Publications by authors named "Ligia Maria Goncalves Fernandes"
Article Synopsis
- The study optimized the production of collagenase and protease enzymes from Aspergillus heteromorphus URM0269 using submerged fermentation with soybean flour as the primary substrate, achieving maximum enzyme production under specific concentrations of substrate and yeast extract.
- After scaling up the fermentation process in a stirred tank bioreactor, collagenase activity significantly increased while protease activity decreased, highlighting a shift in enzyme production dynamics.
- The research found that collagenase functions best at 40°C and pH 6.0, indicating its potential use in biotechnology, and also provided thermodynamic parameters that suggest the feasibility of using soybean flour as a sustainable resource for enzyme production.
This study aimed to produce, characterize and purify a protease from Aspergillus heteromorphus URM0269. After production by solid fermentation of wheat bran performed according to a central composite design, protease was characterized in terms of biochemical, kinetic, and thermodynamic parameters for further purification by chromatography. Proteolytic activity achieved a maximum value of 57.
View Article and Find Full Text PDFCollagenolytic proteases produced by URM0269 were extracted using a PEG/sulfate aqueous two-phase system (ATPS). A 2 factorial design was performed to analyze the independent variables: PEG molar mass (M), PEG concentration (C), and sulfate concentration (C). The extracted proteases were also evaluated for their optimum pH and stability at different pH levels (4.
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