Discovering the Bioactive Compounds Binding to α-Adrenergic Receptor in Guizhi Fuling Formula and Revealing Their Interactions by Immobilizing the Receptor Through Colicin L7 DNase/Immunity Protein 7 Ultra-Affinity System.

In this work, Guizhi Fuling Formula (GFF), as well as α-adrenergic receptor (α-AR) were taken as the research objects. By utilizing the ultra-affinity between Colicin L7 DNase (CL7) and its homologous immune protein 7 (Im7), CL7-tagged α-AR was oriented immobilized to the Im7-coated silica gel surface. With the α-AR immobilized column in hand, the active compounds in GFF targeted to α-AR were screened, and the binding procedures were analyzed.

[Establishment and evaluation of haloalkane dehalogenase tagged -adrenergic receptor chromatography].

Receptor chromatography is an efficient analytical technique that combines the high separation ability of chromatography with the high specificity of receptors for drug recognition. In addition, this technique offers the advantages of active recognition, online separation, and convenient multidimensional target tracking. This strategy allows target active ingredients in complex systems, such as traditional Chinese medicines, to be efficiently screened and accurately identified.

Introgression of tetraploid 6EL segments enhances the stripe rust resistance of adult wheat plants.

Unlabelled: Preventing the widespread occurrence of stripe rust in wheat largely depends on the identification of new stripe rust resistance genes and the breeding of cultivars with durable resistance. In previous study, we reported 6E of wheat-tetraploid 6E (6D) substitution line contains adult-stage stripe rust resistance genes. In this study, three novel wheat-tetraploid translocation lines were generated from the offspring of a cross between common wheat and the 6E (6D) substitution line.

Screening and analysis of the targeted compounds in Choerospondias axillaris extract by receptor chromatographic column with immobilized angiotensin II type 1 receptor.

As a result of the lack of modern techniques, the study of Tibetan medicine has been hindered in identifying bioactive compounds. Herein, we established a chromatographic approach using an immobilized angiotensin II type 1 receptor (ATR) via a one-step method triggered by haloalkane dehalogenase. The bioactive compounds from Choerospondias axillaris (Guangzao) were screened and identified using the immobilized ATR followed by MS.

RNA-seq analysis revealed considerable genetic diversity and enabled the development of specific KASP markers for .

, which grows exclusively in Huashan, China, is an important wild relative of common wheat that has many desirable traits relevant for wheat breeding. However, the poorly characterized interspecific phylogeny and genomic variations and the relative lack of species-specific molecular markers have limited the utility of as a genetic resource for enhancing wheat germplasm. In this study, we sequenced the transcriptome, resulting in 50,337,570 clean reads that were assembled into 65,617 unigenes, of which 38,428 (58.