse is a major global public health problem. However, it is not yet known whether cannabidiol (CBD) has protective effects on MA-induced cardiotoxicity. The present study investigated whether CBD has protective effects on MA-induced cardiac damage in rats via the protein kinase A/cyclic adenosine monophosphate (cAMP)-response element-binding protein (PKA/CREB) pathway.

Multiplexed detection of SARS-CoV-2 based on upconversion luminescence nanoprobe/MXene biosensing platform for COVID-19 point-of-care diagnostics.

Multiplexed detection is essential in biomedical sciences since it is more efficient and accurate than single-analyte detection. For an accurate early diagnosis of COVID-19, a multiplexed detection strategy is required to avoid false negatives with the existing gold standard assay. NbCT nanosheets were found to efficiently quench the fluorescence emission of lanthanide-doped upconversion luminescence nanoparticles at wavelengths ranging from visible to near-infrared spectrum.

Incorporation of NGR1 promotes bone regeneration of injectable HA/nHAp hydrogels by anti-inflammation regulation a MAPK/ERK signaling pathway.

Suitable bone grafts are commonly required to achieve successful bone regeneration, wherein much effort has been spent to optimize their osteogenesis. Increasing evidence has demonstrated that reducing the levels of TNF-α can enhance bone regeneration at the injury site. Notoginsenoside R1 (NGR1) has been extensively studied in the field of anti-inflammation and regenerative medicine.

Loss of OTUD6B Stimulates Angiogenesis and Promotes Diabetic Atherosclerosis.

Purpose: Angiogenesis is an essential promoter of atherosclerotic plaque rupture. However, the mechanism of its regulation is not understood. OTUD6B regulates cell proliferation, migration, and angiogenesis.

Effect of ultramarathon running on iron metabolism.

Background: Iron is a vital trace element for energy production and oxygen transportation; importantly, it is essential to athletic performance. Maintaining iron balance is tightly controlled at systemic and cellular levels. This study aimed to determine serum iron tests, hepcidin levels, and cellular iron import and export activities in peripheral blood mononuclear cells (PBMCs) in ultramarathon runners to elucidate the association of systemic inflammation response and iron metabolism.

Tris(bipyridine)ruthenium(II)-functionalized metal-organic frameworks for the ratiometric fluorescence determination of aluminum ions.

A novel ratiometric fluorescence probe was designed for the determination of Al by self-assembling of NH-MIL-101(Fe) and [Ru(bpy)]. Under the excitation wavelength of 360 nm, the NH-MIL-101(Fe)@[Ru(bpy)] presented a dual-emitting luminescent property at 440 and 605 nm, respectively. In the presence of Al, the blue fluorescence of NH-MIL-101(Fe)@[Ru(bpy)] at 440 nm was enhanced remarkably, while the red emission at 605 nm was almost not influenced.

Effects of nanoplastic exposure on the immunity and metabolism of red crayfish (Cherax quadricarinatus) based on high-throughput sequencing.

Previous studies have shown that nanoplastics (NPs) are harmful pollutants that threaten aquatic organisms and ecosystems, however, less research has been conducted on the hazards of NPs for aquaculture animals. In this study, Cherax quadricarinatus was used as an experimental model to evaluate the possible effects of three concentrations (25, 250 and 2500 μg/L) of NPs on red crayfish. The toxicological effects of NPs on this species were investigated based on transcriptomics and microbiome.

Methamphetamine and HIV-1 Tat proteins synergistically induce microglial autophagy via activation of the Nrf2/NQO1/HO-1 signal pathway.

Methamphetamine (METH) is a psychostimulant that is abused throughout the world. METH is a highly addictive drug commonly used by persons living with HIV, and its use can result in cognitive impairment and memory deficits. METH and human immunodeficiency virus-1 transactivator of transcription (HIV-1Tat) have toxic and synergistic effects on the nervous system; however, the mechanism of their synergistic effects has not been clarified.

An integrated metabolome and transcriptome analysis of the L. petals reveal the molecular mechanisms of anthocyanin accumulation.

L. var. Shigyoku is a new double-flowered bluish-purple variety in China that changes color during flower development from bluish-purple to light purple.

Germanene-modified chitosan hydrogel for treating bacterial wound infection: An ingenious hydrogel-assisted photothermal therapy strategy.

