Direct microbial production of 2,3-Butanediol from inulin by Bacillus velezensis R22 through the synergistic action of membrane and cytoplasmic inulinases.

The present study investigates the natural ability of Bacillus velezensis R22 to produce 2,3-BD from two inulin-rich substrates - insoluble and soluble chicory flour. After complex optimization of the media content and process parameters by consecutive application of Plackett-Burman design and response surface methodology, the strain R22 was capable of producing 71.2 g/L (95.

Bacterial Tolerance to 1-Butanol and 2-Butanol: Quantitative Assessment and Transcriptomic Response.

The unique fuel characteristics of butanol and the possibility of its microbial production make it one of the most desirable environmentally friendly substitutes for petroleum fuels. However, the highly toxic nature of 1-butanol to the bacterial strains makes it unprofitable for commercial production. By comparison, 2-butanol has similar fuel qualities, and despite the difficulties in its microbial synthesis, it holds promise because it may be less toxic.

Inactivation of Gene Allows Higher 2,3-Butanediol Production by from Inulin.

24 (BL24) is an efficient, non-pathogenic producer of 2,3-butanediol (2,3-BD). However, during inulin fermentation, the strain produces large amounts of exopolysaccharides (EPS), which interfere with the process' performance. The present study aims to investigate the effect that inactivation of the gene, encoding levansucrase in BL24, has on 2,3-BD production efficiency.

Whole-genome sequence of strain R22 isolated from rhizosphere in Bulgaria.

R22 was isolated from a rice rhizosphere in Bulgaria. Its genome (assembled into 14 scaffolds) has a size of 4.08 Mbp and a G + C content of 46.

Influence of pH on Inulin Conversion to 2,3-Butanediol by 24: A Gene Expression Assay.

2,3-Butanediol (2,3-BD) is an alcohol highly demanded in the chemical, pharmaceutical, and food industries. Its microbial production, safe non-pathogenic producer strains, and suitable substrates have been avidly sought in recent years. The present study investigated 2,3-BD synthesis by the GRAS 24 using chicory inulin as a cheap and renewable substrate.

Microbial Detoxification of Residual Pesticides in Fermented Foods: Current Status and Prospects.

The treatment of agricultural areas with pesticides is an indispensable approach to improve crop yields and cannot be avoided in the coming decades. At the same time, significant amounts of pesticides remain in food and their ingestion causes serious damage such as neurological, gastrointestinal, and allergic reactions; cancer; and even death. However, during the fermentation processing of foods, residual amounts of pesticides are significantly reduced thanks to enzymatic degradation by the starter and accompanying microflora.

High production of acetoin from glycerol by Bacillus subtilis 35.

Acetoin is a high-value volatile compound widely applied in the chemical, food, and pharmaceutical industries. Despite the promising use of waste glycerol as a substrate in several microbial syntheses, acetoin production by natural microorganisms from glycerol as a sole carbon source has never been reported. The present study investigates the innate ability of Bacillus subtilis 35 (DSM 113,620) to convert glycerol into acetoin and 2,3-butanediol.

In Vitro Production of Galactooligosaccharides by a Novel β-Galactosidase of .

β-galactosidase is an enzyme with dual activity and important industrial application. As a hydrolase, the enzyme eliminates lactose in milk, while as a trans-galactosidase it produces prebiotic galactooligosaccharides (GOS) with various degrees of polymerization (DP). The aim of the present study is the molecular characterization of β-galactosidase from a Bulgarian isolate, subsp.

The Complex Role of Lactic Acid Bacteria in Food Detoxification.

Toxic ingredients in food can lead to serious food-related diseases. Such compounds are bacterial toxins (Shiga-toxin, listeriolysin, Botulinum toxin), mycotoxins (aflatoxin, ochratoxin, zearalenone, fumonisin), pesticides of different classes (organochlorine, organophosphate, synthetic pyrethroids), heavy metals, and natural antinutrients such as phytates, oxalates, and cyanide-generating glycosides. The generally regarded safe (GRAS) status and long history of lactic acid bacteria (LAB) as essential ingredients of fermented foods and probiotics make them a major biological tool against a great variety of food-related toxins.

New Exopolysaccharides Produced by 24 Display Substrate-Dependent Content and Antioxidant Activity.

is a soil bacterium with many industrial applications. In addition to enzymes, platform chemicals, antibiotics and phytohormones, the species produces exopolysaccharides (EPSs) of various biological activities. This study revealed that Bulgarian isolate 24 produced EPSs consisting of galactose, glucose and mannose with substrate-dependent ratio.

Traditional Bulgarian Dairy Products: Ethnic Foods with Health Benefits.

The reported health effects of fermented dairy foods, which are traditionally manufactured in Bulgaria, are connected with their microbial biodiversity. The screening and development of probiotic starters for dairy products with unique properties are based exclusively on the isolation and characterization of lactic acid bacterial (LAB) strains. This study aims to systematically describe the LAB microbial content of artisanal products such as Bulgarian-type yoghurt, white brined cheese, , , , , and the Rhodope's .