E-cadherin and β-catenin expression in sebaceous eyelid adenocarcinomas.

Background: Since the E-cadherin-catenin complex is of importance in the cancerogenesis of epithelial tumors, we examined the expression of E-cadherin and β-catenin in sebaceous adenocarcinomas of the eyelid and correlated the results with clinicopathological parameters.

Methods: The study included histological specimen of 32 patients with sebaceous adenocarcinomas of the eyelid. The expression of E-cadherin, β-catenin, and Ki-67 nuclear antigen was analyzed by immunohistochemistry.

[Histopathological study of retrolental membranes secondary to persistent hyperplastic primary vitreous].

Objective: To study the pathological characteristics of retrolental membranes (RLMs) secondary to persistent hyperplastic primary vitreous (PHPV), and to discuss the possible pathogenesis of PHPV.

Methods: Experimental study. Six RLMs obtained from six patients with PHPV during vitrectomy were examined by light microscopy (HE & PAS staining).

Retinoblastoma in the Beijing Tongren Hospital from 1957 to 2006: clinicopathological findings.

Background: To investigate the clinical and histopathological features of eyes enucleated due to retinoblastoma in a 50-year period in China.

Methods: The study included all eyes enucleated for retinoblastoma in the Beijing TongRen Hospital in the study period from 1957 to 2006. The clinical and histological charts, and histological slides were assessed for demographic and clinical data, and histological findings.

Central corneal thickness, lamina cribrosa and peripapillary scleral histomorphometry in non-glaucomatous Chinese eyes.

Background: To examine a potential correlation between a thin cornea, a thin lamina cribrosa and a thin peripapillary sclera in normal eyes of Chinese, as surrogate for a presumed association between a thin cornea and an increased glaucoma susceptibility.

Methods: The histomorphometric study included 55 non-glaucomatous globes (55 patients; mean age: 41.6 ± 13.

Delay in the diagnosis of retinoblastoma in China.

Purpose: Retinoblastoma is the most frequent primary intraocular tumour and is rapidly gaining importance in Asia. As the survival is markedly dependent on early diagnosis and as information on that is not available from East Asia, we assessed the time interval between symptoms detection and therapy of children treated in the Beijing TongRen Eye Hospital.

Methods: The retrospective study consisted of all patients who were treated in the study period from January 1987 to November 2006 in the Beijing TongRen Hospital and for whom the data on delay in diagnosis, age, clinical appearance and histological tumour features were available.

Epithelial tumors of the lacrimal gland in the Chinese: a clinicopathologic study of 298 patients.

Objective: To examine the clinicopathologic characteristics and clinical features of epithelial tumors of lacrimal gland in China.

Methods: The retrospective case series study included all 298 patients of epithelial lacrimal gland tumors which had been collected in the ophthalmic pathologic laboratory of the Tongren Hospital Beijing in the study period from 1961 to 2005.

Results: Pleomorphic adenomas were the most common tumors (n = 213 (72%)), followed by adenoid cystic carcinoma (n = 58 (20%)), pleomorphic adenocarcinoma (n = 13 (4%)), primary adenocarcinoma (n = 8 (3%)) and other tumors (n = 6 (2%)).

Lamina cribrosa and peripapillary sclera histomorphometry in normal and advanced glaucomatous Chinese eyes with various axial length.

Purpose: To measure laminar and peripapillary scleral architecture in normal and glaucomatous Chinese eyes with normal and those with elongated axial length.

Methods: The histomorphometric investigation included a normal group (nonaxially elongated eyes) of 40 human globes (40 patients; mean age, 41.3 +/- 13.

Molecular regulation of vasculogenic mimicry in human uveal melanoma cells: role of helix-loop-helix Id2 (inhibitor of DNA binding 2).

Background: Vasculogenic mimicry (VM) is a tumor angiogenesis process in which highly aggressive melanoma cells form patterned, tubular networks in an in vitro, three-dimensional culture that mimics vasculogenic networks formed by endothelial cells. These cells also express endothelial cell-associated genes such as vascular endothelial-cadherin (VE-cadherin) and are correlated with poor clinical prognosis in patients. However, the molecular underpinnings of this phenomenon remain elusive.

[Clinical and pathological analysis of 2639 cases of eyelid tumors].

Objective: To describe the histopathologic spectrum and patient characteristics of eyelid tumors in Beijing Tongren Hospital.

Methods: It was a claims database analysis. A total of 2639 eyelid tumors verified histopathologically from January 1997 to December 2006 were included in the study.

[Study on the risk factors for optic nerve invasion in 381 cases of retinoblastoma].

Objective: To investigate the clinical and pathologic outcomes of a large group of retinoblastoma (RB) cases to ascertain the risk factors of RB optic nerve invasion.

Methods: In this paper, we summarized the clinical and pathologic data of 381 retinoblastoma cases in Beijing Tongren Hospital observed from 1990 to 2002. Statistical analyses were performed on the sex, age, unilateral or bilateral cases, length of symptoms, clinical situation, stage, histological type and the degree of optic nerve invasion.

