Transcriptional landscape of sweetpotato root tip development at the single-cell level.

Single-cell transcriptome sequencing (scRNA-seq) is a powerful tool for describing the transcriptome dynamics of plant development but has not yet been utilized to analyze the tissue ontology of sweetpotato. This study established a stable method for isolating single protoplast cells for scRNA-seq to reveal the cell heterogeneity of sweetpotato root tip meristems at the single-cell level. The study analyzed 12,172 single cells and 27,355 genes in the root tips of the sweetpotato variety Guangshu 87, which were distributed into 15 cell clusters.

Determination of Cannabinoids in Meat Products and Animal Feeds in Singapore Using Liquid Chromatography-Tandem Mass Spectrometry.

There has been a growing interest in the use of hemp as an animal feed ingredient considering its economic value and nutritional properties. However, there is a paucity of research regarding the safety of hemp-based animal feed currently. Thus, this raises safety concerns on the potential transfer of cannabinoids from hemp-based animal feed to animal products intended for human consumption and its health effects.

Investigation of shared genetic features and related mechanisms between diabetes and tuberculosis.

Objective: This study aimed to integrate bioinformatics technology to explore shared hub genes and related mechanisms between diabetes and tuberculosis and to provide a theoretical basis for revealing the disease mechanisms in patients with both diabetes and tuberculosis.

Methods: Differentially expressed genes and Venn analysis were used to identify shared genes between diabetes and tuberculosis. PPI network analysis was used to screen key hub genes.

Surveillance of veterinary drug residues in food commonly consumed in Singapore and assessment of dietary exposure.

Non-judicious and indiscriminate use of veterinary drugs in animal husbandry may result in accumulation of residues in animal tissues, and consequently in food for human consumption. The abuse of veterinary drugs presents a potential risk to consumer health, especially if the residue level is higher than the health-based guidance value (HBGV) such as the acceptable daily intake (ADI). Contamination of drug residues in food also promotes the emergence of antimicrobial resistance (AMR) which poses a serious threat to public health globally.

Advance Progress in Assembly Mechanisms of Carrier-Free Nanodrugs for Cancer Treatment.

Nanocarriers have been widely studied and applied in the field of cancer treatment. However, conventional nanocarriers still suffer from complicated preparation processes, low drug loading, and potential toxicity of carriers themselves. To tackle the hindrance, carrier-free nanodrugs with biological activity have received increasing attention in cancer therapy.

Genetic fingerprint construction and genetic diversity analysis of sweet potato (Ipomoea batatas) germplasm resources.

Background: China is the largest producer of sweet potato in the world, accounting for 57.0% of the global output. Germplasm resources are the basis for promoting innovations in the seed industry and ensuring food security.

3D printed hollow channeled hydrogel scaffolds with antibacterial and wound healing activities.

The development of hydrogel based scaffold with the capability of enhanced antibacterial effects and wound healing is the promising strategy for the treatment of wound tissues with bacterial infection. Herein, we fabricated a hollow channeled hydrogel scaffold based on the mixture of dopamine modified alginate (Alg-DA) and gelatin via co-axial 3D printing for the treatment of bacterial-infected wound. The scaffold was crosslinked by copper/calcium ions, which could enhance the structural stability and mechanical properties.

Dual Coordination between Stereochemistry and Cations Endows Polyethylene Terephthalate Fabrics with Diversiform Antimicrobial Abilities for Attack and Defense.

Modification of fabrics by stereochemical antiadhesion strategies is an emerging approach to antimicrobial fabric finishing. However, a purely antiadhesive fabric cannot avoid the passive adhesion of pathogenic microorganisms. To address this issue, borneol 4-formylbenzoate (BF) with a stereochemical structure is introduced into a cationic polymer PEI-modified PET fabric by a simple two-step method.

Multicomponent carrier-free nanodrugs for cancer treatment.

