5-Aminolevulinic acid improves strawberry salt tolerance through a NO-HO signaling circuit regulated by FaWRKY70 and FaWRKY40.

Introduction: 5-Aminolevulinic acid (ALA) is an essential biosynthetic precursor of tetrapyrrole compounds, naturally occurring in all living organisms. It has also been suggested as a new plant growth regulator. Treatment with ALA promotes strawberry Na homeostasis under salt stress.

A green and efficient method for detecting nicosulfuron residues in field maize using hyperspectral imaging and deep learning.

Accurate and rapid detection of nicosulfuron herbicide residues in field-grown maize is essential for implementing chemical remediation and optimizing spraying strategies. However, current detection methods are costly and time-consuming. This study analyzed residue levels in six maize varieties-both resistant and sensitive types-under two herbicide concentrations, categorizing residues into low, medium, and high levels.

MdFC2, a ferrochelatase gene, is a positive regulator of ALA-induced anthocyanin accumulation in apples.

5-Aminolevulinic acid (ALA), a key biosynthetic precursor of tetrapyrrole compounds, significantly induces anthocyanin accumulation in apple (Malus × domestica Borkh.) as well as other fruits. Although the molecular mechanisms of ALA-induced anthocyanin accumulation have been reported, it remains unknown whether the metabolism of ALA is involved in ALA-induced anthocyanin accumulation.

Effects of plasma-activated water combined with ultrasonic treatment of corn starch on structural, thermal, physicochemical, functional, and pasting properties.

In this study, corn starch was used as the raw material, and modified starch was prepared using a method combining plasma-activated water and ultrasound treatment (PUL). This method was compared with treatments using plasma-activated water (PAW) and ultrasound (UL) alone. The structure, thermal, physicochemical, pasting, and functional properties of the native and treated starches were evaluated.

Incorporation of ascorbic acid in chitosan-based coating combined with plasma-activated water: A technology for quality preservation of red grapes after simulated transportation.

Red grapes possess multiple bioactivities but are highly susceptible to spoilage due to the lack of efficient preservation techniques. Plasma-activated water (PAW) treatment and the incorporation of antioxidants in bio-based coatings are promising methods for preserving produce. In this study, we tested a novel combination by incorporating ascorbic acid (AA) into a chitosan-based edible coating (CH) and combining it with plasma-activated water (PAW) treatment (CA-PAW) before simulating transport vibrations to extend the shelf-life of red grapes.

Transcription factor MdNAC33 is involved in ALA-induced anthocyanin accumulation in apples.

5-Aminolevulinic acid (ALA), as a new natural plant growth regulator, has a significant function in promoting anthocyanin accumulation in many species of fruits. However, the mechanisms underlying remain obscure. In a transcriptome study of our group, it was found that many transcription factors (TFs) including NACs responsive to ALA treatment during anthocyanin accumulation.

Dead Laying Hens Detection Using TIR-NIR-Depth Images and Deep Learning on a Commercial Farm.

In large-scale laying hen farming, timely detection of dead chickens helps prevent cross-infection, disease transmission, and economic loss. Dead chicken detection is still performed manually and is one of the major labor costs on commercial farms. This study proposed a new method for dead chicken detection using multi-source images and deep learning and evaluated the detection performance with different source images.

ALA induces stomatal opening through regulation among PTPA, PP2AC, and SnRK2.6.

5-Aminolevulinic acid (ALA), as a new natural plant growth regulator, has been proved to regulate protein phosphatase 2A (PP2A) activity to promote stomatal opening in apple () leaves. However, the molecular mechanisms underlying remain unclear. Here, we cloned and transformed , , and of apple into tobaccos () and found that over-expression (OE)- or OE- promoted stomatal aperture while OE- induced stomatal closure under normal or drought condition.

ALA reverses ABA-induced stomatal closure by modulating PP2AC and SnRK2.6 activity in apple leaves.

