Investigation of high-risk antibiotic resistance bacteria and their associated antibiotic resistance genes in different agricultural soils with biogas slurry from China.

High-risk antibiotic-resistant bacteria (ARB) and their accompanying antibiotic resistance genes (ARGs) seriously threaten public health. As a crucial medium for ARB and ARGs spread, soils with biogas slurry have been widely investigated. However, few studies focused on high-risk multi-drug resistant bacteria (MDRB) and their associated ARGs.

Effects of swine manure and straw biochars on fluorine adsorption-desorption in soils.

With increasing global awareness of soil health, attention must be paid to fluorine exposure in soils, which poses a threat to human health. Therefore, this study aimed to study the fluorine adsorption characteristics of swine manure and straw biochars and their impact on fluorine adsorption-desorption in soil with batch experiments. The biochar samples originated from high-temperature anaerobic cracking of swine manure (350°C, 500°C, and 650°C) and straw (500°C).

Persistent effects of swine manure biochar and biogas slurry application on soil nitrogen content and quality of lotus root.

Using swine manure biochar and biogas slurry in agriculture proves to be an effective strategy for soil improvement and fertilization. In this study, a pot trial on the growth of lotus root was conducted to investigate the persistent effects of applying 350°C swine manure biochar (1% and 2%) and biogas slurry (50% and 100%) on soil nitrogen nutrient and lotus root quality. The results showed that compared to chemical fertilizer alone (A0B0), swine manure biochar significantly increased soil nitrogen content after one year of application.

Hmgcs2 is the hub gene in diabetic cardiomyopathy and is negatively regulated by Hmgcs2, promoting high glucose-induced cardiomyocyte injury.

Background: Diabetic cardiomyopathy (DCM) represents a major cause of heart failure and a large medical burden worldwide. This study screened the potentially regulatory targets of DCM and analyzed their roles in high glucose (HG)-induced cardiomyocyte injury.

Methods: Through GEO database, we obtained rat DCM expression chips and screened differentially expressed genes.

Pseudolaric acid B triggers ferritinophagy and ferroptosis via upregulating NCOA4 in lung adenocarcinoma cells.

Background: Ferroptosis is a novel subtype of programmed cell death caused by iron-dependent lipid peroxidation and excessive reactive oxygen species (ROS) production. Small-molecule ferroptotic drugs have the probability of selectively targeting the specific features of aggressive tumor cells. In particular, pseudolaric acid B (PAB) triggered ferroptosisin breast cancer cells.

Responses of aquatic vegetables to biochar amended soil and water environments: a critical review.

Aquatic vegetables, including lotus root, water spinach, cress, watercress and so on, have been cultivated as commercial crops for a long time. Though aquatic vegetables have great edible and medicinal values, the increasing demands for aquatic vegetables with high quality have led to higher requirements of their soil and water environments. Unfortunately, the soil and water environment often face many problems such as nutrient imbalance, excessive fertilization, and pollution.

Comparison of Biochemical Parameters between Mouse Model and Human after Paraquat Poisoning.

Background: This study was designed to investigate differences in biochemical parameters between mouse and humans after paraquat (PQ) poisoning and develop a suitable animal model for studying organ damage after PQ poisoning. The prognostic factors of PQ-poisoned patients were further analyzed.

Methods: Thirty C57BL/6J mice were randomly divided into five groups (control, sham, and 3 PQ doses), and the mouse model was established by intragastric administration of PQ.

Effects of Biochar Application on Enzyme Activities in Tea Garden Soil.

Animal-manure biochar used as a sustainable amendment to garden soil has been widely applied, and the animal-manure pyrolysis temperatures would also have a regulatory effect on soil functions because of their affections on biochar physio-chemical properties. Here we studied the effects of different dosages of swine-manure biochar on tea garden soil functions, with the swine-manure pyrolysis temperature differed at 350 and 500°C. The results showed that the improvement of soil microbial biomass carbon and nitrogen and enzyme activities was closely related to the addition of 0.

