[Detection of metabolites of tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in lung cancer smokers' urine].

Background: It was reported that tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) was a powerful pulmonary carcinogen, predominantly inducing adenocarcinoma of the lung in mouse. The aim of this study is to assay metabolites of NNK, which are 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) and its O-glucuronide (NNAL-Gluc), and their ratio (NNAL-Gluc/NNAL) in smokers and non-smokers' urine, and to explore the carcinogenicity of NNK among different people.

Methods: Using high pressure liquid chromatograph (HPLC) and gas chromatograph-mass tadom (GC-MS/MS), NNAL-Gluc and NNAL in 24h urine were detected in 8 healthy smokers, 10 lung cancer smokers and 4 healthy non-smokers.

[Susceptibility test to rifampin by using quantitative analysis of Mycobacterium tuberculosis mRNA].

Objective: To evaluate the significance of quantitative analysis of Mycobacterium tuberculosis mRNA in the test of susceptibility of Mycobacterium tuberculosis strains to rifampin.

Methods: The susceptibility to rifampin of fifty-three clinical isolated strains was test by the percentage of Mycobacterium tuberculosis 85B mRNA copies before and after the use of rifampin, and 1% and 10% were used as the standards for drug resistance, which was compared with the absolute concentration method. Among them, 29 were rifampin resistant strains and 24 rifampin sensitive strains.

[Quantitative analysis of mRNA as a marker for viability of Mycobacterium tuberculosis].

Objective: To study whether quantitative analysis of M. tuberculosis mRNA could be used to assess bacterial viability.

Methods: The levels of M.

[Pharmacodynamics and pharmacokinetics of domestic fixed-dose combination of antituberculosis drugs].

OBJECTIVE To study the pharmacodynamics and pharmacokinetics of domestic fixed-dose of antituberculosis drugs and to evaluate its quality and activity against Mycobacterium tuberculosis both in vitro and in vivo. METHODS The MIC was determined by the tube doubling dilution method, and the effect of the drugs was assessed by half survival time of the mice. A single oral dose of domestic and imported fixed-dose combination of antituberculosis drugs was given to healthy volunteers, and the drug concentration in serum was determined by HPLC.