5-Aminolevulinic acid (ALA), known as a new natural plant growth regulator, can reverse abscisic acid (ABA)-induced stomatal closure. The protein phosphatase 2A (PP2A) played an important role in regulation of stomatal movement by ALA and ABA; however, the underlying molecular mechanisms remain unclear. Here, we report that ALA promotes MdPP2A activity and gene expression in the leaf epidermis of apple ( Borkh.

Elimination of Leaf Angle Impacts on Plant Reflectance Spectra Using Fusion of Hyperspectral Images and 3D Point Clouds.

During recent years, hyperspectral imaging technologies have been widely applied in agriculture to evaluate complex plant physiological traits such as leaf moisture content, nutrient level, and disease stress. A critical component of this technique is white referencing used to remove the effect of non-uniform lighting intensity in different wavelengths on raw hyperspectral images. However, a flat white tile cannot accurately reflect the lighting intensity variance on plant leaves, since the leaf geometry (e.

Rabbit manure compost as a peat substitute for compound growing media: Proportioning optimization according to physiochemical characteristics and seedling effects.

A large amount of rabbit manure is produced with the development of the rabbit industry, which will cause environmental pollution without proper treatment. Rabbit manure compost may be suitable for seedling cultivation, considering its low moisture, low heavy metal, high lignocellulose, and good fertilizer effect. In this study, a pre-proportioning test of growing media was conducted to optimize the ratio of perlite and vermiculite with peat/rabbit manure compost according to their physicochemical properties.

MdMYB110a, directly and indirectly, activates the structural genes for the ALA-induced accumulation of anthocyanin in apple.

5-Aminolevulinic acid (ALA), an essential biosynthetic precursor of tetrapyrrole compounds, promotes the anthocyanin accumulation in many plant species. However, the underlying mechanism of ALA-induced accumulation is not yet fully understood. In this study, we identified an important regulator of the anthocyanin accumulation, MdMYB110a, which plays an important role in the ALA-induced anthocyanin accumulation.

ALA Upregulates MdPTPA Expression to Increase the PP2A Activity and Promote Stomatal Opening in Apple Leaves.

5-Aminolevulinic acid (ALA) is a new natural plant growth regulator that inhibits abscisic acid (ABA)-induced stomatal closure. Studies have shown that protein phosphatase 2 A (PP2A) is involved in ALA-ABA antagonistically regulating stomatal movement; however, the molecular mechanisms underlying remain unclear. Here, we report that ALA promoted MdPP2A activity and the MdPP2AC expression in the epidermis of apple (Malus × domestica Borkh.

The Transcription Factor MdERF78 Is Involved in ALA-Induced Anthocyanin Accumulation in Apples.

As a friendly plant growth regulator to the environment, 5-aminolevulinic acid (ALA) has been widely used in plant production, such as fruit coloration, stress resistance, and so on. Previous studies have identified some genes that have a function in the anthocyanin accumulation induced by ALA. However, the regulatory mechanism has not been well revealed.

MdSCL8 as a Negative Regulator Participates in ALA-Induced to Promote Flavonol Accumulation in Apples.

Apples () are rich in flavonols, and 5-aminolevulinic acid (ALA) plays an important role in the regulation of plant flavonoid metabolism. To date, the underlying mechanism of ALA promoting flavonol accumulation is unclear. Flavonol synthase (FLS) is a key enzyme in flavonol biosynthesis.

PLS-DA and Vis-NIR spectroscopy based discrimination of abdominal tissues of female rabbits.

Using Vis-NIR spectroscopy to distinguish gestational sac from other abdominal tissues is the key to diagnosing female rabbits' pregnancy by optical means. This study aims to demonstrate the gestational sac and other abdominal tissues (hair, skin, breast, muscle, cecum, small intestine) of rabbits can be identified using Vis-NIR spectroscopy in vitro. These tissues' raw NIR spectra were recorded in the Vis-NIR range (490-940 nm) with interactive mode.

Early pregnancy diagnosis of rabbits: A non-invasive approach using Vis-NIR spatially resolved spectroscopy.