Nanocarriers can be used to deliver insoluble anticancer drugs to optimize therapeutic efficacy. However, the potential toxicity of nanocarriers cannot be ignored. Carrier-free nanodrugs are emerging safe drug delivery systems, which are composed of multiple components, such as drugs, bioactive molecules and functional ingredients, avoiding the usage of inert carrier materials and offering advantages that include high drug loading, low toxicity, synergistic therapy, versatile design, and easy surface functionalization.

The complete mitochondrial genome of the jumping plant bug Reuter, 1885 (Hemiptera: Miridae).

The complete mitochondrial genome of was sequenced and analyzed in this study. The mitochondrial genome is 15,403 bp in size and comprises 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and one control region (D-loop). The nucleotide composition of the mitogenome is 41.

Exploration of molecular mechanism of intraspecific cross-incompatibility in sweetpotato by transcriptome and metabolome analysis.

Cross-incompatibility, frequently happening in intraspecific varieties, has seriously restricted sweetpotato breeding. However, the mechanism of sweetpotato intraspecific cross-incompatibility (ICI) remains largely unexplored, especially for molecular mechanism. Treatment by inducible reagent developed by our lab provides a method to generate material for mechanism study, which could promote incompatible pollen germination and tube growth in the ICI group.

The mitochondrial genome of the endophyte CRI Eg3 isolated from sweet potato.

CRI Eg3 was isolated from the leaves of sweet potato. Its complete mitogenome contains 37,226 bp, with a G + C content of 26.1%.

Combating Biofilms by a Self-Adapting Drug Loading Hydrogel.

A bacterial biofilm is one of the main reasons that many diseases are difficult to cure. Herein, a teicoplanin (TPN)-loaded self-adapting chitosan-based hydrogel (CPH) system, called TPN-CPH, was prepared by encapsulating antibacterial TPN into CPH. This TPN-CPH can effectively combat preformed biofilms of ().

Inserting Menthoxytriazine into Poly(ethylene terephthalate) for Inhibiting Microbial Adhesion.

Antimicrobial modification of poly(ethylene terephthalate) (PET) is effective in preventing the adhesion and growth of microorganisms on its surface. However, few methods are available to modify PET directly at its backbone to impart the antimicrobial effect. Herein, menthoxytriazine-modified PET (PMETM) based on the stereochemical antimicrobial strategy was reported.

Genome Resource for , the Causal Agent of Stem and Foliage Scab Disease of Sweet Potato.

is a phytopathogenic fungus causing stem and foliage scab disease of sweet potato. At present, there is no reference genome available for , limiting basic research for the pathogen. The present study applied the Nanopore single-molecule sequencing technology to sequence the genome.

Draft Genome Sequence of Causing Dry Rot Disease in Sweetpotato.

Dry rot caused by leads to the serious decay of sweetpotato storage roots during postharvest storage, which can result in considerable economic loss. Genomic research of the pathogen could provide a basis for study and prevention of sweetpotato dry rot. Herein, we report a high-quality draft genome sequence of CRI 302-4 isolated from infected sweetpotato storage roots in Taizhou City, Zhejiang Province, China.

Preoperative Prediction of Cytokeratin 19 Expression for Hepatocellular Carcinoma with Deep Learning Radiomics Based on Gadoxetic Acid-Enhanced Magnetic Resonance Imaging.

Purpose: Cytokeratin 19 (CK19) expression is a proven independent prognostic predictor of hepatocellular carcinoma (HCC). This study aimed to develop and validate the performance of a deep learning radiomics (DLR) model for CK19 identification in HCC based on preoperative gadoxetic acid-enhanced magnetic resonance imaging (MRI).

Patients And Methods: A total of 141 surgically confirmed HCCs with preoperative gadoxetic acid-enhanced MRI from two institutions were included.

MiR-144-3p inhibits the proliferation, migration and invasion of lung adenocargen cancer cells by targeting COL11A1.

This study aimed to investigate the regulatory relationship between miR-144-3p and COL11A1, and to explore its effect on the proliferation, migration and invasion of lung adenocarcinoma (LUAD) cells. A series of methods and experiments were applied. miR-144-3p was downregulated in LUAD tissue and cells, whereas COL11A1 was highly expressed.