Long-term biogas slurry application increased antibiotics accumulation and antibiotic resistance genes (ARGs) spread in agricultural soils with different properties.

Animal manures are commonly applied to soil which possibly promote the spread of antibiotic resistance from soil to human beings via food chains. Biogas slurry is an end product of anaerobic digestion of animal manures, which has been widely applied as fertilizers in the agricultural soil. However, effect of long-term biogas slurry application on the soil antibiotic resistance and the associated mechanism still remains unclear.

Performance, mechanism and stability of nitrogen-doped sewage sludge based activated carbon supported magnetite in anaerobic degradation of coal gasification wastewater.

In current study, the UASB reactor was enhanced by nitrogen-doped sewage sludge based activated carbon supported FeO (FeO/N-SBAC) for coal gasification wastewater treatment. The results showed that COD removal efficiency was increased to 64.4% with FeO/N-SBAC assistance and the corresponding methane production rate achieved up to 1093.

New insights into pollutants removal, toxicity reduction and microbial profiles in a lab-scale IC-A/O-membrane reactor system for paper wastewater reclamation.

In this study, an internal circulation-anoxic/aerobic (IC-A/O) process followed by ultrafiltration (UF) and reverse osmosis (RO) system was applied for paper wastewater reclamation. The IC-AO system presented a stable and efficient performance, achieving high removal of chemical oxygen demand (COD), total organic carbon (TOC) and total nitrogen (TN) with methane production rate of 132.8 mL/d.

Phenanthrene adsorption on soils from the Yangtze River Delta region under different pH and temperature conditions.

The phenanthrene (PHE) adsorption on soils from the Yangtze River Delta region under different pH and temperature conditions was studied in the laboratory. Results showed that the sorption of PHE on all soils was nonlinear and fitted to the Freundlich isotherm. The PHE adsorption on the soils is related to the content of organic carbons and the environmental conditions.

Enhancement of quality retention of fruiting bodies by erythorbic acid treatment.

In this paper, the effects of erythorbic acid (EA) treatment with different concentrations on the quality of fruiting bodies stored at 4 °C for 27 days were studied by determining the changes in moisture content, weight loss, browning, electrolyte leakage, malondialdehyde (MDA), and nutritional compounds. The activities of polyphenoloxidase (PPO), cellulase and other antioxidant enzymes including superoxide dismutase (SOD), catalase, and peroxidase (POD) were also measured. Results showed that 0.

Biochar reduces cadmium accumulation in rice grains in a tungsten mining area-field experiment: effects of biochar type and dosage, rice variety, and pollution level.

Cadmium (Cd)-contaminated rice (Oryza sativa) in Southern China is a great threat to food security, and the paddy soil remediation is urgently needed to reduce Cd accumulation in rice. Application of biochar could effectively immobilize soil Cd and reduce Cd uptake by rice. Fields that were applied with soil treatments including control and 15 and 30 t ha each hickory nut shell-derived biochar (KC) or maize straw-derived biochar (MC), and grown with two rice varieties (hybrid rice and late japonica rice) were selected for this study.

Production and characterization of a novel acidophilic and thermostable xylanase from Thermoascus aurantiacu.

The thermostable fungus, Thermoascus aurantiacus M-2, which produces a novel acidophilic and thermostable xylanase was isolated and identified based on its morphology and comparison of the internal transcribed spacer rDNA gene sequence. The culture conditions and components of medium were optimized for T. aurantiacus M-2 to produce xylanase.

Characterization and application of a newly isolated pyrene-degrading bacterium, .

PL5 (PL5) was newly isolated from soil sample and was identified by 16S rDNA sequence analysis. The strain PL5 had a high potential to degrade pyrene (PYR) in both liquid solution and soil and was able to degrade 51.8% of PYR at 25 °C and pH 7.

Improvement of extracellular lipase production by a newly isolated Yarrowia lipolytica mutant and its application in the biosynthesis of L-ascorbyl palmitate.

