Identification of core immune-related genes CTSK, C3, and IFITM1 for diagnosing Helicobacter pylori infection-associated gastric cancer through transcriptomic analysis.

Objectives: To identify diagnostic genes and mechanisms linking Helicobacter pylori (H. pylori) infection to gastric cancer.

Methods: Gene expression profiles from GEO were analyzed using differential expression gene (DEG) analysis, weighted gene co-expression network analysis (WGCNA), and functional enrichment.

[Viable Bacteria Assay of by RT-qPCR Measurement of Gene Expression Levels: Establishment and Application of a New Method].

Objective: To establish a viable bacteria assay for () by assessing the gene expression, and to develop accordingly a rapid and novel testing method for clinical precision treatment.

Methods: Viable bacteria count was determined in bacterial cultures. The transcriptional expression level of (), the conserved gene that encodes cholesterol-α-glucosyltransferase (CGT) in , was measured by RT-PCR.

[Mechanisms of Intracellular Infection and Reflections Concerning Clinical Practice].

(), for a long time, has generally been considered an extracellular bacterium. However, recent findings have shown that can gain entry into host cells, evade attacks from the host immune system and the killing ability of medication, form stable intracellular ecological niche, and achieve re-release into the extracellular environment, thus causing recurrent infections. intracellular infection causes cellular signaling and metabolic alterations, which may be closely associated with the pathogenesis and progression of tumors, thereby presenting new challenges for clinical eradicative treatment of .

Inhibitory and Injury-Protection Effects of O-Glycan on Gastric Epithelial Cells Infected with Helicobacter pylori.

Helicobacter pylori (H. pylori) is an important pathogen that can cause gastric cancer. Multiple adhesion molecules mediated H.

[Sensitivity Comparison Experiment of Four Testing Methods for ].

Objective: To measure with standard microbiology methods the sensitivity of 4 commonly used testing methods for (Hp) and to conduct a comparative study of the correlations and differences across the 4 methods.

Methods: With the Hp standard strain (SS1) as the reference, colony forming units (CFU) as the units of quantitative analysis for detection performance, and gradient dilution of SS1 suspension as the simulation sample, we measured the sensitivity of 4 Hp testing methods, including bacterial culture, rapid urease test, antigen test, and quantitative fluorescent PCR. CFU values at different concentrations corresponding to the 4 commonly used Hp testing methods were documented and the correlations and differences were analyzed accordingly.