[Prokaryotic expression and helicase activity analysis of PDCoV NSP13].

Porcine deltacoronavirus (PDCoV) is a major pathogen causing fatal diarrhea in suckling piglets, and there is currently a lack of effective vaccines and drugs to prevent and control the virus. The nonstructural protein 13 (NSP13) serves as a virus-coded helicase and is considered to be a crucial target for antiviral drugs, making it imperative to investigate the helicase activity of NSP13. In this study, the gene of PDCoV was synthesized and integrated into the prokaryotic expression vector pET-28a to construct the recombinant plasmid pET-28a-NSP13.

Complete Genome Sequencing of Mycoplasma synoviae Strain HN01, Isolated from Chicken in Henan Province, China.

Here, we report the complete genome sequence of HN01, a virulent epidemic strain isolated from a sick chicken with synovitis in Henan Province, China. HN01 is the Asian source of an strain that is completely sequenced, genome annotated, and published with relevant data.