Publications by authors named "Ligia Cosentino Junqueira Franco Spegiorin"
Identification and validation of reference genes of circulating microRNAs for use as control in gestational toxoplasmosis.
Mol Biochem Parasitol
December 2023
Toxoplasmosis causes serious harm to the fetus, as tachyzoite dissemination, during pregnancy in women developing the primo-infection. The microRNAs (miRNAs) are small non-coding RNAs, which have regulatory roles in cells by silencing messenger RNA. Circulating miRNA are promising biomarkers for diagnosis and prognosis of numerous diseases.
View Article and Find Full Text PDFLack of association of the KIR and HLA class I ligands with ZIKV infection in south and southeast of Brazil.
Mem Inst Oswaldo Cruz
August 2022
Background: Zika virus (ZIKV) is an emerging arbovirus associated with foetal malformations and neurological complications. The infection is usually associated with mild symptoms. The comparison between the allelic frequency of polymorphic genes in symptomatic infected individuals in the population can clarify the pathogenic mechanisms of ZIKV.
View Article and Find Full Text PDFTNFα rs1799964 TT genotype may be a susceptibility factor for vertical transmission of Toxoplasma gondii and clinical signs in newborns from pregnant women with acute toxoplasmosis.
Mol Biol Rep
June 2022
Background: One of the main impacts of Toxoplasma gondii infection occurs during pregnancy and is related to the vertical transmission of the parasite (congenital toxoplasmosis), which can cause severe clinical outcomes and fetal death. During acute infection, in order to control the rapid replication of tachyzoites, different host immune response genes are activated, and these include cytokine-encoding genes. Considering that polymorphisms in cytokine genes may increase susceptibility to vertical transmission of T.
View Article and Find Full Text PDFArticle Synopsis
- The study focused on analyzing extracellular vesicles (EVs), specifically microvesicles and exosomes, found in serum and cerebrospinal fluid (CSF) of patients affected by cerebral or gestational toxoplasmosis.
- Researchers examined clinical samples from 83 individuals divided into four groups based on their toxoplasmosis status and HIV infection to compare concentrations and characteristics of EVs.
- Findings revealed that serum-derived EVs from toxoplasmosis patients had significantly higher concentrations compared to healthy individuals, while the size of EVs was consistent across groups, indicating concentration rather than size could differentiate toxoplasmosis patients from non-infected individuals.
Molecular diagnosis of symptomatic toxoplasmosis: a 9-year retrospective and prospective study in a referral laboratory in São Paulo, Brazil.
Braz J Infect Dis
January 2018
Article Synopsis
- Symptomatic toxoplasmosis affects about 10-20% of those infected, posing significant public health issues, especially in Brazil.
- This study aimed to enhance the molecular diagnosis of symptomatic toxoplasmosis by comparing the efficacy of two primer sets (B1 and REP-529) in detecting Toxoplasma gondii DNA through real-time PCR in 807 clinical samples.
- The results showed that REP-529 was more sensitive, detecting T. gondii DNA in 97.23% of positive samples, compared to 78.80% for B1, thereby supporting its use as a more effective diagnostic tool.
Evaluation of serological and molecular tests used to identify Toxoplasma gondii infection in pregnant women attended in a public health service in São Paulo state, Brazil.
Diagn Microbiol Infect Dis
September 2017
Article Synopsis
- - Toxoplasmosis can seriously affect pregnant women, so accurate diagnosis is crucial using various testing methods.
- - A study compared serological tests (ELISA and ELFA) and different PCR techniques (cPCR, Nested PCR, qPCR) in pregnant women, both with and without symptoms of the disease.
- - The study found that while ELISA and ELFA showed similar results, ELFA detected more positive cases; however, molecular methods like cPCR had better sensitivity but lower specificity compared to others.
Introduction: Toxoplasmosis during pregnancy can be severe; thus, it is essential to diagnose the disease via serological tests.
Methods: An enzyme-linked immunosorbent assay (ELISA) was used to investigate anti-Toxoplasma gondii immunoglobulin A (IgA), M (IgM) and G (IgG) antibodies in 62 high-risk pregnant women.
Results: Forty-three (69.
Gestational Toxoplasma gondii infection is considered a major risk factor for miscarriage, prematurity and low birth weight in animals. However, studies focusing on this topic in humans are scarce. The objective of this study is to determine whether anti-Toxoplasma gondii maternal serum profiles correlate prematurity and low birth weight in humans.
View Article and Find Full Text PDFThe ADA*2 allele of the adenosine deaminase gene (20q13.11) and recurrent spontaneous abortions: an age-dependent association.
Clinics (Sao Paulo)
June 2012
Objective: Adenosine deaminase acts on adenosine and deoxyadenosine metabolism and modulates the immune response. The adenosine deaminase G22A polymorphism (20q.11.
View Article and Find Full Text PDFAnti-Toxoplasma gondii antibodies in pregnant women and their newborn infants in the region of São José do Rio Preto, São Paulo, Brazil.
Sao Paulo Med J
January 2012
Context And Objective: Toxoplasmosis transmission during pregnancy can cause severe sequelae in fetuses and newborns. Maternal antibodies may be indicators of risk or immunity. The aim here was to evaluate seropositivity for anti-Toxoplasma gondii (anti-T.
View Article and Find Full Text PDFSeropositivity rates for toxoplasmosis, rubella, syphilis, cytomegalovirus, hepatitis and HIV among pregnant women receiving care at a public health service, São Paulo state, Brazil.
Braz J Infect Dis
September 2011
Infectious and parasitic diseases affecting women during their reproductive age may result in vertical transmission. The aim of this study was to determine the seroprevalence for TORSCH among pregnant women receiving care at a university hospital. Records of 574 pregnant women who received medical attention from January 2006 to December 2007 were assessed.
View Article and Find Full Text PDFAim: This study aimed at evaluating the prevalence of high anticardiolipin antibody and lupus anticoagulant levels in women with histories of at least two miscarriages.
Methods: The presence of high anticardiolipin antibody and lupus anticoagulant levels were evaluated in 52 patients with ages between 17 and 41 (mean 26.7 years old) who had histories of repeated miscarriages.