ANTIPROLIFERATIVE ACTIVITIES Of EXTRACTS FROM MYCELIAL BIOMASS OF SOME MEDICINAL BASIDIOMYCETES IN HUMAN COLON CANCER CELLS COLO 205.

Background: The anticancer effects of phytohormones of cytokinin nature are similar to those of medicinal mushrooms, which are able to synthesize cytokinins in large amounts.

Aim: To determine the antiproliferative effect of crude extracts and cytokinin fractions from the mycelial biomass of seven fungi species on colon cancer cells in vitro.

Materials And Methods: Cytokinin content in mycelial biomass of Ganoderma lucidum, Lentinula edodes, Trametes versicolor, Pleurotus ostreatus, Morchella esculenta, Hericium coralloides, and Fomitopsis officinalis was determined by high performance liquid chromatography mass spectrometry.

[Activating effect of a germanium-organic compound on immunocompetent cells during intranasal immunization of mice with a live influenza vaccine].

Aim: Detailed characteristic of results of intranasal immunization of mice with one of two variants of vaccinating influenza virus, particularly in combination with a low molecular weight germanium-organic compound (LMW-GOC). An additional aim is evaluation of effect of LMW-GOC on the parameters of immune system in case of intranasal administration of the preparation without the addition of vaccinating virus.

Materials And Methods: The study was carried out in female CBA mice (18-20 g, 6 animals per group).

[Activation of lymphocytes under the influence of an influenza vaccine combined with a low molecular weight germanium organic compound].

Aim: Confirmation of immunostimulating effect of an original low molecular weight germanium organic compound (LMW-GOC) during immunization of mice with Vaxigrip vaccine.

Materials And Methods: The experiments were carried out in CBA mice divided into 4 groups: control, those that received Vaxigrip influenza vaccine intraperitoneally, those that received LMW-GOC intraperitoneally and those that received both preparations at once. Effect of the preparations administered was evaluated by flow cytofluorometry based on changes of CD3, CD4, CD5, CD8, CD19, CD25, Foxp3, NK1.

[Delayed results of vaccination of children with recurrent respiratory infections against measles and mumps].

Aim: Assessment of results of immunization against measles and mumps in children with recurrent respiratory infections.

Materials And Methods: Levels of IgG against measles and mumps viruses were measured using enzyme immunoassay. Two hundred and twelve serum samples obtained from 6 groups of children with 20 - 45 persons (boys and girls) in each were tested.

[Enhancement of measles-mumps divaccine using myelopeptide 2 in experiment].

Combined application of mumps and measles vaccine strains in equal doses results in significant decrease of immune response to the former component in humans. It is possible that this phenomenon is related with well-known immunodepressive effect of measles virus, which was demonstrated both in vivo and in vitro. It was previously shown that myelopeptide-2 (MP-2) partially neutralizes suppressive effect of measles vaccine on blast transformation of activated human lymphocytes in vitro.

[Gender-specific characteristics of mental capability in students of different specialties].

Mental capability of higher school students of different specialties: technical (27 male and 35 female), natural (32 male and 30 female) and humanitarian (20 male and 26 female) were studied. The following parameters were studied: perception, attention, memory, thinking and mental capability. The results showed that the development of psychophysiological organization of informational processing system in higher school students of different specialties is characterized by geterocronyisms and depend on the field of professional training.

Myelopeptide-2 recovers interleukin-2 synthesis and interleukin-2 receptor expression in human T lymphocytes depressed by tumor products or measles virus.

Myelopeptide-2 (MP-2; Leu-Val-Val-Tyr-Pro-Trp), originally isolated from the supernatant of porcine bone marrow cell culture, is able to restore the mitogen responsiveness of human T lymphocytes inhibited by conditioned medium from HL-60 leukemia cells or measles virus. This effect is based on the ability of MP-2 to recover the reduced interleukin (IL)-2 synthesis and IL-2 receptor (IL-2R) expression in human T lymphocytes treated with these harmful agents. The involvement of other cytokines in MP-2 restoration of the reduced IL-2 synthesis in T lymphocytes is experimentally studied.

[Effect of alimentary cholesterol loading on dynamics of serum testosterone and estradiol in rats ].

Some background mechanisms of the sex hormones changes in blood serum of the male rats under the alimentary loading by cholesterol during 21 weeks were investigated. Results suggested that in the conditions of the cholesterol loading estradiol level in the experimental rats was higher than similar indexes in animals of a control group. In relation to the testosterone level the later was lower during 12 weeks while since 15 week it exceeded the control levels.

[Modulation of electrical and contractile responses of the isolated abdominal aorta in hyperholesterinemia and after treatment with calcium antagonists].

Membrane potential of aorta's endothelium, contractile responses of isolated preparation of the abdominal aorta and their modulation with calcium antagonists were studied at hypercholesterinemia (HHS). Membrane potential has been observed to be slightly depolarized at HHS, and acetylcholine-induced electric and contractile responses were shown to be depressed. Calcium antagonists had positive effect on MP, electric and contractile responses of the preparations of the abdominal aorta of rats with HHS.

