Integrative single-cell and spatial transcriptome analysis reveals heterogeneity of human liver progenitor cells.

Background: Liver progenitor cells (LPCs) with bipotential differentiation capacities are essential for restoring liver homeostasis and hepatocyte population after damage. However, the low proportion and shared markers with epithelial cells make studying LPC heterogeneity difficult, especially in humans. To address this gap, we explored over 259,400 human liver single cells across 4 conditions (fetal, healthy, cirrhotic, and HCC-affected livers).

Research on Precision Medicine AI Algorithm for Neuro Immune Gastrointestinal Diseases based on Quantum Biochemistry and Computational Cancer Genetics.

Objective: The objective of this study is to conduct network toxicology analysis based on smoking habits and develop a simpler and more effective toxicology product ingestion control system.

Background: Smoking behavior can affect the pathogenesis and prognosis of neuroimmune gastrointestinal diseases.

Aims: The purpose of developing tools to assist clinical practice is to avoid the harm of cigarettes to the human body.

Super-Concentrated Alkali Hydroxide Electrolytes for Rechargeable Zn Batteries.

Increasing the salt concentration of an electrolyte to over 10 M brings new solute-solvent interactions that define an emerging class of super-concentrated electrolytes for rechargeable batteries. To this class we introduce a super-concentrated alkaline electrolyte. Nearly saturated with KOH (at 15 M), the aqueous solution displays a broad electrochemical stability window (>2.

Research on Neuroimmune Gastrointestinal Diseases Based on Artificial Intelligence: Molecular Dynamics Analysis of Caffeine and DRD3 Protein.

Objectives: The aim of this study was to develop a clinical application model for the rational use of caffeine.

Background: Caffeine is related to the incidence of neuro immune gastrointestinal diseases. Coffee consumption needs to be optimized in order to reduce the incidence rate.

Comparison of the epidemiological characteristics of mycoplasma pneumoniae infections among children during two epidemics in Wuhan from 2018 to 2024.

Background: Mycoplasma pneumoniae (M pneumoniae, MP) is a common pathogen causing respiratory tract infections, particularly in children. In 2023, a resurgence of MP epidemics was observed in Wuhan, Hubei Province, China. This study aims to examine the epidemiological trends and clinical characteristics of MP infections among children in Wuhan from 2018 to 2024, providing valuable scientific evidence to guide local prevention strategies.

Design of Fluorinated Peptides as Biotransformed Urinalysis Biomarkers for Non-Invasive Diagnosis and Treatment of Liver Injury through Enzyme Directed Kinetics.

Urinalysis, as a non-invasive and efficient diagnostic method, is very important but faces great challenges due to the complex compositions of urine and limited naturally occurring biomarkers for diseases. Herein, by leveraging the intrinsic absence of endogenous fluorinated interference, a strategy with the enzymatically activated assembly of synthetic fluorinated peptide for cholestatic liver injury (CLI) diagnosis and treatment through F nuclear magnetic resonance (NMR) urinalysis and efficient drug retention is developed. Specifically, alkaline phosphatase (ALP), overexpressed in the liver of CLI mice, triggers the assembly of fluorinated peptide, thus, directing the traffic and dynamic distribution of the synthetic biomarkers after administration, whereas CLI mice display much slower clearance of peptides through urine as compared with healthy counterparts.

Single-cell analysis of human peripheral blood reveals high immune response activity in successful ageing individuals.

Beneficial remodeling of the immune system in successful ageing individuals (centenarians and supercentenarians) is critical for healthy ageing. However, mechanisms for dynamic regulation of immunity during ageing remain unclear. We use single-cell RNA sequencing (scRNA-seq) as an analytical strategy to study the dynamic regulation of immunity during aging and its molecular mechanisms at the single-cell level.

Wideband optically transparent absorber based on multilayer ITO films with high absorption rate.

In this paper, a wideband optically transparent absorber based on multilayer indium tin oxide (ITO) films is designed, fabricated, and measured. The structure uses polyethylene terephthalate (PET) and ITO films to replace the dielectric substrate and metal pattern of the traditional absorber to achieve optical transparency characteristics. The absorption of the proposed absorber is above 90% from 4.

Pollutant emission during pyrolysis of waste wind turbine blades: Nitrogen-containing components and polycyclic aromatic hydrocarbons.

Serious attention was lacked for various pollutants formed in both gas and tar phase during pyrolysis recycling of waste wind turbine blades (WWTB), especially for components of carcinogenic bisphenol A (BPA) and potentially toxic polycyclic aromatic hydrocarbons (PAHs) in tar. Pyrolysis temperature within 400-600 °C would significantly impact pollutant formations. Additionally, CO had a potential to mitigate pollutants emission as an economic alternative for N.

Optimized Driving Scheme for Three-Color Electrophoretic Displays Based on the Elimination of Red Ghost Images.

Three-color electrophoretic display (EPD) is emerging as a display technology due to its extremely low energy consumption and excellent reflective properties. However, in the process of black and white color image transition, due to the different driving characteristics of red particles, the particles within the three-color EPD cannot be ideally driven to the target position, resulting in the appearance of a red ghost image. For this reason, this study utilized the COMSOL 5.

Valence-engineered catalysis-selectivity regulation of molybdenum oxide nanozyme for acute kidney injury therapy and post-cure assessment.