Pregnancy diagnosis is essential for rabbit's reproductive management. The early identification of non-pregnant rabbits allows for earlier re-insemination, increases the service rate, and reduces the laboring interval in commercial operations. The objective of this study was to establish the feasibility of using a Vis-NIR spatially resolved spectroscopy for diagnosing pregnancy in female rabbits.

Transcriptomic Profiling of Apple Calli With a Focus on the Key Genes for ALA-Induced Anthocyanin Accumulation.

The red color is an attractive trait of fruit and determines its market acceptance. 5-Aminolevulinic acid (ALA), an eco-friendly plant growth regulator, has played a universal role in plant secondary metabolism regulation, particularly in flavonoid biosynthesis. It has been widely reported that ALA can up-regulate expression levels of several structural genes related to flavonoid metabolism and anthocyanin accumulation.

Photosynthetic Responses of Canola to Exogenous Application or Endogenous Overproduction of 5-Aminolevulinic Acid (ALA) under Various Nitrogen Levels.

Limited data are available on the effects of 5-aminolevulinic acid (ALA) on plant photosynthesis in relation to the nitrogen (N) level. In this study, we investigate photosynthetic responses to ALA in canola plants ( L.).

Stress Distribution Analysis on Hyperspectral Corn Leaf Images for Improved Phenotyping Quality.

High-throughput imaging technologies have been developing rapidly for agricultural plant phenotyping purposes. With most of the current crop plant image processing algorithms, the plant canopy pixels are segmented from the images, and the averaged spectrum across the whole canopy is calculated in order to predict the plant's physiological features. However, the nutrients and stress levels vary significantly across the canopy.

Genome-wide identification and comparative analysis of GST gene family in apple () and their expressions under ALA treatment.

Anthocyanins, a subclass of flavonoids, are synthesized at the cytoplasmic surface of the endoplasmic reticulum (ER), which then accumulate in vacuoles. Plant glutathione S-transferase () genes are involved in anthocyanin transportation. Here, a total of 52, 42, 50, and 29 genes were identified from apple, pear, peach, and strawberry, respectively, through a comprehensive genome-wide survey.

Precise Estimation of NDVI with a Simple NIR Sensitive RGB Camera and Machine Learning Methods for Corn Plants.

The normalized difference vegetation index (NDVI) is widely used in remote sensing to monitor plant growth and chlorophyll levels. Usually, a multispectral camera (MSC) or hyperspectral camera (HSC) is required to obtain the near-infrared (NIR) and red bands for calculating NDVI. However, these cameras are expensive, heavy, difficult to geo-reference, and require professional training in imaging and data processing.

LeafScope: A Portable High-Resolution Multispectral Imager for In Vivo Imaging Soybean Leaf.

Portable devices for measuring plant physiological features with their isolated measuring chamber are playing an increasingly important role in plant phenotyping. However, currently available commercial devices of this type, such as soil plant analysis development (SPAD) meter and spectrometer, are dot meters that only measure a small region of the leaf, which does not perfectly represent the highly varied leaf surface. This study developed a portable and high-resolution multispectral imager (named LeafScope) to in-vivo image a whole leaf of dicotyledon plants while blocking the ambient light.

Transcriptomic Analysis of Peels with a Focus on the Key Genes for Anthocyanin Biosynthesis.

Fig ( L.), a deciduous fruit tree of the Moraceae, provides ingredients for human health such as anthocyanins. However, little information is available on its molecular structure.

Exogenous 5-aminolevulinic acid improves strawberry tolerance to osmotic stress and its possible mechanisms.

Cultivated strawberry, one of the major fruit crops worldwide, is an evergreen plant with shallow root system, and thus sensitive to environmental changes, including drought stress. To investigate the effect of 5-aminolevulinic acid (ALA), a new environment-friendly plant growth regulator, on strawberry drought tolerance and its possible mechanisms, we treated strawberry (Fragaria × annanasa Duch. cv.