The integration of PCR testing in serum into the diagnostic algorithm of suspected acute Q fever in an endemic setting.

Unlabelled: Serum polymerase chain reaction (PCR) for the detection of DNA has been suggested for rapid Q fever diagnosis. We evaluated the role of PCR testing in serum in the diagnosis of acute Q fever in an endemic setting. We examined patients suspected of acute Q fever tested for -specific serum real-time PCR in a tertiary hospital between January 2019 toand December 2022.

NGS in the clinical microbiology settings.

We hypothesized that targeted NGS sequencing might have an advantage over Sanger sequencing, especially in polymicrobial infections. The study included 55 specimens from 51 patients. We compared targeted NGS to Sanger sequencing in clinical samples submitted for Sanger sequencing.