affects myocardial function through TGF-β/Smad axis and pirfenidone.

Cardiac arrhythmias are often linked to the overactivity of cardiac fibroblasts (CFs). Investigating the impact of poly (ADP-ribose) polymerase 9 (PARP9) on Angiotensin II (Ang II)-induced fibroblast activation and the therapeutic effects of pirfenidone (PFD) offers valuable insights into cardiac arrhythmias. This study utilized weighted gene co-expression network analysis (WGCNA), differential gene expression (DEG) analysis, protein-protein interaction (PPI), and receiver operating characteristic (ROC) analysis on the GSE42955 dataset to identify the hub gene with a significant diagnostic value.

Dynamic characteristics of coal gangue subgrade filler under traffic load based on the hysteretic curves.

Traffic cyclic loading is the key factor that leads to the deterioration of the long-term service behavior of subgrade. A series of cyclic triaxial tests was carried out by the large-scale dynamic and static triaxial apparatus (LSDSTA) to study the dynamic behaviors of coal gangue subgrade filler (CGSF) under multi-step cyclic loading using the morphological characteristics of hysteretic curves (MCHC). MCHC was quantitatively characterized by four parameters, i.

Reverse transcription recombinase-aided amplification assay combined with a lateral flow dipstick for detection of duck Tembusu virus.

Duck Tembusu virus disease, caused by duck Tembusu virus (DTMUV), brings great harm to duck industry. Early diagnosis is of great significance for the prevention and control of this disease. In order to develop a specific and sensitive method for rapid diagnosis of DTMUV, reverse-transcriptase recombinase aided amplification combined with lateral flow dipstick (RT-RAA-LFD) method for detection of DTMUV was established.

A novel reverse transcription recombinase polymerase amplification assay for rapid detection of GI.1 genotype of rabbit hemorrhagic disease virus.

Rabbit Viral Hemorrhagic Disease (RHD) is a highly contagious and fatal infection, resulting in considerable economic losses to the rabbit industry. Consequently, it is essential to develop a fast and accurate diagnostic method for RHDV GI.1.

Application of L-moment method for regional frequency analysis of meteorological drought across the Loess Plateau, China.

Water shortages have always been the primary bottleneck for the healthy and sustainable development of the ecological environment on the Loess Plateau (LP). Proper water resource management requires knowledge of the spatiotemporal characteristics of precipitation frequency. This paper employed the gridded precipitation dataset obtained from the China Meteorological Data Service Centre to present a spatially explicit characterization of precipitation frequencies in tandem with their return periods on the LP based on the L-moment method.

Rapid Degradation of SARS-CoV-2 Spike S Protein by A Specific Serine Protease.

The S protein of SARS-CoV-2 is a crucial structural and functional component for virus entry. Due to the constant mutation of the virus, there are very limited ways to prevent and control COVID-19. This experiment used a macroscopic SDS-PAGE method and proved that the S protein of wild-type SARS-CoV-2 virus, especially the S1 subunit, is very sensitive to alkaline serine protease with acidic pI (ASPNJ), NJ represents (Izuka) from which ASP is purified).

Protective Effect of Znt7 on High Glucose-Induced Epithelial-to-Mesenchymal Transition in Renal Tubular Epithelial Cells.

Background/aims: Evidence from our and other groups has demonstrated that zinc transporter 7 in SLC30 family (ZnT7) inhibited epithelial-to-mesenchymal transition (EMT) and apoptosis in rat peritoneal mesothelial cells (RPMCs) under high glucose (HG) concentration. In the present study, we investigated the effect of ZnT7 on EMT of renal tubular epithelial cells (RTECs) in an in vitro model of diabetic nephropathy (DN).

Methods: A dual-fluorescent staining protocol was used for detection of ZnT7 in a normal rat kidney tubular epithelial cell line (NRK-52E cells).

Getting a Grip on NCDs in China: an Evaluation of the Implementation of the Dutch-China Cardiovascular Prevention Program.

Purpose: China has experienced a rapid increase in cardiovascular diseases and related chronic conditions over the last 20 years, and there is now an urgent need for new approaches that can effectively reduce the progression of cardiovascular disease in high-risk individuals. This article reports on the evaluation of the implementation of the Dutch-China Cardiovascular Prevention Program.

Methods: A screening questionnaire, based on the Dutch concept of Prevention-Consultation, was distributed among inhabitants in seven communities in Anhui Province, China.

A novel alkaline serine protease with fibrinolytic activity from the polychaete, Neanthes japonica.

A new protease named NJP with fibrinolytic activity was isolated from Neanthes japonica (Izuka), by a combination of ammonium sulfate fractionation, hydrophobic chromatography, ion-exchange chromatography and gel filtration. The molecular mass of NJP was approximately 28.6-33.

Neanthes japonica (Iznka) fibrinolytic enzyme reduced cerebral infarction, cerebral edema and increased antioxidation in rat models of focal cerebral ischemia.

Thrombolytic agent is increasingly being used in treating acute ischemic stroke. A novel protease with strong thrombolytic activity, Neanthes japonica (Iznka) fibrinolytic enzyme (NJF) discovered in our laboratory has been reported with characteristics of direct hydrolyzing fibrin and fibrinogen. The neuroprotective effect of NJF and urokinase (UK) was tested in rat models of middle cerebral artery occlusion (MCAO).

Purification and characterization of a novel fibrinolytic enzyme from the polychaete, Neanthes japonica (Iznka).

A novel fibrinolytic enzyme from Neanthes japonica (Iznka), named NJF, was purified to electrophoretic homogeneity using ammonium sulfate precipitation, hydrophobic interaction, ion exchange and gel-filtration chromatography. NJF consisted of a single polypeptide chain with a molecular weight of 28-32 kDa, which was determined by MALDI-TOF mass spectrum and SDS-PAGE. The isoelectric point of NJF determined by isoelectric focusing electrophoresis (IEF) was 4.