Integrative multiomic analysis identifies distinct molecular subtypes of NAFLD in a Chinese population.

Nonalcoholic fatty liver disease (NAFLD) has become a common health care burden worldwide. The high heterogeneity of NAFLD remains elusive and impairs outcomes of clinical diagnosis and pharmacotherapy. Several NAFLD classifications have been proposed on the basis of clinical, genetic, alcoholic, or serum metabolic analyses.

Clinical significance of T helper cell subsets in the peripheral blood and bone marrow of patients with multiple myeloma.

Background: T helper (Th) cell subsets primarily assist B cells in differentiating into plasma cells in the germinal center. The mechanism of malignant transformation of plasma cells is an important target for the clinical treatment of MM; however, the mechanism remains unclear.

Methods: We collected the peripheral blood (PB) and bone marrow (BM) samples of 33 patients with MM.

Hepatic GDP-fucose transporter SLC35C1 attenuates cholestatic liver injury and inflammation by inducing CEACAM1 N153 fucosylation.

Background And Aims: Inflammatory response is crucial for bile acid (BA)-induced cholestatic liver injury, but molecular mechanisms remain to be elucidated. Solute Carrier Family 35 Member C1 (SLC35C1) can transport Guanosine diphosphate-fucose into the Golgi to facilitate protein glycosylation. Its mutation leads to the deficiency of leukocyte adhesion and enhances inflammation in humans.

AGDF-Net: Learning Domain Generalizable Depth Features With Adaptive Guidance Fusion.

Cross-domain generalizable depth estimation aims to estimate the depth of target domains (i.e., real-world) using models trained on the source domains (i.

Evaluation of Self-Degradation and Plugging Performance of Temperature-Controlled Degradable Polymer Temporary Plugging Agent.

Temporary plugging diversion fracturing (TPDF) technology has been widely used in various oil fields for repeated reconstruction of high-water-cut old oil wells and horizontal well reservoir reconstruction. Previous studies have carried out in-depth study on the pressure-bearing law and placement morphology of different types of temporary plugging agents (TPAs) in fractures, but there are relatively few studies on TPA accumulation body permeability. To solve this problem, an experimental device for evaluating the TPA performance with adjustable fracture pores is proposed in this paper.

Digging Into Uncertainty-Based Pseudo-Label for Robust Stereo Matching.

Due to the domain differences and unbalanced disparity distribution across multiple datasets, current stereo matching approaches are commonly limited to a specific dataset and generalize poorly to others. Such domain shift issue is usually addressed by substantial adaptation on costly target-domain ground-truth data, which cannot be easily obtained in practical settings. In this paper, we propose to dig into uncertainty estimation for robust stereo matching.

Organic Anion Transporting Polypeptide (OATP) 1B3 is a Significant Transporter for Hepatic Uptake of Conjugated Bile Acids in Humans.

Background & Aims: OATP1B3/SLCO1B3 is a human liver-specific transporter for the clearance of endogenous compounds (eg, bile acid [BA]) and xenobiotics. The functional role of OATP1B3 in humans has not been characterized, as SLCO1B3 is poorly conserved among species without mouse orthologs.

Methods: Slc10a1-knockout (Slc10a1), Slc10a1 (endogenous mouse Slc10a1 promoter-driven human-SLCO1B3 expression in Slc10a1 mice), and human SLCO1B3 liver-specific transgenic (hSLCO1B3-LTG) mice were generated and challenged with 0.

TUSR-Net: Triple Unfolding Single Image Dehazing with Self-Regularization and Dual Feature to Pixel Attention.

Single image dehazing is a challenging and illposed problem due to severe information degeneration of images captured in hazy conditions. Remarkable progresses have been achieved by deep-learning based image dehazing methods, where residual learning is commonly used to separate the hazy image into clear and haze components. However, the nature of low similarity between haze and clear components is commonly neglected, while the lack of constraint of contrastive peculiarity between the two components always restricts the performance of these approaches.

Novel urinary protein panels for the non-invasive diagnosis of non-alcoholic fatty liver disease and fibrosis stages.

Background & Aims: There is an unmet clinical need for non-invasive tests to diagnose non-alcoholic fatty liver disease (NAFLD) and individual fibrosis stages. We aimed to test whether urine protein panels could be used to identify NAFLD, NAFLD with fibrosis (stage F ≥ 1) and NAFLD with significant fibrosis (stage F ≥ 2).

Methods: We collected urine samples from 100 patients with biopsy-confirmed NAFLD and 40 healthy volunteers, and proteomics and bioinformatics analyses were performed in this derivation cohort.

Gut Microbiota Deficiency Exacerbates Liver Injury in Bile Duct Ligated Mice via Inflammation and Lipid Metabolism.

Bile components play a critical role in maintaining gut microbiota homeostasis. In cholestasis, bile secretion is impaired, leading to liver injury. However, it remains to be elucidated whether gut microbiota plays a role in cholestatic liver injury.

Semaphorin 7A interacts with nuclear factor NF-kappa-B p105 via integrin β1 and mediates inflammation.

Semaphorin7a (SEMA7A), a membrane-anchored member of the semaphorin protein family, could be involved in a diverse range of immune responses via its receptor integrin β1. Recently, we reported that the SEMA7A mutation (a gain-of-function mutation, Sema7a in mice) is a risk factor for progressive familial intrahepatic cholestasis and nonalcoholic fatty liver disease via upregulated membrane localization. In this study, we demonstrated that integrin β1 is a membrane receptor for nuclear factor NF-kappa-B p105 (NF-κB p105) and a critical mediator of inflammation.

