Anodic aluminium oxide (AAO) membranes with self-ordered nanochannels have become promising candidates for applications in the aspects such as structural coloration, photonic crystals, upconversion luminescence and nanofluidic transport. Also, self-ordered AAO membranes have been extensively used for the fabrication of functional nanostructures such as nanowires, nanotubes, nanoparticles, nanorods and nanopillars. Geometries of nanochannels are crucial for the applications of AAO membranes as well as controlling growth (, nucleation, direction and morphology) and in applications (, optics, magnetics, thermoelectrics, biology, medicine, sensing, and energy conversion and storage) of the functional nanostructures fabricated AAO template-based methods.
View Article and Find Full Text PDFGlutamine, like glucose, is a major nutrient consumed by cancer cells, yet these cells undergo glutamine starvation in the cores of tumors, forcing them to evolve adaptive metabolic responses. Pharmacologically targeting glutamine metabolism or withdrawal has been exploited for therapeutic purposes, but does not always induce cancer cell death. The mechanism by which cancer cells adapt to resist glutamine starvation in cisplatin-resistant non-small-cell lung cancer (NSCLC) also remains uncertain.
View Article and Find Full Text PDFCell Mol Biol (Noisy-le-grand)
June 2020
The current experiment was performed to investigate the effect of LncRNA NORAD on the sensitivity of miR-410-3p to drug resistance of osteosarcoma cells. The cisplatin-resistant cell line HOS/DDP was induced; si-NC, si-NORAD, miR-NC, miR-410-3p, pcDNA-NC, and pcDNA-NORAD were transfected into HOS/DDP cells, respectively; record as a si-NC group, si-NORAD group, miR-NC group, miR-410-3p group, pcDNA-NC group, pcDNA-NORAD group; si-NORAD was co-transfected into HOS/DDP cells with anti-miR-NC, anti-miR-410-3p, recorded as an anti-miR-NC+si-NORAD group and anti-miR-410-3p+si-NORAD group. Real-time quantitative PCR (RT-qPCR) was used to detect LncRNA NORAD, miR-410-3p and multidrug resistance protein 1 (MRP1) mRNA expression levels; Western blot was used to detect cyclin D1 (CyclinD1), MRP1, phosphorylated (p-p65), phosphorylated IкBα (p-IкBα) protein expression; cell counting kit 8 (CCK-8) was used to detect cell viability; dual luciferase report assay to detect targeting relationship between LncRNA NORAD and miR-410-3p.
View Article and Find Full Text PDFPurpose: The main objectives of this study were to assess the early changes in pulmonary function and intrarenal haemodynamics and to determine the correlation between pulmonary function and intrarenal haemodynamics in patients with type 2 diabetes mellitus (T2DM).
Methods: 96 patients with T2DM (diabetes group) without diabetes kidney disease (DKD) and 33 healthy subjects (control group) were enrolled in studies intended to assess the early changes in pulmonary function and intrarenal haemodynamics associated with diabetes, as well as to determine the correlation between pulmonary function and intrarenal haemodynamics.
Results: Pulmonary functional parameters were negatively correlated with HbA1c levels and diabetes duration (P< 0.
Objective: The primary aim of this study is to examine the hemodynamics of retrobulbar and intrarenal in the changes of early stage of type 2 diabetes mellitus (T2DM) patients from 2000 to 2015 and to assess incidence associated with diabetic kidney disease (DKD) and diabetic retinopathy (DR).
Method: Our study contained 60 subjects newly diagnosed of T2DM were divided into 2 groups base on the mean resistive index (RI) (≤0.7 and >0.
Objective: To observe the effect of electroacupuncture (EA) of "Zusanli" (ST 36) on mitochondrial oxidative stress of skeletal muscle in rats with chronic fatigue syndrome (CFS) based on adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK)/ peroxlsome proliferator-activated receptor-γ coactivator-1 α (PGC-1 α) signaling, in order to reveal its mechanism underlying improvement of CFS.
Methods: Forty SD rats were randomly divided into normal control, CFS model, EA-Zusanli (ST 36) and EA-non-acupoint groups (=10 rats in each group). The CFS model was established by forced exhausted load-bearing swimming (twice daily), chronic constraint (1 h) and sleep deprivation (20 h/day) for 14 days.
