Publications by authors named "Lie-Jun Li"
Article Synopsis
- The study focuses on the α-globin fusion gene, crucial for thalassemia screening, which can lead to severe conditions when paired with α-thalassemia.
- Researchers analyzed blood samples suspected of containing this gene in southern China, confirming 266 cases from 2017 to 2023, primarily in Hainan province and Guangzhou.
- Findings indicate that while carriers of the fusion gene may not show symptoms, individuals with the α-globin fusion gene (αα)/ experience anemia, highlighting its prevalence in the Li minority and the need for genetic counseling.
Article Synopsis
- HPV is the leading cause of cervical cancer, and this study aimed to explore the effectiveness of using urine samples alongside genital samples for detecting HPV DNA and genotyping.
- The study included 250 patients from a hospital in Guangdong, China, and found a 77.1% agreement in HPV detection between paired samples in females, indicating that urine could serve as a reliable substitute for genital samples in women.
- Results showed that while urine detection correlates well with high-risk HPV types in females, its effectiveness in males is uncertain, suggesting a need for further research regarding male HPV detection methods.
Thalassemia presents a higher incidence in southern China. The objective of this study is to analyze the genotype distribution of thalassemia in Yangjiang, a western city of Guangdong Province in China. The genotypes of suspected cases with thalassemia were tested by PCR and reverse dot blot (RDB).
View Article and Find Full Text PDFObjective: To evaluate a novel reverse dot blot assay for the simultaneous detection six types of common α-thalassaemia alleles (three deletional and three common non-deletional mutations) and 19 types of common β-thalassaemia alleles in a Chinese population.
Methods: Genomic DNA samples were collected from three hospitals in southern China. The novel thalassaemia gene assay involved one multiplex polymerase chain reaction amplification system and one round of hybridization.
Reverse Transcription Recombinase-Aided Amplification Assay With Lateral Flow Dipstick Assay for Rapid Detection of 2019 Novel Coronavirus.
Front Cell Infect Microbiol
February 2021
Background: The emerging Coronavirus Disease-2019 (COVID-19) has challenged the public health globally. With the increasing requirement of detection for SARS-CoV-2 outside of the laboratory setting, a rapid and precise Point of Care Test (POCT) is urgently needed.
Methods: Targeting the nucleocapsid (N) gene of SARS-CoV-2, specific primers, and probes for reverse transcription recombinase-aided amplification coupled with lateral flow dipstick (RT-RAA/LFD) platform were designed.
Article Synopsis
- The study presents a new multiplex real-time PCR-based assay for detecting high-risk HPV types in China, highlighting its effectiveness in identifying HPV 16 and 18.
- The assay is designed for both clinical settings and large-scale cervical screening, showcasing its sensitivity and reliability.
- This innovative and cost-effective approach aims to alleviate the economic burden of HPV detection in the country.
Article Synopsis
- HPV infection is the main cause of cervical cancer, with a study conducted on rural women in Chaozhou, China, revealing a 7.89% high-risk HPV prevalence among 48,559 cases.
- The study identified age (55-60 years) as a peak incidence group, and found that the most common HPV types were 52, 16, and 58, with a significant portion (48.23%) of infections involving types 52 and 58 combined.
- Results suggest a low prevalence of HPV vaccine types 16 and 18, indicating the potential benefit of developing second-generation HPV vaccines targeting types 52 and 58 for better protection in this rural population.
Genital human papillomavirus screening by gene chip in Chinese women of Guangdong province.
Aust N Z J Obstet Gynaecol
April 2008
Background: Human papillomavirus (HPV) infections are associated with cervical cancer. There were only a few reports and detailed data about epidemiological research of HPV infection in general population of China.
Aims: To determine the prevalence of genital HPV infection in Chinese women of Guangdong province.