The optimization of the enzyme-like catalytic selectivity of nanozymes for specific reactive oxygen species (ROS)-related applications is significant, and meanwhile the real-time monitoring of ROS is really crucial for tracking the therapeutic process. Herein, we present a mild oxidation valence-engineering strategy to modulate the valence states of Mo in Pluronic F127-coated MoO nanozymes (denoted as MF-x, x: oxidation time) in a controlled manner aiming to improve their specificity of HO-associated catalytic reactions for specific therapy and monitoring of ROS-related diseases. Experimentally, MF-0 (Mo average valence 4.

Synthesis of Pt-MoS with enhanced photothermal and peroxidase-like properties and its antibacterial application.

Despite tremendous efforts, bacterial infection and contamination remain a major clinical challenge to modern humans. Nanozyme materials with stimuli-responsive properties are expected to be powerful tools for the next generation of antibacterial therapy. Here, MoS nanosheet was firstly prepared by liquid phase exfoliation method, and Pt-MoS hybrid biomaterial was then successfully synthesized by a simple self-reduction method.

Multiexcitation Peaks and Multicolor Emission Nanoassemblies for Transmembrane Cell Imaging and Photoresponsivity Antibacterial.

Nanomaterials with photoresponsivity have garnered attention due to their fluorescence imaging, photodynamic, and photothermal therapeutic properties. In this study, a photoresponsivity nanoassembly was developed by using photosensitizers and carbon dots (CDs). Due to their multiple excitation peaks and multicolor fluorescence emission, especially their membrane-permeating properties, these nanoassemblies can label cells with multiple colors and track cell imaging in real time.

scZAG: Integrating ZINB-Based Autoencoder with Adaptive Data Augmentation Graph Contrastive Learning for scRNA-seq Clustering.

Single-cell RNA sequencing (scRNA-seq) is widely used to interpret cellular states, detect cell subpopulations, and study disease mechanisms. In scRNA-seq data analysis, cell clustering is a key step that can identify cell types. However, scRNA-seq data are characterized by high dimensionality and significant sparsity, presenting considerable challenges for clustering.

Dual role of dissolved black carbon in sensitized ofloxacin photooxidation: Mechanism and influential factors.

Dissolved black carbon (DBC) is the more photoactive component of dissolved organic matter (DOM) pool, which plays a dual role in the photoconversion of aquatic contaminants, acting as both a photosensitizer and an inhibitor. However, little is known about the more systematic mechanism by which DBC exhibits a dual effect, which is closely related to the structure composition of DBC. In this study, the differences in characteristics of DBC obtained from 300 °C and 500 °C were compared via UV-vis absorption spectrum, Fluorescence excitation emission matrix spectra (3D-EEM), Fourier transform infrared (FT-IR), and X-ray photoelectron spectroscopy (XPS), and evaluated the promoting and inhibiting effects of DBC on ofloxacin (OFL) photodegradation.

Incretin-based drugs decrease the incidence of prostate cancer in type 2 diabetics: A pooling-up analysis.

Incretin-based drugs, a class of Antidiabetic medications (ADMs) used in the treatment of type 2 diabetes, may affect the incidence of prostate cancer (PCa). But real-world evidence for this possible effect is lacking. Therefore, the aim of this study is to assess the effect of incretin-based drugs on the incidence of PCa, including glucagon-like peptide-1 (GLP-1) receptor agonists and dipeptidyl peptidase-4 (DPP-4) inhibitors.

CPPLS-MLP: a method for constructing cell-cell communication networks and identifying related highly variable genes based on single-cell sequencing and spatial transcriptomics data.

In the growth and development of multicellular organisms, the immune processes of the immune system and the maintenance of the organism's internal environment, cell communication plays a crucial role. It exerts a significant influence on regulating internal cellular states such as gene expression and cell functionality. Currently, the mainstream methods for studying intercellular communication are focused on exploring the ligand-receptor-transcription factor and ligand-receptor-subunit scales.

Dietary exposure to 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) induces oxidative damage promoting cell apoptosis primarily via mitochondrial pathway in the hepatopancreas of carp, Cyprinus carpio.

To investigate the mechanisms of BDE-47 on hepatotoxicity in fish, this study examined the effects of dietary exposure to BDE-47 (40 and 4000 ng/g) on carp for 42 days. The results showed that BDE-47 significantly increased carp's condition factor and hepatosomatic index. Pathological results revealed unclear hepatic cord structure, hepatocytes swelling, cellular vacuolization, and inflammatory cell infiltration in the hepatopancreas of carp.

Enhancing lung cancer detection through hybrid features and machine learning hyperparameters optimization techniques.

Machine learning offers significant potential for lung cancer detection, enabling early diagnosis and potentially improving patient outcomes. Feature extraction remains a crucial challenge in this domain. Combining the most relevant features can further enhance detection accuracy.

Salvia miltiorrhiza polysaccharide promotes the health of crayfish (Procambarus clarkii) by promoting hemocyte phagocytosis, protecting hepatopancreas and enhancing intestinal barrier function.

Plant polysaccharides as immunomodulators are considered one of the effective measures to reduce antibiotic therapy in aquaculture. The immunomodulatory function of Salvia miltiorrhiza polysaccharides (SMP) has been demonstrated and begun to be applied in vertebrates, but its potential effect on crustaceans is unclear. In this study, crayfish (Procambarus clarkii) was fed with 0 %, 0.