[Photodynamic therapy of pigmented choroidal melanomas in rabbits using hematoporphyrin monomethyl ether].

Objective: The purpose is to determine the effectiveness of photodynamic therapy of experimental choroidal melanoma in rabbits using hematoporphyrin monomethyl ether (HMME).

Methods: Pigmented choroidal melanomas were established in 46 New Zealand albino rabbit eyes. The animals were treated with daily injection of cyclosporine A.

Intraocular extension of conjunctival squamous cell carcinoma.

Conjunctival squamous carcinoma, which is regarded as a low-grade malignancy, usually originates at the limbus. Most cases remain superficial to the sclera. Intraocular invasion is rarely reported.

[The effects of matrine on cell proliferation and telomerase activity in retinoblastoma cells in vitro].

Objective: To evaluate the effects of matrine on cell proliferation and telomerase activity in retinoblastoma cells in vitro.

Methods: HXO-Rb44 cells were treated with 0.1 to 3.

[Clinical and pathological features of 112 cases with ocular adnexal lymphoproliferative lesions].

Objective: To investigate the histopathological classification and clinical features of ocular adnexal lymphoproliferative lesions.

Methods: The clinical, histomorphological and immuno-histochemical features of 112 cases of ocular adnexal lymphoproliferative lesions (116 paraffin specimens) were studied retrospectively. The lesions were classified according to the World Health Organization classification of tumors of haematopoietic and lymphoid tissues (2001).

Correlation of the expression of telomerase RNA with risk factors for recurrence of sebaceous gland carcinoma.

Aims: To investigate the expression of human telomerase gene RNA (hTR) and human telomerase reverse transcriptase (hTRT) in eyelid sebaceous gland carcinoma and to evaluate risk factors for sebaceous carcinoma recurrence.

Methods: The expression patterns of hTR and hTRT were detected by in situ hybridization (ISH) in paraffin-embedded samples of 55 eyelid sebaceous gland carcinoma, 12 chalazia, and four sebaceous adenoma. The proliferation index (PI) of sebaceous gland carcinoma was determined by Ki-67 immunolabeling and the results were compared with the expression of hTR and hTRT.

[Clinical significance of mdm2 and p53 expression in orbital rhabdomyosarcoma].

Objective: To evaluate the role of oncogene mdm2 and p53 in the development of orbital rhabdomyosarcoma (ORMS) and demonstrate the feasibility of mdm2 and p53 as biological characteristic markers and prognostic indicators.

Methods: Immunostainings of mdm2, p53 and Ki-67 antigen were performed on archival paraffin-embedded tissues taken from 31 ORMS patients. In situ hybridization was used to demonstrate the expression of mdm2 and p53 on the mRNA level.

[The study on the correlation between telomerase and histopathologic features of retinoblastoma].

Objective: To investigate the correlation between the expression of human telomerase RNA (hTR), human telomerase reverse transcriptase (hTRT) and the proliferation and differentiation of retinoblastoma (RB).

Methods: In 37 RB specimens, the expression of hTR and hTRT was investigated with the methods of in situ hybridization (ISH). Immunohistochemical method was performed to detect the expression of proliferating cell nuclear antigen (PCNA) in RB.

[Clinical and pathological features of 273 cases of lacrimal epithelial tumors].

Objective: A retrospective study of histopathological and clinical aspects of 261 cases of lacrimal tumors (273 paraffin specimens) from Ophthalmic Pathology Laboratory, Beijing Tongren Hospital (Dec. 1961 to Jun. 2002) was performed to investigate the pathological classification and clinical features of these tumors.

Telomerase expression in sebaceous carcinoma of the eyelid.

Background: In humans telomerase is expressed in most cancers and immortal cell lines, and activation of telomerase may play important roles in tumorigenesis and immortalization. This study was to investigate the roles of telomerase activity (TA) and human telomerase RNA (hTR) in sebaceous carcinoma of the eyelid.

Methods: The telomerase repeated amplification protocol (TRAP) was used to demonstrate telomerase activity in 12 cases of sebaceous carcinoma of the eyelid.

[Correlation of tumor angiogenesis with clinicopathologic prognostic parameters in choroidal melanoma].

Objective: Tumor angiogenesis is an essential step for tumor growth, progression and metastasis. Vascular endothelial growth factor (VEGF) is a mitogen specific for vascular endothelial cells, and plays a key role in tumor angiogenesis. The purpose of the present study was to investigate the expression of VEGF mRNA and VEGF protein in choroidal melanoma and to correlate these factors and the microvessel density (MVD) with the clinicopathologic prognostic features.

[Telomerase expression in tarsal gland carcinoma].

Objective: To investigate the roles of telomerase activity (TA) and human telomerase RNA (hTR) in tarsal gland carcinoma.

Methods: The telomerase repeated amplification protocol (TRAP) was used to demonstrate telomerase activity in 12 tarsal gland carcinomas. In situ hybridization (ISH) was used to demonstrate the expression of hTR in 55 cases of paraffin-embedded tarsal gland carcinoma, and the results were compared with the proliferative index determined by Mib-1 immunolabeling, histological patterns and recurrence of the tumor.