Inhibition of D-glyceraldehyde-3-phosphate dehydrogenase by ATP and quinaldate.

The inhibition of D-glyceraldehyde-3-phosphate dehydrogenase by ATP is of purely mixed type with respect to NAD (Ki=4.9 mM), purely uncompetitive with respect to D-glyceraldehyde-3-phosphate (Ki=9.4 mM) and partially uncompetitive with respect to inorganic phosphate (Ki=6.

pH and temperature dependence of the double inhibition of D-glyceraldehyde-3-phosphate dehydrogenase by ATP and quinaldate.

ATP and quinaldate, two inhibitors of D-glyceraldehyde-3-phosphate dehydrogenase, act antagonistically if [Pi] greater than 1 mM, at pH 7.5 and 25 degrees C. However, the type of interaction of the two inhibitors changes with pH and temperature.