[Dynamics of calcium intake in the brain tissue in hypercholesterolemia].

A dynamics of calcium content in rat brain during endogenic hypercholesterolemia, caused by action of the stressful factor (restriction of vital space (80-100 sm2 per one individual) and high salt diet (2 g/kg)) was studied. It was revealed that under the given conditions there is an increase in calcium content in the brain. Nifedipine (2 mg/ kg per day) significantly reduced calcium content in the brain that has allowed making an assumption of some mechanisms of this phenomenon.

[Effect of myelopeptide 2 on the immunization of guinea pigs with live measles vaccine].

A model is suggested to study the effects the myelopeptide 2 (MP2) immunomodulator on the results of live measles rash immunized by vaccine. Guinea pigs were found to respond to live measles vaccine L-16 the way man does, i.e.

[Effect of enalapril on the blood lipid spectrum and on the calcium level in the aorta and liver during stress].

Possible mechanisms of influence of the angiotensinconverting enzyme in the calcigistia dynamics in the aorta and liver and lipid spectrum of the rat blood upon the long-live stress exposure were investigated. It is shown, that the blockade of the angiotensinconverting enzyme leads to the one-type changes of the calcium contents in organs studied, but with different quantity indicators. The contents of general cholesterol and high-density lipoproteins is intimately connected with the calcium contents in liver.

[Relationship between hypercholesteremia and morphological changes in the aorta and kidney].

Effects of exogenic and endogenic hypercholesterinemia on morphological structure of aorta and liver were investigated. Both exogenic and endogenic hypercholesterinemia have been shown to induce different morphological changes in tissues of the aorta and the liver which were more expressed at endogenic hypercholesterinemia, as compared to exogenic one. The data obtained suggest that more pronounced impairments in the tissues examined which led to endogenic hypercholesterinemia were the result of a great number of neuroendocrine mechanisms involved in a stressful reaction.

[Effect of stress on calcium homeostasis in muscular tissues].

A dynamics of the content of calcium (Ca) in aorta, heart and skeletal muscles was studied in rats exposed to a stress factor for 21 weeks. We observed a 7-fold increase in its content in all the organs examined by the middle of an experiment and its progressive decrease by the 21-st week. It might result from the stages of stress and development of hypercholisterinemia.

[Effects of different types of stress on the accumulation of calcium in the liver, and its functional activity].

The dynamics of calcium content in the liver under alimentary and immobilizing stresses was studied. To evaluate the functional state of the liver we conducted a simultaneous recording of the cholesterin level in the serum. Calcium has been shown to be accumulated in that organ, and it correlated positively with the level of cholesteremia.

[Comparative analysis of calcium accumulation in tissues of the brain and the liver in alimentary hypercholesteremia].

Some mechanisms of changing calcium content in brain and liver at alimentary hypercholesteremia were studied. Dynamics of calcium accumulation in tissues of the organs under exploration has proved its contribution to an adaptive reaction of the organism at alimentary stress and reflected peculiarities of its different stages. Similar and simultaneous dynamic changes in calcium accumulation in both organs (liver and brain) seem to be induced by stereotypical pathogenic mechanisms.

[Recurrent inhibition of the flexor muscle motoneurons of the human forearm under the effect of vibration and static muscle strain].

By use of H-reflex technique reflex flexor muscles motoneurons recurrent inhibition was studied for man forearm muscle. It was observed the reduce of this inhibition with the muscles strain. The vibration, acting to the strained muscle, showed a disappearance of inhibition.

Measles IgA in the nasal washings of adult volunteers and children immunized intranasally with measles vaccine L-16.

Earlier it was determined that inspiration of aerosolized measles vaccines may be as effective as its injection in induction of measles antibodies formation. In the beginning of measles infection the measles virus penetrates through mucosa of nose, mouth or eyes of children. So it seems rational to use a nasal spray of measles vaccine to induce "mucosal immunity" in the nasopharinx.

[Intranasal revaccination of children with live measles vaccine: development of local immunity].

Thirty-eight children aged 6-7 years were intranasally revaccinated against measles. Children without antimeasles antibodies in the serum responded to revaccination by their appearance in protective titers. In children with antibodies their titers increased (in 60%) on day 30 after intranasal revaccination.

[Immunodepressive effect of measles and parotitis virus in vitro].

Mechanisms of suppressive effect of measles and parotitis virus on lymphocyte proliferation and cytolytic activity of natural killers are studied in vitro. Both viruses exert a weak mitogenic effect on human blood lymphocytes and at the same time suppress their response to phytohemagglutinin stimulation in vitro. Suppressive effect of measles virus is more expressed than of parotitis virus.

[An intranasal method of revaccination against mumps].

The effectiveness of revaccination against mumps was studied using different routes for administration of a live mumps vaccine from L-3 strain. Poor reactogenicity of the vaccine was observed after intranasal administration. The highest rise in titres of virus-neutralizing antibody was demonstrated in volunteers given the vaccine by the intranasal route.