Publications by authors named "Lidiya Franklin"
Members of the piggyBac superfamily of DNA transposons are widely distributed in host genomes ranging from insects to mammals. The human genome has retained five piggyBac-derived genes as domesticated elements although they are no longer mobile. Here, we have investigated the transposition properties of piggyBat from Myotis lucifugus, the only known active mammalian DNA transposon, and show that its low activity in human cells is due to subterminal inhibitory DNA sequences.
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- - The study explores CRISPR-Cas12a as an effective genome editing tool for AT-rich regions, specifically tested on rice, by screening nine different orthologs and discovering six with high editing activity.
- - Among the identified variants, Mb2Cas12a is highlighted for its impressive editing efficiency and performance at low temperatures, and an engineered variant (Mb2Cas12a-RVRR) allows for broader PAM targeting.
- - A comparison of 12 multiplexed Cas12a systems reveals a highly effective method capable of nearly 100% biallelic editing across up to 16 sites in rice, setting a new record for plant genome editing using this technology.