High-temperature pressure cooking on the quality of Basa (Pangasius bocourti) fish maw: Gelling, in vitro digestion and proteomic analysis.

This study aims to evaluate the different processing methods, soaking-high-temperature pressure cooking (SHPC) and un-soaking-high-temperature pressure cooking (USHPC), on the quality of Basa (Pangasius bocourti) fish maw (BFM). Compared to traditionally cooked BFM, SHPC and USHPC treated BFM exhibited more severe disruption of collagen fibrils and network structure, significantly decreased texture, shear force and water holding capacity of BFM with the increase of HPC time, indicating that HPC could effectively reduce soaking time of BFM. Besides, as time HPC was 30 min, USHPC almost showed similar texture results to SHPC, and the former had the higher in vitro digestibility.

A cytological revisit on parthenogenetic Artemia and the deficiency of a meiosis-specific recombinase DMC1 in the possible transition from bisexuality to parthenogenesis.

Although parthenogenesis is widespread in nature and known to have close relationships with bisexuality, the transitional mechanism is poorly understood. Artemia is an ideal model to address this issue because bisexuality and "contagious" obligate parthenogenesis independently exist in its congeneric members. In the present study, we first performed chromosome spreading and immunofluorescence to compare meiotic processes of Artemia adopting two distinct reproductive ways.

Downregulation of a CT10 regulator of kinase (Crk) promotes the formation of diapause embryos in the brine shrimp Artemia.

To survive under harsh environments, embryonic development of Artemia was arrested at the gastrula stage and released as the diapause embryo. Cell cycle and metabolism were highly suppressed in this state of quiescence. However, cellular mechanisms underlying diapause remain largely unclear.

Identification and characterization of the Doublesex gene and its mRNA isoforms in the brine shrimp Artemia franciscana.

Doublesex (DSX) proteins are members of the Doublesex/mab-3-related (DMRT) protein family and play crucial roles in sex determination and differentiation among the animal kingdom. In the present study, we identified two Doublesex (Dsx)-like mRNA isoforms in the brine shrimp Artemia franciscana (Kellogg 1906), which are generated by the combination of alternative promoters, alternative splicing and alternative polyadenylation. The two transcripts exhibited sex-biased enrichment, which we termed AfrDsxM and AfrDsxF.

Psychosocial correlates of fertility-related quality of life among infertile women with repeated implantation failure: The mediating role of resilience.

Objective: This study aimed to examine associations between psychosocial factors and fertility-related quality of life (FertiQoL) among infertile women with repeated implantation failure (RIF), and to identify the possible role of resilience in mediating the effect of social support on FertiQoL.

Materials And Methods: A cross-sectional study was conducted with 234 infertile women with RIF in total. Fertility quality of life scale (FertiQoL), perceived social support scale (PSSS), and Connor-Davidson Resilience Scale (CD-RISC) were used to evaluate the patients.

[Study on physicochemical properties and influence factors on stability of breviscapine].

Objective: To investigate the physicochemical constants such as equilibrium solubility and apparent partition coefficients (Papp), and study the effects of temperature, pH value and antioxidants on the stability of breviscapine solution.

Method: The equilibrium solubility of breviscapine in various medium and the Papp of breviscapine under different pH conditions were determinated by RP-HPLC, and the effects of temperature, pH value and antioxidants on the stability of solution were investigated by taking change rates of drug content in 10 h as detection index.

Result: The equilibrium solubility of breviscapine in saline, distilled water, pH 7.

[Comparison of the distribution of breviscapine in the brain by different administration routes].

By comparing the drug distribution of breviscapine administered intranasally, orally and intrgvenous injected in rats' brain. After 0.4 mg x kg(-1) breviscapine was given by tail vein, intranasal and gastric perfusion administration to SD rats, cerebrospinal fluid was obtained by erebellomedllery cisternal puncture at different times.

[Evaluation of targeting property for Kuikang colon targeted pellets].

Objective: To evaluate colon targeting characteristic of Kuikang colon targeted pellets (KCP) with determination of residual baicalin and baicalein concentration in gastrointestinal tract (GIT).

Method: The baicalin and baicalein were assayed by HPLC. The recovery differences of the drug between KCP and conventional pellets from GIT were investigated, three and six hours after administration.

[Preliminary study on sea cucumber saponin-nobiliside A liposome and its hemolytic activity].

Nobiliside A (Nob A) liposomes were prepared. Its assay method of content and encapsulation efficiency (EE) were established, and hemolytic activity with Nob A solution in vivo and in vitro were compared. Preparative method, phospholipid content, ratio of phospholipid to cholesterol and ratio of drug to lipids were optimized by single factor exploration.

[Pharmacokinetics of pueraria for intranasal on spray in rabbits].

Objective: To explore pharmacokinetic features of puerarin in pueraria spray and calculate pharmacokinetic parameters according to puerarin of drug-time curve in rabbits.

Methods: The concentration of puerarin in plamsa was determined by HPLC. The methanol was used to sediment protine.

[Study on purification of Radix Paeoniae Alba by HPD100 macroporous resin].

Objective: To set up the method for purification of paeoniflorin by HPD100 macroporous resin.

Methods: With the adsorption ration and desorption rate of paeoniflorin as the evaluating quotas, the single factor test, orhogonal experimental designs and variance analysis were applied to optimize the manipulation parameters of macrporous resin.

Results: The HPD100 had the best adsorbive and separative properties to paeoniflorin.

[Study on the permeability of 125I-sinomenine via the transdermal delivery way in rats].

Objective: To study the permeance and diffusion trend of 125I-sinomenine via the transdermal delivery way in rats.

Methods: Assay the permeance depth and diffusion area of sinomenine labeled by isotopic 125I in subcutaneous of rats certain time after sinomenine was administrated on the cervical skin of rats.

Results: The diffuse of sionmenine can reach cervical spine in longitudinal direction and form an area of 2 cm2 in transverse direction.