Objective: To observe the expression change of mitophagy-related proteins in skeletal muscle in rats with spleen deficiency syndrome and to explain the partial action mechanism of acupuncture at Zusanli (ST 36) for spleen deficiency syndrome.
Methods: Forty male SD rats, after normal feeding, were randomly divided into a normal group, a spleen deficiency group, a Zusanli group and a non-acupoint group, ten rats in each group. Except the normal group, the three factors modeling method was used for 14 days to establish the model of spleen deficiency syndrome on the other 3 groups.
Hyperplasia of mammary glands (HMG) is also termed mammary dysplasia. In China, the number of patients suffering from breast hyperplasia is increasing annually. MicroRNAs (miRNAs; length, 19-24 nucleotides), a group of small endogenous non-coding RNAs, post-transcriptionally regulate gene expression via RNA interference and gene silencing pathways.
View Article and Find Full Text PDFAcupuncture was proven beneficial in treating allergic inflammation. We aimed to explore the regulation underlying the effects of acupuncture on Feishu, an acupoint most commonly used in the acupuncture therapy for respiratory diseases, with respect to the system of sympathetic nerve neurotransmitter acetylcholine (Ach). Male Wistar rats were randomly grouping.
View Article and Find Full Text PDFObjective: To observe the effect of electroacupuncture (EA) at "Zusanli"(ST 36) on Ghrelin/cAMP/PKA expression in the jejunum in rats with spleen deficiency syndrome, so as to reveal its underlying mechanism in improving energy metabolism.
Methods: Forty male SD rats were randomly divided into 4 groups:normal group, spleen deficiency syndrome (model) group, EA group and non-acupoint group (=10 in each group).The model of spleen deficiency syndrome was established by improper diet and overstrain.
To study the influence of astragaloside on mRNA expression of PI3K/Akt/mTOR signal transduction in anemia model mice induced by chemotherapy, 48 male BALB/c mice which were 6-7 week old were picked as the research objects and randomly divided into four groups, blank group, model group, astragaloside group and astragaloside IV group. Each group was 12 mice. Chemotherapy anemia model was established by cyclophosphamide.
View Article and Find Full Text PDFBruceantinol (BOL), a quassinoid compound isolated from the fruits of , has been reported to have cytotoxic and antibacterial effects. In this study, a rapid, sensitive, and specific ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method was developed for the quantitative determination of BOL in rat plasma. The samples were treated by simple liquid-liquid extraction with ethyl acetate and separated on an UPLC BEH C18 column (2.
View Article and Find Full Text PDFPlasmonic resonant cavities are capable of confining light at the nanoscale, resulting in both enhanced local electromagnetic fields and lower mode volumes. However, conventional plasmonic resonant cavities possess large Ohmic losses at metal-dielectric interfaces. Plasmonic near-field coupling plays a key role in a design of photonic components based on the resonant cavities because of the possibility to reduce losses.
View Article and Find Full Text PDFTuning transversal resonance modes of localized surface plasmons (LSPs) by the size and the ambient dielectric medium of Ag nanorods is presented. It is found that the resonance wavelength and intensity of the transversal modes of LSPs are closely related to the dimensions of the Ag nanorods embedded in anodic aluminum oxide membranes. The transversal resonance peak exhibits obvious redshifts from 365 to 396 nm with increasing nanorod diameter from 40 to 80 nm, and the resonance intensity remarkably enhances with increasing nanorod diameter.
View Article and Find Full Text PDFA number of epidemiological studies have established a link between Alzheimer's disease (AD) and diabetes mellitus (DM). So, nuclear receptor peroxisome proliferator-activated receptor gamma (PPARγ) plays an important role in the treatment of AD. However, current PPARγ-targeting drugs such as thiazolidinediones (TZDs) are associated with undesirable side effects.
View Article and Find Full Text PDFObjective: To investigate the impact of electro-acupuncture at the Neiguan (PC 6) acupoint on protein and RNA expression of acid-sensing ion channel 2 (ASIC2) and ASIC3 in myocardial ischemia rats.
Methods: Fifty male Sprague-Dawley rats were used, weighing (230 ± 50) g. The rats were randomized into a normal group A, model group B, Neiguan (PC 6) group C, Lieque (LU 7) group D, and A-shi points group E.
