Publications by authors named "Lide Chen"

Dairy manure, a significant source of phosphorus (P), can potentially cause environmental risk due to P runoff when dairy manure is directly applied to cropland. Thus, there is an increasing interest in mitigating P loss from manure prior to land applications. This study aimed to investigate the potential of hydrochar produced by hydrothermal carbonization (HTC) for P recycling from dairy manure with and without the addition of CaO, focusing on the plant bioavailability, stabilization, and transformation of P in the resultant hydrochar.

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With increasing demand for meat and dairy products, the volume of wastewater generated from the livestock industry has become a significant environmental concern. The treatment of livestock wastewater (LWW) is a challenging process that involves removing nutrients, organic matter, pathogens, and other pollutants from livestock manure and urine. In response to this challenge, researchers have developed and investigated different biological, physical, and chemical treatment technologies that perform better upon optimization.

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The technological development for efficient nutrient removal from liquid dairy manure is critical to a sustainable dairy industry. A nutrient removal process using a two-step fed sequencing batch reactor (SBR) system was developed in this study to achieve the applicability of simultaneous removal of phosphorus, nitrogen, and chemical oxygen demand from anaerobically digested liquid dairy manure (ADLDM). Three operating parameters, namely anaerobic time:aerobic time (min), anaerobic DO:aerobic DO (mg L), and hydraulic retention time (days), were systematically investigated and optimized using the Taguchi method and grey relational analysis for maximum removal efficiencies of total phosphorus (TP), ortho-phosphate (OP), ammonia-nitrogen (NH-N), total nitrogen (TN), and chemical oxygen demand (COD) simultaneously.

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During anaerobic digestion (AD) of liquid dairy manure, organic nitrogen converts to ammonia nitrogen (NH-N) and subsequently escalates the NH-N concentrations in manure. Among different available NH-N removal processes treating anaerobically digested liquid dairy manure (ADLDM), vacuum thermal stripping is reported to be an effective technique. However, none of the studies have performed multi-parameter optimization, which is of utmost significance in maximizing process efficiency.

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Today, the livestock industry is considered to be one of the biggest emitters of ammonia in the world. The nitrogen present in livestock manure has been linked to the contamination of water bodies. Livestock manures contain a significant quantity of recoverable nitrogen.

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Duckweed has been recognized for its potential of producing biomass on nutrients from waste streams. Our research has shown that strains of duckweed can be successfully cultivated on anaerobically digested (AD) dairy manure under controlled levels of nitrogen (N) and phosphorus (P). The objective of this study was to explore the maximization of starch accumulation in Landoltia punctata (Mey.

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The non-airtight digestion technology is emerging to be applied in the acidogenic phase for two-stage methane production. However, in this study, it was used to pretreat screened dairy manure (SDM) in order to provide microalgae cultivation with a substrate that might be more suitable for nutrient reduction, especially phosphorus. SDM was firstly underwent non-airtight digestion applying different dilution folds, i.

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A number of dairies in southern Idaho employed stationary inclined screens to separate large solid particles out of liquid dairy manure. In this way, the total solid content of the liquid dairy manure can drop about 20%. Solids in dairy wastewater cause high turbidities, which could block the incident light, a key factor in the microalgae cultivation process using wastewaters as culture media.

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High concentrations of dairy operations in Southern Idaho have brought up big challenges for proper manure management to alleviate the eutrophication stress. In order to explore more technologies confronting the high nutrient load in dairy manure, a sequential process combining enhanced solid-liquid separation through flocculation-centrifuge and the subsequent microalgal growth in the liquid part was studied in this study. Solid-liquid separation by flocculation-centrifuge process, using a natural flocculant chitosan and a flocculation aid kaolin, was optimized through response surface methodology (RSM) technique using central composite design (CCD) method.

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Two-phase anaerobic co-digestion of lignocellulosic crop residues with animal wastes can efficiently generate more biogas compared with the digestion of animal waste alone. Non-airtight fermentation of the mixed substrates is the primary step to hydrolyze complex organics and achieve simultaneous phosphorus release. Recycling phosphorus from tremendous animal wastes is remarkably meaningful regarding non-renewable resource recovery.

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Non-airtight fermentation of lignocellulosic agricultural residues with animal wastes is an emerging pretreatment method to produce acid-rich substrates in two-phase anaerobic digestion. Acid-rich hydrolysate could be an excellent feedstock for cultivating microalgae, therefore, the feasibility of a two-step process combining non-airtight fermentation of sugar beet pulp with anaerobically digested dairy manure and mixotrophic microalgae species Chlorella cultivation in the hydrolysate was explored in this study. The hydrolysis and acidification process of 8-day non-airtight fermentation produced up to 8.

