Experimental study on the treatment of deep miscellaneous fill foundation in Xinjiang by vibrating rod compaction method.

Currently, the treatment of miscellaneous fill foundations, composed of a mixture of domestic garbage, construction solid waste, and natural soil, presents a significant challenge in urban peripheral engineering construction. This paper discusses the application of vibrating rod compaction technology for foundation treatment in Xinjiang. It evaluates the effectiveness of cross-section vibrating rod compaction equipment in reinforcing fine-grained miscellaneous fill foundations.

Prediction model of land surface settlement deformation based on improved LSTM method: CEEMDAN-ICA-AM-LSTM (CIAL) prediction model.

The uneven settlement of the surrounding ground surface caused by subway construction is not only complicated but also liable to cause casualties and property damage, so a timely understanding of the ground settlement deformation in the subway excavation and its prediction in real time is of practical significance. Due to the complex nonlinear relationship between subway settlement deformation and numerous influencing factors, as well as the existence of a time lag effect and the influence of various factors in the process, the prediction performance and accuracy of traditional prediction methods can no longer meet industry demands. Therefore, this paper proposes a surface settlement deformation prediction model by combining noise reduction and attention mechanism (AM) with the long short-term memory (LSTM).

Study on mechanical properties of interbedded rock masses with microcrack based on thermal-mechanical coupling.

The mechanical properties of deep rock masses are significantly influenced by temperature and other factors. The effect of temperature on the strength of deep rock masses will pose a serious challenge to deep resource exploitation and engineering construction. In this paper, the thermal-mechanical coupling calculation model is established by particle flow code (PFC2D) to study the uniaxial compression response of rock masses with microcracks after temperature load.

Research on the damage characteristics of rock masses based on double guide-hole blasting under high in-situ stress.

In complex high in-situ stress conditions, how to achieve the ideal rock blasting effect through effective methods is often a difficult point of blasting operations. This paper analyzes the influence of guide holes on blasting effect by adding guide holes to the rock pre-treatment method. Based on the particle expansion method to carry out double-hole blasting experiments, the influence of the blasthole spacing and ground stress on the blasting effect is investigated from the levels of macroscopic cracking effect, microscopic particle contact and so on.

Influence of external insert angle on structural deformation of long pipe shed with shallow hole.

The long pipe shed advanced support has the characteristics of large construction difficulty, wide support range, and easy deviation. The difference in construction dip angle will produce a different plastic zone of surrounding rock and supporting effects, and the rationality of advanced support design plays an important role in the safety of tunnel opening excavation. Based on the Tianshan Tunnel project, this paper aims at the problems of the loose pebble layer structure, poor cohesion, strong permeability, shallow excavation is not easy to form a confined arch, and easy to causes surrounding rock deformation and failure.

DEM modeling direct shearing behavior of sand considering anti-rotation of particle.

Considering anti-rotation of sand particles, two-dimensional Discrete Element Method (DEM) has been employed to reproduce direct shear behaviors of sand with different particle distribution sizes, so as to explore effects of anti-rotation of particle on responses of stress-displacement and dilatancy, the evolution law of shear stress, coordination number and vertical displacement of sand samples, and analyze the contact force chain, contact fabric and porosity of the samples after shearing.The results show that the anti-rotation ability of sand is enhanced, the torque of overcoming the relative rotation between particles is increased, and the peak shear stress, dilatancy and porosity in the middle of the sample are increased; with the increase of the anti-rotation coefficient, the coordination number decreases more obviously. The proportion of the contact number in the direction of 100°-160° to the total contact number decreases with the increase of the anti-rotation coefficient.

Cracks propagation characteristics of double-hole delay blasting in soft-hard composite rock mass.

By researching the distance between blasthole and interface of soft-hard rock strata, as well as the time of delay detonation, blasting effect of the rock mass will be more controllable. Firstly, validity of numerical method was authenticated from three angles: blasting coupled stress field, ratio of crushing zone radius to blasthole radius, and crack network state. Under the condition of soft-hard rock strata, numerical model of double-hole blasting was established by using PFC.

Study of snow cover/depth evolution characteristics in Tianshan region of China based on geographical partition.

Based on the digital elevation data, snow depth and snow cover remote sensing data, this paper divides six snow evolution areas and geographical partitions, extracts the geographical partitions of each evolution area and obtains the geographical characteristics of the evolution area for analysis. The results show that: (1) From 2003 to 2017, the average snow area decreased at a rate of - 0.004, and the average snow depth increased at a rate of 0.

Numerical simulation of size effect of defective rock under compression condition.

The existence of various types of damage, small cracks, some large voids and the size of the sample in the rock will make the experimental results show great discreteness. In this paper, based on the results of laboratory experiments, a numerical model of large flawed rock samples is established by using particle flow software PFC2D, and the mechanical response of rocks with different length-diameter ratios and different flaw positions in uniaxial compression experiments is discussed. The results show that the specimen size has a significant effect on the crack characteristics, mechanical characteristics and energy characteristics of rock mass.