Yarrowia lipolytica Wt-11 producing an extracellular lipase was isolated and identified. To improve the lipase production, Y. lipolytica Wt-11 was subjected to low-energy ion implantation mutation breeding, and a best mutant, Y.

Application and comparison in biosynthesis and biodegradation by Fusarium solani and Aspergillus fumigatus cutinases.

In this study, two synthesized cutinase genes from Fusarium solani and Aspergillus fumigatus were expressed in Pichia pastoris X33. The characteristics of these two cutinases were investigated and compared. The results indicated that F.

Biodegradation of pyrene and benzo[a]pyrene in the liquid matrix and soil by a newly identified Raoultella planticola strain.

In the current study, the PL7 strain was isolated from soil and identified as Raoultella planticola based on its physiological characteristics and 16S rDNA sequence. By the 10th day, the PL7 strain degraded 52.0% of the pyrene (PYR) content and 50.

Protective effect of taraxasterol against rheumatoid arthritis by the modulation of inflammatory responses in mice.

Taraxasterol is an effective component of dandelion that has anti-inflammatory effects and . The present study was performed to explore whether taraxasterol exhibits a protective effect against rheumatoid arthritis through the modulation of inflammatory responses in mice. Eight-week-old CCR9-deficient mice were injected with a collagen II monoclonal antibody cocktail to create a rheumatoid arthritis model.

Glutathione protects against hepatic injury in a murine model of primary Sjögren's syndrome.

Primary Sjögren's syndrome (pSS) is a systemic autoimmune disease which may cause complications such as hepatic dysfunction and injury. As an important antioxidant, reduced glutathione (GSH) has been reported protecting against hepatic injury induced by some diseases, but the role of GSH in pSS is poorly understood. This study aims at investigating the role of GSH in hepatic injury during pSS.

Grifola frondosa Glycoprotein GFG-3a Arrests S phase, Alters Proteome, and Induces Apoptosis in Human Gastric Cancer Cells.

GFG-3a is a novel glycoprotein previously purified from the fermented mycelia of Grifola frondosa with novel sugar compositions and protein sequencing. The present study aims to investigate its effects on the cell cycle, differential proteins expression, and apoptosis of human gastric cancer SGC-7901 cells. Our findings revealed that GFG-3a induced the cell apoptosis and arrested cell cycle at S phase.

Performance of three microalgal strains in biogas slurry purification and biogas upgrade in response to various mixed light-emitting diode light wavelengths.

The three microalgal strains were cultivated, namely, Chlorella vulgaris, Scenedesmus obliquus, and Neochloris oleoabundans, by applying mixed light-emitting diode wavelength treatments to biogas slurry in a photobioreactor bag. This study aims to compare the growth and nutrient removal efficiency of the algae and determine their roles for biogas upgrading. At red:blue=5:5, S.

Hericium erinaceus polysaccharide-protein HEG-5 inhibits SGC-7901 cell growth via cell cycle arrest and apoptosis.

HEG-5 is a novel polysaccharide-protein purified from the fermented mycelia of Hericium erinaceus CZ-2. The present study aims to investigate the effects of HEG-5 on proliferation, cell cycle and apoptosis of human gastric cancer cells SGC-7901. Here, we first uncover that HEG-5 significantly inhibited the proliferation and colony formation of SGC-7901 cells by promoting apoptosis and cell cycle arrest at S phase.

Changes in chemical components and cytotoxicity at different maturity stages of Pleurotus eryngii fruiting body.

The present study investigated the changes of the chemical components and cytotoxicity potency at 5 developmental stages of Pleurotus eryngii fruiting body. The carbohydrate and protein contents increased along the maturity of fruiting body while fat content decreased. By comparison, the polysaccharide-protein fractions had the highest antiproliferative effect on SGC-7901 and HepG-2 cells in vitro and increasing activity with growing maturity of P.