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Insufficient denitrification in biological treatment is often a result of the lack of a carbon source. In this study, use of the volatile fatty acids (VFAs) generated via pre-digestion as a carbon source to improve denitrification in sequencing batch reactor (SBR) treatment of liquid swine manure was investigated. The pre-digestion of swine manure was realized by storing the manure in a sealed container in room temperature and samples were taken periodically from the container to determine the VFA levels.

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Grapevine autophagy-related genes (ARGs) include 35 members that have unique evolutionary backgrounds and expression patterns, with some of them responding to abiotic stresses, including copper stress. Autophagy is one of the most crucial self-regulating phenomena in livings organisms, including animals, plants, yeasts, etc. In the genomes of plants, like Arabidopsis, rice, tobacco, and barley, more than 30 autophagy-related genes (ARGs) have been found.

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Grapevine is an important and extensively grown fruit crop, which is severely hampered by drought worldwide. So, comprehending the impact of drought on grapevine genetic resources is necessary. In the present study, RNA-sequencing was executed using cDNA libraries constructed from both drought-stress and control plants.

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This study was undertaken to investigate the effect of two split feeding schemes (600 mL/200 mL and 400 mL/400 mL, designated as FS1 and FS2, respectively) on the performance of a step-fed sequencing batch reactor (SBR) in treating liquid swine manure for nutrient removal. The SBR was run on an 8-h cycle with a repeated pattern of anaerobic/anoxic/aerobic phases in each cycle and the two feedings always occurred at the beginning of each anaerobic phase. A low-level aeration was used (1.

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MicroRNAs (miRNAs), as an important growth regulator, are also involved in gibberellic acid (GA) signaling, revealing much relationship between miRNAs and GA in various plant responses. Grape is highly sensitive to GA, which plays a significant regulatory role in regulation of flower development, berry expansion, berry set, berry ripening, and seedlessness induction; further, it was found that grapevine miR061 (VvmiR061) is a GA responsive miRNA. In this study, grapevine REV (VvREV) and HOX32 (VvHOX32), two target genes of VvmiR061, were predicted, verified, and cloned; homologous conservation was analyzed in various plants.

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There is a lack of literature reporting the methane potential of several livestock manures under the same anaerobic digestion conditions (same inoculum, temperature, time, and size of the digester). To the best of our knowledge, no previous study has reported biochemical methane potential (BMP) predicting models developed and evaluated by solely using at least five different livestock manure tests results. The goal of this study was to evaluate the BMP of five different livestock manures (dairy manure (DM), horse manure (HM), goat manure (GM), chicken manure (CM) and swine manure (SM)) and to predict the BMP using different statistical models.

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Two down-flow wood bark-based biofilters were evaluated for their effectiveness in treating odor, NH3 and H2S under actual swine farm conditions. The water requirement for maintaining proper media moisture contents (MC) under different ventilation rates and intervals were determined. The effect of media depth and MC on the biofilters' performance was also evaluated.

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The aim of this study was to investigate the potential for anaerobic co-digestion of Chinese cabbage waste silage (CCWS) with swine manure (SM). Batch and continuous experiments were carried out under mesophilic anaerobic conditions (36-38°C). The batch test evaluated the effect of CCWS co-digestion with SM (SM: CCWS=100:0; 25:75; 33:67; 0:100, % volatile solids (VS) basis).

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The objective of this study was to investigate the effect of the feed-to-microbe (F/M) ratios on anaerobic digestion of Chinese cabbage waste (CCW) generated from a kimchi factory. The batch test was conducted for 96 days under mesophilic (36.5 °C) (Experiment I) and thermophilic (55 °C) conditions (Experiment II) at F/M ratios of 0.

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The discovery of microRNAs as a novel class of gene expression regulators has led to a new strategy for disease diagnostics and therapeutics. Cell cycle, cell proliferation, and tumorigenesis are all regulated by microRNAs. Several general principles linking microRNAs and cancer have been recently reviewed; therefore, the current review focuses specifically on the perspective of microRNAs in control of cell cycle, stem cells, and heterotypic signaling, as well as the role of these processes in breast cancer.

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A pilot-scale biofilter was developed in which two types of wood chips (western cedar [WC] and 2-in. hardwood [HW]) were examined to treat odor emissions from a deep-pit swine finishing facility in central Iowa. The biofilters were operated continuously for 13 weeks at different airflow rates resulting in variable empty bed residence times (EBRTs) from 1.

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A pilot-scale mobile biofilter was developed where two types of wood chips (western cedar and 2 in. hardwood) were examined to treat odor emissions from a deep-pit swine finishing facility in central Iowa. The biofilters were operated continuously for 13 weeks at different air flow rates resulting in a variable empty bed residence time (EBRT) from 1.

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