A nitrogen-phosphorus dual-doped porous spore carbon (NP-PSC) positive electrode matrix was prepared using native auricularia auricula as solid medium based on the principle of biomass rot. Yeast was introduce and cultured by the auricularia auricula solid medium. The freeze-drying and carbonization activation processes made the materials present a three-dimensional porous spore carbon aerogel properties.
View Article and Find Full Text PDFA nitrogen-sulfur dual-doped black fungus porous carbon (NS-FPC) matrix was prepared with natural black fungus as the carbon source and cysteine as the nitrogen-sulfur source. A black fungus-based solution was obtained by hydrothermal treatment. After further carbonization activation and combination with sulfur processing, the NS-FPC/S positive electrode materials were prepared.
View Article and Find Full Text PDFFront Bioeng Biotechnol
September 2022
A simple hydrothermal process employing sucrose and glutathione as the source of carbon and nitrogen-sulfur, respectively, a porous carbon/sulfur composite material doped with nitrogen and sulfur (NSPCS) was synthesized. The detailed structure information of the material was characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and Raman spectroscopy. The morphology information was investigated through Scanning Electron Microscope (SEM) methods.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
January 2023
Mitochondria are the sites of respiration in cells, and they participate in many indispensable biological processes. Because variations in mitochondrial viscosity can lead to dysfunctions of mitochondrial structure and function (and even induce malignant diseases), new sensors that can accurately monitor changes in mitochondrial viscosity are essential. To better investigate these changes, we report the development and evaluation of a novel benzothiophene-quinoline-based fluorescent chemosensor (BQL) that was designed especially for monitoring mitochondrial viscosity.
View Article and Find Full Text PDFMitochondria, the main source of energy of cells, play a significant role in aerobic respiration process. Some stimulants can result in changes of mitochondrial microenvironments such as viscosity, pH and polarity. Abnormal changes of mitochondrial viscosity have been shown to relate to pathological activities and diseases.
View Article and Find Full Text PDFHydrogen peroxide (HO), one of the most important reactive oxygen species (ROS), can be generated endogenously in the liver and has been deemed as a biomarker for evaluating drug-induced liver injury (DILI). Therefore, it is highly crucial to construct an effective method for detecting HO in the liver in order to evaluate DILI. Herein, a neoteric dual-signal colorimetric fluorescent probe XH-2 for sensing hydrogen peroxide was engineered and synthesized.
View Article and Find Full Text PDFNucleolus imaging is important for the understanding of gene expression, proliferation, and growth of cells. Traditional nucleoli localization mainly relies on the use of RNA fluorescent probes which are required in large amounts. These probes also have low selectivity, thus causing the generated images to have high background noise and the localization of nucleoli to become vague.
View Article and Find Full Text PDFAnal Bioanal Chem
October 2020
Cysteine (Cys) takes part in redox balance in cells as an antioxidant, and imbalance of Cys content in the body can cause a variety of diseases. It is very important to develop a new fluorescent chemosensor to specifically detect Cys intracellular. In this work, a novel NIR fluorescent probe was constructed based on 3-ethyl-1,1,2-trimethyl-1H-benzo[e]indol-3-ium iodide and 5-(4-hydroxyphenyl)furan-2-carbaldehyde.
View Article and Find Full Text PDFA hydrothermal method has been employed to synthesize a green and one-pot carbon dots-based sensor for ratiometric monitoring and imaging lysosomal pH in living cells. The carbon dots were directly functionalized by abundant amino groups during synthesis and exhibited dual emission bands at 439 and 550 nm under single-wavelength excitation of 380 nm without any additional modification. In addition to its small size, the established sensor had good biocompatibility.
View Article and Find Full Text PDFHydrogen peroxide (HO), an important member of the family of reactive oxygen species (ROS), has a significant impact on cell signal transduction, energy conversion and immune responses of living organisms. Therefore, accurate detection of the content of HO in living cells is of vital importance. In this paper, we report on the synthesis of a novel colorimetric and near-infrared fluorescent probe HAA, a heterocyclic aromatic amine with acetyl group for the specific detection of both exogenous and endogenous HO in living cells.
View Article and Find Full Text PDFHighly sensitive specific and ultrasensitive fluorescent probes are employed for tracking ClO⁻ for revealing its various cellular functions in living cells. In this work, a near-infrared fluorescence probe, XS-1, has been developed for tracking ClO⁻. The experimental results demonstrated that the probe, XS-1, could determine ClO⁻ at the linear range of 0-2 μM with a low detection limit of 72 nM (λ = 580/626 nm).
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
February 2020
We designed and synthesized a water-soluble near-infrared (NIR) fluorescent probe with the recognition unit of the cyanine-like structure and acrylate group. Through an aromatic ring nucleophilic substitution reaction based on sulfhydryl moiety, an off-on fluorescence response toward cysteine (Cys) was realized. The probe exhibited excellent spectral performance with an emission wavelength of 720nm and a detection limit of 0.
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