Publications by authors named "Liheng Luo"
Article Synopsis
- Base editors allow for precise changes in genetic material without causing double-strand breaks, making them useful for disease modeling and gene therapy, but their use is limited due to the need for specific PAM sequences.
- Researchers developed four near-PAMless base editor versions that broaden the range of target sequences and found that both the PAM and surrounding sequences influence editing efficiency.
- The team created BEguider, a deep learning model that predicts editing results for these new editors and provided experimental editing data on 20,541 ClinVar sites, showing that previously difficult-to-edit variants can now be addressed accurately; the predictive tool and data are available for public use.
Article Synopsis
- Scientists used a special tool called SpRY to find important parts of DNA that control gene activity, which previous methods missed.* -
- They studied a specific area in the genome (10q22.1) related to red blood cells and discovered new elements affecting a gene called HK1.* -
- This new method helps researchers better understand how DNA works and can be used for studying many other genetic elements too.*
Correction for 'MiR-4458-loaded gelatin nanospheres target COL11A1 for DDR2/SRC signaling pathway inactivation to suppress the progression of estrogen receptor-positive breast cancer' by Jie Liu , , 2022, , 4596-4611, https://doi.org/10.1039/D2BM00543C.
View Article and Find Full Text PDFRNA interference is a promising way to treat cancer and the construction of a stable drug delivery system is critically important for its application. Gelatin nanospheres (GNs) comprise a biodegradable drug vehicle with excellent biocompatibility, but there are limited studies on its delivery and role in the stabilization of miRNA and siRNA. Breast cancer is the most diagnosed type of female cancer worldwide.
View Article and Find Full Text PDFWith recent advances in high-throughput next-generation sequencing, it is possible to describe the regulation and expression of genes at multiple levels. An assay for transposase-accessible chromatin using sequencing (ATAC-seq), which uses Tn5 transposase to sequence protein-free binding regions of the genome, can be combined with chromatin immunoprecipitation coupled with deep sequencing (ChIP-seq) and ribonucleic acid sequencing (RNA-seq) to provide a detailed description of gene expression. Here, we reviewed the literature on ATAC-seq and described the characteristics of ATAC-seq publications.
View Article and Find Full Text PDFArticle Synopsis
- Muscle atrophy, often resulting from mechanical unloading, leads to decreased muscle function and injury risk, with no effective therapies currently available.
- Research identified miR-194 as a key player in muscle differentiation and the inhibition of harmful proteins, making it a potential therapy for muscle atrophy, although its stability as a drug has been a concern.
- A novel gelatin nanosphere delivery system was developed to effectively transport miR-194, showing significant improvements in muscle fiber size and function in a model of muscle atrophy, highlighting its potential as a targeted treatment platform for related diseases.
Database Study on the Expression and Purification of Membrane Proteins.
Protein Pept Lett
December 2021
Membrane proteins are crucial for biological processes, and many of them are important to drug targets. Understanding the three-dimensional structures of membrane proteins are essential to evaluate their bio-function and drug design. High-purity membrane proteins are important for structural determination.
View Article and Find Full Text PDFNon-coding RNAs (ncRNAs) include miRNA, lncRNA, and circRNA. NcRNAs are involved in multiple biological processes, including chromatin remodeling, signal transduction, post-transcriptional modification, cell autophagy, carbohydrate metabolism, and cell cycle regulation. Triple negative breast cancer (TNBC) is notorious for high invasiveness and metastasis, poor prognosis, and high mortality, and it is the most malignant breast cancer, while the effective targets for TNBC treatment are still lacking.
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