The elaborate design of an ingenious hydrogel-assisted photothermal therapy (PTT) platform is a promising strategy for treating bacterial wound infections. Herein, a new generation of germanene nanocrystals (Ge NCs) with excellent photothermal performance are prepared via an ice-bath sonication liquid-phase exfoliation technique. Whereafter, by crosslinking interaction between chitosan and zinc acetate, as well as self-assembly property between Ge NCs and chitosan, we successfully construct an innovative germanene-modified chitosan antimicrobial hydrogel (CS/Ge NCs) integrating capture and killing bacteria performances.

Circadian signatures of adipose tissue in diet-induced obesity.

High-fat diet (HFD) feeding rewires circadian rhythms of peripheral organs including the liver and adipose tissue. While the liver has been extensively studied, it remains largely unknown whether and how HFD organizes circadian biology in adipose tissue. Here, we took a systems approach to profile the diurnal transcriptome of adipose tissue in diet-induced obese mice either fed a low-fat diet (LFD) that reduces weight or still fed HFD.

Knockdown of hepatocyte Perilipin-3 mitigates hepatic steatosis and steatohepatitis caused by hepatocyte CGI-58 deletion in mice.

Comparative gene identification-58 (CGI-58), also known as α/β hydrolase domain containing 5, is the co-activator of adipose triglyceride lipase that hydrolyzes triglycerides stored in the cytosolic lipid droplets. Mutations in CGI-58 gene cause Chanarin-Dorfman syndrome (CDS), an autosomal recessive neutral lipid storage disease with ichthyosis. The liver pathology of CDS manifests as steatosis and steatohepatitis, which currently has no effective treatments.

The predictive factors of successful sperm retrieval for men with Y chromosome AZFc microdeletion.

Purpose: To identify key predictors for successful sperm retrieval in men with AZFc microdeletion.

Methods: Totally, 71 infertile men with confirmed AZFc microdeletion were studied. For each patient, the endocrine profile including serum follicle stimulating hormone (FSH), luteinizing hormone, total testosterone, prolactin, and estradiol was recorded, along with intratesticular testosterone levels (ITT), age, and testicular size.

Identification of the novel natural product inhibitors of SHP2 from the plant Toona sinensis: In vitro and in silico study.

In this study, we tested the inhibitory activity of 45 natural products extracted from the plant Toona sinensis on SHP2 protein, and identified four natural product inhibitors. The natural product 1,2,3,6-Tetragalloylglucose (A-1) was first reported as a competitive inhibitor of SHP2, with an IC value of 0.20 ± 0.

[Potentilla anserina polysaccharide alleviates the damage of mouse glomerular mesangial cells induced by high glucose via promoting the expression of circ-AKT3].

Objective To explore the effect of Potentilla anserina polysaccharide on the injury of glomerular mesangial cells induced by high glucose and its possible mechanism. Methods High glucose was used to induce SV40 MES 13 mouse glomerular mesangial cells to establish a cell injury model, and 0.1, 0.

Semantic-Powered Explainable Model-Free Few-Shot Learning Scheme of Diagnosing COVID-19 on Chest X-Ray.

Chest X-ray (CXR) is commonly performed as an initial investigation in COVID-19, whose fast and accurate diagnosis is critical. Recently, deep learning has a great potential in detecting people who are suspected to be infected with COVID-19. However, deep learning resulting with black-box models, which often breaks down when forced to make predictions about data for which limited supervised information is available and lack inter-pretability, still is a major barrier for clinical integration.

A hybrid hemodynamic knowledge-powered and feature reconstruction-guided scheme for breast cancer segmentation based on DCE-MRI.

Automatically and accurately annotating tumor in dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI), which provides a noninvasive in vivo method to evaluate tumor vasculature architectures based on contrast accumulation and washout, is a crucial step in computer-aided breast cancer diagnosis and treatment. However, it remains challenging due to the varying sizes, shapes, appearances and densities of tumors caused by the high heterogeneity of breast cancer, and the high dimensionality and ill-posed artifacts of DCE-MRI. In this paper, we propose a hybrid hemodynamic knowledge-powered and feature reconstruction-guided scheme that integrates pharmacokinetics prior and feature refinement to generate sufficiently adequate features in DCE-MRI for breast cancer segmentation.