Hepatic TNFRSF12A promotes bile acid-induced hepatocyte pyroptosis through NFκB/Caspase-1/GSDMD signaling in cholestasis.

Tumor necrosis factor receptor superfamily member-12A (TNFRSF12A) plays a critical role in inflammation and cell death. It is expressed in multiple tissues yet extremely low in normal liver. To date, little is known about its role in cholestasis.

Runt-related transcription factor-1 ameliorates bile acid-induced hepatic inflammation in cholestasis through JAK/STAT3 signaling.

Background And Aims: Bile acids trigger a hepatic inflammatory response, causing cholestatic liver injury. Runt-related transcription factor-1 (RUNX1), primarily known as a master modulator in hematopoiesis, plays a pivotal role in mediating inflammatory responses. However, RUNX1 in hepatocytes is poorly characterized, and its role in cholestasis is unclear.

Hepatic Aquaporin 10 Expression Is Downregulated by Activated NFκB Signaling in Human Obstructive Cholestasis.

Background and Aims: Recent studies reported that the hepatic expression of AQP8 and AQP9 was downregulated in bile duct-ligated (BDL) rats and that overexpression of human AQP1 in the rat liver attenuated cholestasis. However, the hepatic expression of AQP10 and its regulatory mechanism in human cholestasis remain unclear.

Methods: Serum and liver samples were collected from 34 patients with obstructive cholestasis and from 12 control patients.

Berberine Inhibits Herpes Simplex Virus 1 Replication in HEK293T Cells.

Berberine exhibits polytrophic medicinal roles in various diseases and is safe and effective. However, its role and the underlying mechanism in the replication of herpes simplex virus 1 (HSV-1) remain unreported. This research aimed to determine the functional mechanisms of berberine on HSV-1 infection.

Establishing Reference Values for Peripheral Blood Lymphocyte Subsets of Healthy Children in China Using a Single Platform.

Lymphocyte subsets significantly change during childhood; thus, age-matched reference values derived from healthy children are crucial. We established reference values for lymphocyte subsets, including T cells (CD3+), CD4 T cells (CD3 + CD4+), CD8 T cells (CD3 + CD8+), double negative T (DNT) cells (CD3 + CD4-CD8-), B cells (CD3-CD19+), NK cells (CD3-CD56+), and NKT-like cells (CD3 + CD56+) in the peripheral blood of 813 healthy children. We used the method of the international standard document (Clinical Laboratory Standard Institute C28-A3) to establish reference intervals with a single platform.

Effects of Different Factors on Methane Hydrate Formation Using a Visual Wellbore Simulator.

During hydrate exploitation, the formation and decomposition of hydrate in the wellbore are affected by many factors such as salinity, temperature, pressure, gas-liquid ratio, and so on. In the drilling process, inhibitors will be added into the drilling fluid, to prevent the formation of hydrates in the wellbore to form blockages. In order to explore the influence of these factors on the formation and decomposition of hydrates, a visual wellbore simulator was used to study the formation, inhibition, and decomposition of hydrates in the wellbore, which affected these factors.

Application of Improved Robot-assisted Laparoscopic Telesurgery with 5G Technology in Urology.

The demand for telesurgery is rising rapidly, but robust evidence regarding the feasibility of its application in urology is still rare. From March to October 2021, a surgeon-controlled surgical robot in a tertiary hospital in Qingdao was used to remotely conduct robot-assisted laparoscopic radical nephrectomy (RN) in 29 patients located in eight primary hospitals. The median round-trip delay was 26 ms (interquartile range [IQR] 5) and the median distance between the primary hospital and the surgeon was 187 km (IQR 57).

DiT-SLAM: Real-Time Dense Visual-Inertial SLAM with Implicit Depth Representation and Tightly-Coupled Graph Optimization.

Recently, generating dense maps in real-time has become a hot research topic in the mobile robotics community, since dense maps can provide more informative and continuous features compared with sparse maps. Implicit depth representation (e.g.

Reference ranges of T lymphocyte subsets by single-platform among healthy population in southwest China.

Background: Appropriate reference ranges of T lymphocyte subsets are essential for immune status evaluation of patients with immunological diseases. We aim to establish the age- and sex-related reference intervals of T lymphocyte subsets by single-platform for the southwest China population using the indirect method with the data resulting from 53,822 cases of periodic health examination individuals in the Laboratory Information System (LIS) of West China Hospital from 2018 to 2020.

Methods: We used the Box-Cox conversion combined with the Tukey method to normalize the data and eliminate the outliers, and the nonparametric method to estimate the 95% distribution reference intervals.

An autonomous excavator system for material loading tasks.

Excavators are widely used for material handling applications in unstructured environments, including mining and construction. Operating excavators in a real-world environment can be challenging due to extreme conditions-such as rock sliding, ground collapse, or excessive dust-and can result in fatalities and injuries. Here, we present an autonomous excavator system (AES) for material loading tasks.

MLDA-Net: Multi-Level Dual Attention-Based Network for Self-Supervised Monocular Depth Estimation.

The success of supervised learning-based single image depth estimation methods critically depends on the availability of large-scale dense per-pixel depth annotations, which requires both laborious and expensive annotation process. Therefore, the self-supervised methods are much desirable, which attract significant attention recently. However, depth maps predicted by existing self-supervised methods tend to be blurry with many depth details lost.