Study on blasting characteristics of rock mass with weak interlayer based on energy field.

In order to explore the influence of weak interlayer on blasting characteristics in natural rock mass, by using the particle flow code (PFC), a single hole blasting numerical model of hard rock with soft interlayer is established. The blasting experiments at different positions and thicknesses of weak interlayer are carried out. Then an in-depth analysis from the perspectives of crack effect, stress field and energy field is made.

CRISPR-Edited Stem Cells in a Patient with HIV and Acute Lymphocytic Leukemia.

The safety of CRISPR (clustered regularly interspaced short palindromic repeats)-based genome editing in the context of human gene therapy is largely unknown. is a reasonable but not absolutely protective target for a cure of human immunodeficiency virus type 1 (HIV-1) infection, because -null blood cells are largely resistant to HIV-1 entry. We transplanted CRISPR-edited -ablated hematopoietic stem and progenitor cells (HSPCs) into a patient with HIV-1 infection and acute lymphoblastic leukemia.

Correction to: Efficient derivation of extended pluripotent stem cells from NOD-scid Il2rg mice.

In the original publication Fig. 1D and supplementary material is incorrect. The correct figure and supplementary material is provided in this correction.

Rapid generation of gene-targeted EPS-derived mouse models through tetraploid complementation.

One major strategy to generate genetically modified mouse models is gene targeting in mouse embryonic stem (ES) cells, which is used to produce gene-targeted mice for wide applications in biomedicine. However, a major bottleneck in this approach is that the robustness of germline transmission of gene-targeted ES cells can be significantly reduced by their genetic and epigenetic instability after long-term culturing, which impairs the efficiency and robustness of mouse model generation. Recently, we have established a new type of pluripotent cells termed extended pluripotent stem (EPS) cells, which have superior developmental potency and robust germline competence compared to conventional mouse ES cells.

Efficient derivation of extended pluripotent stem cells from NOD-scid Il2rg mice.

Recently we have established a new culture condition enabling the derivation of extended pluripotent stem (EPS) cells, which, compared to conventional pluripotent stem cells, possess superior developmental potential and germline competence. However, it remains unclear whether this condition permits derivation of EPS cells from mouse strains that are refractory or non-permissive to pluripotent cell establishment. Here, we show that EPS cells can be robustly generated from non-permissive NOD-scid Il2rg mice through de novo derivation from blastocysts.

CRISPR/Cas9-Mediated CCR5 Ablation in Human Hematopoietic Stem/Progenitor Cells Confers HIV-1 Resistance In Vivo.

Transplantation of hematopoietic stem cells (HSCs) with a naturally occurring CCR5 mutation confers a loss of detectable HIV-1 in the patient, making ablation of the CCR5 gene in HSCs an ideal therapy for an HIV-1 cure. Although CCR5 disruption has been attempted in CD4 T cells and hematopoietic stem/progenitor cells (HSPCs), efficient gene editing with high specificity and long-term therapeutic potential remains a major challenge for clinical translation. Here, we established a CRISPR/Cas9 gene editing system in human CD34 HSPCs and achieved efficient CCR5 ablation evaluated in long-term reconstituted NOD/Prkdc/IL-2Rγ mice.

Cell cycle arrest induced by inhibitors of epigenetic modifications in maize (Zea mays) seedling leaves: characterization of the process and possible mechanisms involved.

Epigenetic modifications play crucial roles in the regulation of chromatin architecture and are involved in cell cycle progression, including mitosis and meiosis. To explore the relationship between epigenetic modifications and the cell cycle, we treated maize (Zea mays) seedlings with six different epigenetic modification-related inhibitors and identified the postsynthetic phase (G2 ) arrest via flow cytometry analysis. Total H4K5ac levels were significantly increased and the distribution of H3S10ph signalling was obviously changed in mitosis under various treatments.

Multilayered molecular profiling supported the monoclonal origin of metastatic renal cell carcinoma.

Primary renal cell carcinomas (pRCCs) have a high degree of intratumoral heterogeneity and are composed of multiple distinct subclones. However, it remains largely unknown that whether metastatic renal cell carcinomas (mRCCs) also have startling intratumoral heterogeneity or whether development of mRCCs is due to early dissemination or late diagnosis. To decipher the evolution of mRCC, we analyzed the multilayered molecular profiles of pRCC, local invasion of the vena cava (IVC), and distant metastasis to the brain (MB) from the same patient using whole-genome sequencing, whole-exome sequencing, DNA methylome profiling, and transcriptome sequencing.

Whole-genome and whole-exome sequencing of bladder cancer identifies frequent alterations in genes involved in sister chromatid cohesion and segregation.

Bladder cancer is one of the most common cancers worldwide, with transitional cell carcinoma (TCC) being the predominant form. Here we report a genomic analysis of TCC by both whole-genome and whole-exome sequencing of 99 individuals with TCC. Beyond confirming recurrent mutations in genes previously identified as being mutated in TCC, we identified additional altered genes and pathways that were implicated in TCC.