The cathodoluminescence and optoelectronic properties based on an individual CdS/CdSe/CdS biaxial nanobelt are revealed in the present study. Both typical CdS and CdSe emissions are detected from as-grown CdS/CdSe/CdS nanobelts. The photodetector based on this nanobelt exhibits high sensitivity and excellent cycle stability.
View Article and Find Full Text PDFIn Vitro Cell Dev Biol Anim
February 2015
This study aimed to investigate the direct effects of advanced glycation end products (AGEs) on the mitochondrial structure and function of SH-SY5Y cells and the possible molecular mechanism(s) underlying mitochondria dysfunction by AGEs. SH-SY5Y cells were cultured in 400 μg/ml of AGE-bovine serum albumin (BSA) for 24 h, and changes in the mitochondrial function of SH-SY5Y cells were analysed as follows. Reactive oxygen species (ROS) were detected using 2',7'-dichlorodihydrofluorescein diacetate molecular probes.
View Article and Find Full Text PDFNovel low-cost layered Fe-titanate catalyst for photo-Fenton degradation of organic contaminants was successfully developed by ion exchange of Fe(3+) with Na(+) layered nano Na-titanates which was prepared by alkali hydrothermal method. The as prepared materials were characterized by powder X-ray diffraction analysis (XRD), field emission scanning electron microscopy (FESEM) and energy-dispersive X-ray spectrometer (EDX). The catalytic activity of the Fe-titanate catalyst was evaluated by the decolorization of three different dyes (rhodamine 6G (R6G), methyl blue (MB), and methyl orange (MO)) under UV irradiation at room temperature.
View Article and Find Full Text PDFObjective: To explore mechanism of acupuncture for renal interstitial fibrosis (RIF) in hypertension rats.
Methods: Twenty-four 24-week-old male spontaneously hypertensive rats were randomly divided into a model group, a perindopril group and an acupuncture group, eight cases in each one. In the acupuncture group, with rats tied up, electroacupuncture was applied at bilateral "Quchi" (LI 11) and "Zusanli" (ST 36) under mild vibration of needle handle for 20 min, once a day.
ACS Appl Mater Interfaces
August 2012
Iron(III) oxide photoelectrodes show promise in water oxidation applications. In this study, micro-nano-structured hematite films are synthesized, and Ti ions are doped to improve photoelectric conversion efficiency. The photocurrent increases for enhanced electrical conductivity.
View Article and Find Full Text PDFA novel 1D/1D nanocomposite-based photodetector is successfully fabricated from high-crystalline ZnS/ZnO biaxial nanobelts for the first time. Optimized performance of the ZnS/ZnO nanobelt photodetector is much better than that of pure ZnS or ZnO nanostructures, with a wide-range UV-A light photoresponse, high sensitivity, and very fast response speed.
View Article and Find Full Text PDFObjective: To investigate the effect of the eye-acupuncture therapy on cerebral intercellular adhesion molecule-1 (ICAM-1) expression in rats with acute cerebral ischemia-reperfusion injury (CI/RI) so as to reveal its underlying mechanism.
Methods: Forty male SD rats were randomly and equally divided into normal, sham operation (sham), model and eye-acupuncture groups. Acute CI/RI model was established by occlusion of the middle cerebral artery with a modified suture-blocking method.
J Nanosci Nanotechnol
September 2011
In this paper, the nanostructured Bi2WO6 with different hierarchical morphologies was synthesized via a warmly hydrothermal route. The structure and morphology of the as-prepared Bi2WO6 products were characterized by X-ray diffraction (XRD), field emission scanning electron microscope (FE-SEM), UV-vis absorption spectroscopy (UV-Vis) and N2-sorption analysis. The photocatalytic efficiency of Bi2WO6 was investigated by photodegradation of rhodamine B (RhB) under visible-light irradiation.
View Article and Find Full Text PDFA facile chemical route is presented to synthesize ZnO nanoarrays including one-dimensional nanowire arrays and two-dimensional porous nanosheet arrays. Large-scale ZnO nanowire arrays with the length of 5 microm and aspect ratio of 42 were achieved by cyclic growth in aqueous solution. After being immerged in the zinc acetate solution for 24 h, the ZnO nanowire arrays converted to sheet-like Zn5(OH)8(CH3COO)2 arrays.
View Article and Find Full Text PDF