Soil nutrients and enzyme activities based on millet continuous cropping obstacles.

In order to evaluate the effects of continuous cropping of millet on soil nutrients and soil enzyme activities, the present study was based on four treatments of 2 years of continuous cropping (T1), 3 years of continuous cropping (T2), 4 years of continuous cropping (T3) and rotational cropping (CK), based on 4 years of no fertilizer positioning experiments, and the soil nutrients, soil enzyme activities and millets yields were determined, respectively. The results showed that with the increase of continuous cropping years, the millet yield decreased and was significantly lower than that of rotating with legume crops, and compared with CK, the yields of T1, T2 and T3 treatments were reduced by 8.92%, 13.

[Spatial Prediction Method of Farmland Soil Organic Matter in Weibei Dryland of Shaanxi Province].

Accurately predicting the spatial distribution of soil organic matter (SOM) content is of great significance for improving soil quality and improving the level of regional soil management. In order to explore the optimal model for predicting the SOM content of farmland in the Weibei Dryland of Shaanxi Province, the influence factors closely related to SOM content were selected as the modeling covariables, and a geographic detector, the ordinary kriging method (OK), geographic weighted regression model (GWR), partial least squares regression model (PLS), geographically weighted regression extended model (GWR), and random forest model (RF) were used to predict the spatial distribution of SOM content in training samples. Additionally, the validation set samples were used to compare and analyze the prediction accuracy of the five methods.

A meta-analysis of reamed versus unreamed intramedullary nailing for the treatment of closed tibial fractures.

Controversy exists regarding the clinical outcomes of reamed vs unreamed intramedullary nailing in the treatment of closed tibial fractures. This study assessed the effects of reamed vs unreamed intramedullary nailing for closed tibial fractures. The authors searched PubMed, EMBASE, BIOSIS, and the Cochrane Controlled Trials Register for randomized and quasi-randomized controlled clinical trials from January 1980 to June 2012 comparing reamed with unreamed intramedullary nailing for closed tibial fracture in adults.

Substance P activates the Wnt signal transduction pathway and enhances the differentiation of mouse preosteoblastic MC3T3-E1 cells.

Recent experiments have explored the impact of Wnt/β-catenin signaling and Substance P (SP) on the regulation of osteogenesis. However, the molecular regulatory mechanisms of SP on the formation of osteoblasts is still unknown. In this study, we investigated the impact of SP on the differentiation of MC3T3-E1 cells.

Neuropeptide SP activates the WNT signal transduction pathway and enhances the proliferation of bone marrow stromal stem cells.

Substance P (SP) mediates multiple activities in various cell types, such as proliferation, anti-apoptotic response, and inflammation. We have investigated the effects of SP, NK1 antagonist and DKK1 on proliferation of bone marrow stromal stem cells (BMSCs), as well as the underlying mechanism. Isolated BMSCs were exposed to SP (10(-8)  M) (group A), SP + NK1 antagonist (1 µM) (group B), SP + DKK1 (0.

The biological effects and mechanisms of calcitonin gene-related peptide on human endothelial cell.

Background: Calcitonin gene-related peptide (CGRP) is a neuropeptide distributed in bone tissue involved in bone remodeling. Previously we demonstrated that CGRP can promote proliferation and migration of endothelial cells, relating to the expression of vascular endothelial growth factor (VEGF) and focal adhesion kinase (FAK).

Methods: CGRP1 receptor expression in human umbilical vein endothelial cells (HUVECs) was examined by immunofluorescence microscopy and real-time PCR.

[Calcitonin gene-related peptide promoting migration of rat bone marrow mesenchymal stem cells and stimulating expression of vascular endothelial growth factor].

Objective: To explore the effects of calcitonin gene-related peptide (CGRP) on the migration of bone marrow mesenchymal stem cells (BMSCs) and vascular endothelial growth factor (VEGF) expression in vitro.

Methods: The BMSCs were isolated from Sprague Dawley rats using whole bone marrow adherence method. At 1, 2, and 3 weeks after culture, the expressions of CGRP receptor (CGRPR) was detected by Western blot.